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TLE2 antibody

Principal name

TLE2 antibody

Alternative names for TLE2 antibody

Transducin-like enhancer protein 2, TLE2, GRG2

SwissProt ID

Q04725 (Human), Q9WVB2 (Mouse)

Gene ID

7089 (TLE2), 21886 (Tle2)

Ncbi ID

NP_001138233.1, NP_001138233, NP_003251, 9606, NM_003260, NM_001144762, NP_062699.1, NM_019725

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TLE2 antibody

The Notch signaling pathway controls cellular interactions important for the specification of a variety of fates in both invertebrates and vertebrates. Key players in the Notch pathway are the TLE genes (for transducin-like enhancer of split, also designated ESG for enhancer of split groucho), which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. TLEs associate with chromatin in live cells and specifically with Histone H3, but not with other core histones. Expression of the TLE genes, TLE1, TLE2, TLE3 and TLE4, correlate with immature epithelial cells that are progressing toward a terminally differentiated state, suggesting a role during epithelial differentiation. TLE1, TLE2 and TLE3 have elevated expression in cervial squamous metaplasias and carcinomas, while TLE4 is most highly expressed in the brain, particularly in the caudate nucleus. TLE1 and TLE4 contain SP and WD40 domains, through which TLE1 binds AML1 to inhibit AML1-induced transactivation of the CSF1 receptor.

General readings

Higa,L.A., (2006) Nat. Cell Biol. 8 (11), 1277-1283

Western blot (WB) analysis of TLE2 antibody (Cat.-No.: AP06713PU-N) in extracts from hela cells.

AP06713PU-N

TLE2 Antibody (C-term) (AP54267PU-N) western blot analysis in mouse lung tissue lysates (35 µg/lane). This demonstrates the TLE2 antibody detected the TLE2 protein (arrow).

AP54267PU-N

Transfected 293T; WB Suggested Anti-TLE2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T; TLE2 antibody - N-terminal region (ARP32347_P050) in Transfected 293T cells using Western Blot

ARP32347_P050

Western blot analysis of extracts from LOVO cells, using TLE2 Antibody. The lane on the right is treated with the synthesized peptide.

Western Blot analysis of TLE2 expression in transfected 293T cell line (H00007089-T01) by TLE2 MaxPab polyclonal antibody.Lane 1: TLE2 transfected lysate(81.73 KDa).Lane 2: Non-transfected lysate.

H00007089-B01

Immunohistochemical analysis of TLE2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of TLE2 expression in HeLa (A); Jurkat (B) whole cell lysates.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TLE2 mouse monoclonal antibody. (TA504240)

TA504240

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TLE2 mouse monoclonal antibody. (TA504240)

TA504240

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TLE2 mouse monoclonal antibody. (TA504240)

TA504240

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TLE2 mouse monoclonal antibody. (TA504240)

TA504240

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TLE2 mouse monoclonal antibody. (TA504240)

TA504240

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TLE2 mouse monoclonal antibody. (TA504240)

TA504240

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TLE2 mouse monoclonal antibody. (TA504240)

TA504240

Anti-TLE2 mouse monoclonal antibody (TA504240) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TLE2(RC202474).

TA504240

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLE2 (RC202474, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TLE2.

TA504240

Flow cytometric Analysis of Hela cells, using anti-TLE2 antibody(TA504240),(Red), compared to a nonspecific negative control antibody,(Blue).

TA504240

Flow cytometric Analysis of Jurkat cells, using anti-TLE2 antibody(TA504240),(Red), compared to a nonspecific negative control antibody,(Blue).

TA504240

HEK293T cells transfected with either RC202474 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TLE2 antibody(TA504240), and then analyzed by flow cytometry.

TA504240

TP302474

Western blot analysis of extracts from LOVO cells, using TLE2 antibody.The lane on the right is treated with the synthesized peptide.

TA312089

WB Suggested Anti-TLE2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Transfected 293T

TA330263

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA504240	TLE2 antibody
	TLE2 mouse monoclonal antibody, clone OTI5A6 (formerly 5A6)
	Mouse	IgG1	OTI5A6	Purified	Hu	F, ICC/IF, P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+11 additional images	Highly validated antibody!
TA312089	TLE2 antibody
	Rabbit polyclonal anti-TLE2 antibody
	Rabbit	IgG		Purified	Hu	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
AP06713PU-N	TLE2 antibody
	Rabbit			Aff - Purified	Hu	E, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
AP54267PU-N	TLE2 (C-term) antibody
	Rabbit	Ig		Aff - Purified	Hu, Ms	WB	0.4 ml / €415.00
							OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP302474	TLE2 (transcript variant 1)
	Recombinant protein of human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY401124	TLE2 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY428507	TLE2 overexpression lysate
		0.1 mg / €315.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR215597L2V	Lenti ORF particles, Tle2 (GFP-tagged) - Mouse transducin-like enhancer of split 2, homolog of Drosophila E(spl) (Tle2), 200 uL, >10^7 TU/mL
		200 µl / €1,560.00
		OriGene Technologies, Inc.

MR215597L1V	Lenti ORF particles, Tle2 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 2, homolog of Drosophila E(spl) (Tle2), 200 uL, >10^7 TU/mL
		200 µl / €1,400.00
		OriGene Technologies, Inc.

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