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TMEM38A antibody

Principal name

TMEM38A antibody

Alternative names for TMEM38A antibody

Transmembrane protein 38A, TRICA, TRIC-A, Trimeric intracellular cation channel type A

SwissProt ID

A4FV75 (Bovin), A5A6S6 (Rabit), A6ZIQ8 (Rat), Q3TMP8 (Mouse), Q5ZK43 (Chick), Q9H6F2 (Human)

Gene ID

79041 (TMEM38A), 306327 (Tmem38a), 74166

Ncbi ID

9606, NP_076979, NP_076979.1, NM_024074, AAH01195, NP_001093645.1, NM_144534, NM_001100175

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TMEM38A antibody

Intracellular Ca2+ levels are important in proper cellular functions and have prominent roles in various cell sigling pathways and are crucial for muscle contractions. Indeed, an important step leading to muscle contraction is the massive release of Ca2+ ions from the endoplasmic /sarcoplasmic reticulum (ER/SR) to the cytosol. A battery of results suggest that specific K+ channels are important to counteract the Ca2+ outflow in order to neutralize the negative potential created by the movement of Ca2+ ions. It is believed that TRIC channels are responsible for neutralizing this negative potential. Trimeric intracellular cation-specific (TRIC) channels are critical for proper magement of intracellular stores. TRIC-A and TRIC-B both belong to this family and are both permeable to monovalent ions with a preference for K+ . Both channels are localized to the ER/SR membrane. Each TRIC subunit contains three transmembrane domains, a cytoplasmic C-terminus and a lumil N-terminus. Functiol entities are formed by homotrimerizarion. The activity of TRIC-A is regulated by voltage whereas that of TRIC-B can be regulated by different mechanisms. Knock out studies of these channels have shown that TRIC-A knock mice are viable while those of TRIC-B die at the neotal stage. TRIC-A is mostly expressed in excitable tissues like the brain and muscle while TRIC-B is ubiquitously expressed.

Human Muscle; WB Suggested Anti-TMEM38A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; TMEM38A antibody - N-terminal region (ARP49799_P050) in Human Muscle cells using Western Blot

ARP49799_P050

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23924PU-N

Western blot analysis of TMEM38A in rat skeletal muscle tissue lysate with TMEM38A antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

AP23924PU-N

TP300985

Western blot analysis of TMEM38A in rat skeletal muscle tissue lysate with TMEM38A antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

TA306888

Immunofluorescence of TMEM38A in mouse skeletal muscle tissue with TMEM38A antibody at 20 ug/mL.

TA306888

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and SH-SY5Y (lanes 3 and 6) lysates: 1-3. Anti-TRIC-B antibody, (1:200).4-6. Anti-TRIC-B antibody, preincubated with the control peptide antigen.

TA329061

Expression of TRIC-B in rat testis. Immunohistochemical staining of rat testis paraffin-embedded sections using Anti-TRIC-B antibody, followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody. A. TRIC-B labeling appears in the columnar principal epithelium of the epididymis and in the endothelium of the surrounding blood vessels. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.

TA329061

Expression of TRIC-B in mouse muscle myoblast (C2C12) cell line. Immunocytochemical staining of mouse paraformaldehyde-fixed and permeabilized muscle myoblast (C2C12) cell line. A. Cells were stained with Anti-TRIC-B antibody, (1:200) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.

TA329061

Western blot analysis of rat brain lysate (lanes 1 and 4) and mouse brain membrane (lanes 2 and 5) and skeletal muscle (lanes 3 and 6): 1-3. Anti-TRIC-A antibody, (1:200). 4-6. Anti-TRIC-A antibody, preincubated with the control peptide antigen.

TA329062

Expression of TRIC-A in rat skeletal muscle. Immunohistochemical staining of paraffin-embedded rat skeletal muscle sections using Anti-TRIC-A antibody, followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. TRIC-A labeling (red) appears in the edges of the muscle fibers, where the endomysium is present. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.

TA329062

Expression of TRIC-A in mouse muscle myoblast (C2C12) cell line. Immunocytochemical staining of mouse paraformaldehyde-fixed and permeabilized muscle myoblast (C2C12) cell line. A. Cells were stained with Anti-TRIC-A antibody, (1:200) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.

TA329062

WB Suggested Anti-TMEM38A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Muscle

TA338647

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA306888	TMEM38A antibody
	Rabbit Polyclonal TMEM38A Antibody
	Rabbit	IgG	Aff - Purified	Hu, Ms, Rt	E, ICC/IF, WB	0.1 mg / €415.00
						OriGene Technologies, Inc.
+1 additional image
TA329062	TMEM38A antibody
	Rabbit polyclonal Anti-TRIC-A
	Rabbit		Purified	Ms, Rt	WB	0.2 ml / €585.00
						OriGene Technologies, Inc.
+2 additional images
AP23924PU-N	TMEM38A (C-term) antibody
	Rabbit		Purified	Hu, Ms, Rt	E, P, WB	50 µg / €425.00
						OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP300985	TMEM38A
	Recombinant protein of human transmembrane protein 38A (TMEM38A)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY411389	TMEM38A overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR204130L2V	Lenti ORF particles, Tmem38a (GFP-tagged) - Mouse transmembrane protein 38A (Tmem38a), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

MR204130L1V	Lenti ORF particles, Tmem38a (Myc-DDK-tagged) - Mouse transmembrane protein 38A (Tmem38a), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR211863L2V	Lenti ORF particles, Tmem38a (GFP-tagged ORF) - Rat transmembrane protein 38a (Tmem38a), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR211863L1V	Lenti ORF particles, Tmem38a (Myc-DDK-tagged ORF) - Rat transmembrane protein 38a (Tmem38a), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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