TNFAIP8 antibody
Principal name
TNFAIP8 antibody
Alternative names for TNFAIP8 antibody
SCC-S2, NDED, MDC-3.13, Tumor necrosis factor alpha-induced protein 8, Head and neck tumor and metastasis-related protein, TNF-induced protein GG2-1, NF-kappa-B-inducible DED-containing protein
Ncbi ID
9606, NP_001071122.1, NP_001071122, NP_055165, NM_014350, NM_001077654, NM_134131, NM_001177759, NM_001177760, NP_001171231.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of TNFAIP8 antibody
- Kit Component:
- - KN317969G1, Tnfaip8 gRNA vector 1 in pCas-Guide vector
- KN317969G2, Tnfaip8 gRNA vector 2 in pCas-Guide vector
- KN317969D, donor vector containing left and right homologous arms and RFP-BSD functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kumar D, Gokhale P, et al. (2004). Oncogene. 23(2):612-6.
Western Blot analysis of TNFAIP8 expression in transfected 293T cell line (H00025816-T01) by TNFAIP8 MaxPab polyclonal antibody.Lane 1: TNFAIP8 transfected lysate(20.9 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of TNFAIP8 expression in transfected 293T cell line (H00025816-T01) by TNFAIP8 MaxPab polyclonal antibody.Lane 1: TNFAIP8 transfected lysate(20.9 KDa).Lane 2: Non-transfected lysate.
TNFAIP8 Antibody (N-term) (AP54304PU-N) western blot analysis in K562 cell line lysates (35 µg/lane). This demonstrates the TNFAIP8 antibody detected the TNFAIP8 protein (arrow).
TNFAIP8 Antibody (N-term) (AP54304PU-N) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of A549 cells, using TFIP8 Antibody Cat.-No AP32253PU-N . The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HuvEc cells using TFIP8 Antibody Cat.-No AP32253PU-N. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of SCC-S2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of SCC-S2 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of SCC-S2 expression in K562 (A); Raw264.7 (B) whole cell lysates.
Western blot analysis of extracts from HUVEC cells, using TFIP8 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using TFIP8 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using TFIP8 antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis on HuvEc cell lysate using TFIP8 Antibody
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (TNFAIP8 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (TNFAIP8 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315288 | TNFAIP8 antibody |
||||||
| Rabbit polyclonal anti-TFIP8 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| TA347583 | TNFAIP8 antibody |
||||||
| Rabbit Polyclonal Anti-TFIP8 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA350527 | TNFAIP8 antibody |
||||||
| Rabbit Polyclonal Anti-TNFAIP8 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AP32253PU-N | TNFAIP8 antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, ICC/IF, P, WB |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
|||||||
| AP54304PU-N | TNFAIP8 (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302729 | TNFAIP8 (transcript variant 1) |
||||
| Recombinant protein of human tumor necrosis factor, alpha-induced protein 8 (TNFAIP8), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP320669 | TNFAIP8 (transcript variant 2) |
||||
| Purified recombinant protein of Homo sapiens tumor necrosis factor, alpha-induced protein 8 (TNFAIP8), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402318 | TNFAIP8 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY421470 | TNFAIP8 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY425876 | TNFAIP8 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201983L2V | Lenti ORF particles, Tnfaip8 (GFP-tagged) - Mouse tumor necrosis factor, alpha-induced protein 8 (Tnfaip8), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201983L1V | Lenti ORF particles, Tnfaip8 (Myc-DDK-tagged) - Mouse tumor necrosis factor, alpha-induced protein 8 (Tnfaip8), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||