Home » Target » TMEM38B antibody

TMEM38B antibody

Principal name

TMEM38B antibody

Alternative names for TMEM38B antibody

Transmembrane protein 38B, TRICB, TRIC-B, C9orf87, Trimeric intracellular cation channel type B

SwissProt ID

Q0VC58 (Bovin), Q68FV1 (Rat), Q9DAV9 (Mouse), Q9NVV0 (Human)

Gene ID

55151 (TMEM38B), 362521 (Tmem38b), 52076

Ncbi ID

9606, NP_060582, NP_060582.1, NM_018112, NP_001014213.1, NM_028053, NM_001014191

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of TMEM38B antibody

Intracellular Ca2+ levels are important in proper cellular functions and have prominent roles in various cell sigling pathways and are crucial for muscle contractions. Indeed, an important step leading to muscle contraction is the massive release of Ca2+ ions from the endoplasmic /sarcoplasmic reticulum (ER/SR) to the cytosol. A battery of results suggest that specific K+ channels are important to counteract the Ca2+ outflow in order to neutralize the negative potential created by the movement of Ca2+ ions. It is believed that TRIC channels are responsible for neutralizing this negative potential. Trimeric intracellular cation-specific (TRIC) channels are critical for proper magement of intracellular stores. TRIC-A and TRIC-B both belong to this family and are both permeable to monovalent ions with a preference for K+ . Both channels are localized to the ER/SR membrane. Each TRIC subunit contains three transmembrane domains, a cytoplasmic C-terminus and a lumil N-terminus. Functiol entities are formed by homotrimerizarion. The activity of TRIC-A is regulated by voltage whereas that of TRIC-B can be regulated by different mechanisms. Knock out studies of these channels have shown that TRIC-A knock mice are viable while those of TRIC-B die at the neotal stage. TRIC-A is mostly expressed in excitable tissues like the brain and muscle while TRIC-B is ubiquitously expressed.

Human HeLa; WB Suggested Anti-TMEM38B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; TMEM38B antibody - C-terminal region (ARP47395_P050) in Human HeLa cells using Western Blot

ARP47395_P050

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23925PU-N

Western blot analysis of TMEM38B in rat thymus tissue lysate with TMEM38B antibody at (A) 1 and (B) 2 µg/ml.

AP23925PU-N

TP300137

Western blot analysis of TMEM38B in rat thymus tissue lysate with TMEM38B antibody at (A) 1 and (B) 2 ug/mL.

TA306889

Immunohistochemistry of TMEM38B in mouse thymus tissue with TMEM38B antibody at 5 ug/mL.

TA306889

Immunofluorescence of TMEM38B in mouse thymus tissue with TMEM38B antibody at 20 ug/mL.

TA306889

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and SH-SY5Y (lanes 3 and 6) lysates: 1-3. Anti-TRIC-B antibody, (1:200).4-6. Anti-TRIC-B antibody, preincubated with the control peptide antigen.

TA329060

Expression of TRIC-B in rat testis. Immunohistochemical staining of rat testis paraffin-embedded sections using Anti-TRIC-B antibody, followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody. A. TRIC-B labeling appears in the columnar principal epithelium of the epididymis and in the endothelium of the surrounding blood vessels. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.

TA329060

Expression of TRIC-B in mouse muscle myoblast (C2C12) cell line. Immunocytochemical staining of mouse paraformaldehyde-fixed and permeabilized muscle myoblast (C2C12) cell line. A. Cells were stained with Anti-TRIC-B antibody, (1:200) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.

TA329060

WB Suggested Anti-TMEM38B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysate

TA342357

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA306889	TMEM38B antibody
	Rabbit Polyclonal TMEM38B Antibody
	Rabbit	IgG	Aff - Purified	Hu, Ms, Rt	E, P, WB	0.1 mg / €415.00
						OriGene Technologies, Inc.
+2 additional images
TA329060	TMEM38B antibody
	Rabbit polyclonal Anti-TRIC-B
	Rabbit		Purified	Hu, Ms, Rt	WB	50 µl / €465.00
						OriGene Technologies, Inc.
+2 additional images
AP23925PU-N	TMEM38B (C-term) antibody
	Rabbit		Purified	Hu, Ms, Rt	E, P, WB	50 µg / €425.00
						OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP300137	TMEM38B
	Recombinant protein of human transmembrane protein 38B (TMEM38B)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY413299	TMEM38B overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR204011L2V	Lenti ORF particles, Tmem38b (GFP-tagged) - Mouse transmembrane protein 38B (Tmem38b), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

MR204011L1V	Lenti ORF particles, Tmem38b (Myc-DDK-tagged) - Mouse transmembrane protein 38B (Tmem38b), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR211035L2V	Lenti ORF particles, Tmem38b (GFP-tagged ORF) - Rat transmembrane protein 38B (Tmem38b), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR211035L1V	Lenti ORF particles, Tmem38b (Myc-DDK-tagged ORF) - Rat transmembrane protein 38B (Tmem38b), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

Antibodies (see all»)

Kits (see all»)

Antigens

Lysates (see all»)

Tissue Slides (see all»)

Other life-science products