TP53BP1 antibody
Principal name
TP53BP1 antibody
Alternative names for TP53BP1 antibody
p53-binding protein 1, p53BP1, 53BP1, Tumor suppressor p53-binding protein 1
Ncbi ID
9606, NP_001135451, NP_001135451.1, NM_005657, NP_005648, NP_038763.3, BC035206
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Frozen Sections (C)
Background of TP53BP1 antibody
53BP1 (p53 binding protein 1) plays a key role in response to D damage, checkpoint sigling during mitosis and enhancing TP53-mediated transcriptiol activation. Origilly identified as p53's transcriptiol enhancing partner, 53BP1 now has been established as a substrate for ATM (ataxia telangiectasia mutated) sigling and that it relocalizes to discrete foci overlapping with gamma H2AX (phosphorylated histone H2AX); demarcating D double strand breaks (DSBs) sites following exposure to radiation. 53BP1 functions downstream of gamma H2AX-dependent hierarchy of proteins that collectively establish IRIF (ionizing radiation induced foci) at DSBs; this hierarchy includes Mre11/Rad50/NBS1 (MRN complex), ATM, MDC1, RNF8, RNF168 and HERC2. With the exception of ATM, whose function to generate gamma H2AX may be partially compensated by the activity of D-PK (D-dependent kise), all of these proteins are physically and functiolly required to recruit 53BP1 to the DSB site. Briefly, this process involves DSB recognition by MRN, ATM activation, gamma H2AX-formation, MDC1-recruitment, MRN-retention (leading to further ATM-activation and gamma H2AX spreading) and RNF8/RNF168/HERC2-mediated histone H2A and H2AX mono and poly-ubiquitition.







Lane 1: Hela cell lysate.
Lane 2: sp2/0 cell lysate.
Lane 3: Rat Kidney tissue lysate.
Lane 4: PC12 cell lysate.

























































- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA337093 | TP53BP1 antibody |
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| Mouse Monoclonal 53BP1 Antibody (6B3E10) | |||||||
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Mouse | IgG1 | 6B3E10 | Purified | Hu | C, F, WB |
0.1 ml /
€415.00
|
| OriGene Technologies, Inc. | |||||||
+4 additional images |
Highly validated antibody! |
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| TA807033 | TP53BP1 antibody |
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| TP53BP1 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6) | |||||||
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Mouse | IgG1 | OTI2H6 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
+17 additional images |
Highly validated antibody! |
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| TA807220 | TP53BP1 antibody |
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| TP53BP1 mouse monoclonal antibody, clone OTI5E1 (formerly 5E1) | |||||||
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Mouse | IgG1 | OTI5E1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
+18 additional images |
Highly validated antibody! |
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| TA309917 | TP53BP1 antibody |
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| Rabbit Polyclonal 53BP1 Antibody | |||||||
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Rabbit | Purified | Hu, Ms | C, ICC/IF, WB |
0.1 ml /
€475.00
|
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| OriGene Technologies, Inc. | |||||||
+2 additional images |
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| TA309918 | TP53BP1 antibody |
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| Rabbit Polyclonal 53BP1 Antibody | |||||||
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Rabbit | Purified | Hu, Ms | F, ICC/IF, WB |
0.1 ml /
€475.00
|
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| OriGene Technologies, Inc. | |||||||
+3 additional images |
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| TA312841 | TP53BP1 antibody |
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| Rabbit polyclonal 53BP1 (Ab-29) antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
+1 additional image |
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| TA312842 | TP53BP1 antibody |
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| Rabbit polyclonal anti-53BP1 antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA312844 | TP53BP1 antibody |
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| Rabbit polyclonal 53BP1 (Ser25) antibody(Phospho-specific) | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
+1 additional image |
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| TA332891 | TP53BP1 antibody |
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| Rabbit anti-TP53BP1 Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
+1 additional image |
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| TA348022 | TP53BP1 antibody |
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| Rabbit Polyclonal Anti-53BP1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA807151 | TP53BP1 antibody |
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| TP53BP1 mouse monoclonal antibody, clone OTI12D4 (formerly 12D4) | |||||||
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Mouse | IgG2b | OTI12D4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| AP20288PU-N | TP53BP1 antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
+1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP318016 | TP53BP1 (transcript variant 3) |
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| Recombinant protein of human tumor protein p53 binding protein 1 (TP53BP1), transcript variant 3 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC218016L1V | Lenti ORF particles, TP53BP1 (Myc-DDK tagged) - Human tumor protein p53 binding protein 1 (TP53BP1), transcript variant 3, 200 uL, >10^7 TU/mL |
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200 µl /
€2,420.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211448L2V | Lenti ORF particles, Trp53bp1 (GFP-tagged) - Mouse transformation related protein 53 binding protein 1 (cDNA clone MGC:30704 IMAGE:3979746), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,890.00
|
|
| OriGene Technologies, Inc. | ||
| MR211448L1V | Lenti ORF particles, Trp53bp1 (Myc-DDK-tagged) - Mouse transformation related protein 53 binding protein 1 (cDNA clone MGC:30704 IMAGE:3979746), 200 uL, >10^7 TU/mL |
|
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200 µl /
€1,700.00
|
|
| OriGene Technologies, Inc. | ||















![Western Blot: 53BP1 Antibody (6B3E10) [TA337093] - Western blot analysis using TP53BP1 mAb against HEK293 (1) and TP53BP1 (AA: 574-773)-hIgGFc transfected HEK293 (2) cell lysate.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP2-25028-1-W.jpg)
+4 additional images



![Western Blot: 53BP1 Antibody - Detection of Human [deleted mouse] 53BP1 by Western Blot Sample: Whole cell lysate (20 ug/lane) from U2OS or 293T cells resolved on a 3 to 8% tris-acetate gel. Anti-53BP1 used at 0.5 ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-304-1-W.jpg)








