TOP3B antibody
Principal name
TOP3B antibody
Alternative names for TOP3B antibody
TOP3B1, DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1
Ncbi ID
9606, NP_003926.1, NP_003926, NP_035754.1, NM_011624, NM_003935
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)
Background of TOP3B antibody
- Kit Component:
- - KN318062G1, Top3b gRNA vector 1 in pCas-Guide vector
- KN318062G2, Top3b gRNA vector 2 in pCas-Guide vector
- KN318062D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western blot analysis of TOP3B over-expressed 293 cell line, cotransfected with TOP3B Validated Chimera RNAi ( Cat # H00008940-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOP3B monoclonal antibody (M01), clone 4F11 (Cat # H00008940-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M01), clone 4F11.Lane 1: TOP3B transfected lysate(96.7 KDa).Lane 2: Non-transfected lysate.
TOP3B monoclonal antibody (M01), clone 4F11 Western Blot analysis of TOP3B expression in K-562 ( Cat # L009V1 ).
Detection limit for recombinant GST tagged TOP3B is approximately 1ng/ml as a capture antibody.
Western Blot analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M03), clone 1H8.Lane 1: TOP3B transfected lysate(96.662 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of monoclonal antibody to TOP3B on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M04), clone 1H4.Lane 1: TOP3B transfected lysate(96.662 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of monoclonal antibody to TOP3B on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M05), clone 4H7.Lane 1: TOP3B transfected lysate(96.662 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M06), clone 2G2.Lane 1: TOP3B transfected lysate(96.662 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of TOP3B expression in transfected 293T cell line (H00008940-T01) by TOP3B MaxPab polyclonal antibody.Lane 1: TOP3B transfected lysate(94.82 KDa).Lane 2: Non-transfected lysate.
Western blot (WB) analysis of Topo IIIβ-1 antibody (Cat.-No.: AP06654PU-N) in extracts from HepG2 cells.
Western Blot analysis of TOP3B expression in transfected 293T cell line (H00008940-T02) by TOP3B MaxPab polyclonal antibody.Lane 1: TOP3B transfected lysate(96.70 KDa).Lane 2: Non-transfected lysate.
TOP3B Antibody (C-term) (AP54334PU-N) western blot analysis in mouse bladder tissue lysates (35 µg/lane). This demonstrates the TOP3B antibody detected the TOP3B protein (arrow).
Immunohistochemical analysis of Topoisomerase 3 beta1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Topoisomerase 3 beta1 expression in HepG2 (A); A431 (B); HeLa (C) whole cell lysates.
Western blot analysis of extracts from HepG2 cells, COLO205 cells and HUVEC cells, using TOP3B antibodyThe lane on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314839 | TOP3B antibody |
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| Rabbit polyclonal anti-TOP3B antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| AP06654PU-N | TOP3B antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| AP54334PU-N | TOP3B (C-term) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210989L1V | Lenti ORF particles, Top3b (Myc-DDK-tagged) - Mouse topoisomerase (DNA) III beta (Top3b), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,530.00
|
|
| OriGene Technologies, Inc. | ||