TPTE antibody
Principal name
TPTE antibody
Alternative names for TPTE antibody
BJ-HCC-5, Putative tyrosine-protein phosphatase TPTE, Transmembrane phosphatase with tensin homology, Cancer/testis antigen 44
SwissProt ID
P56180 (Human)
Gene ID
7179 (TPTE)
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of TPTE antibody
- Kit Component:
- - KN206018G1, TPTE gRNA vector 1 in pCas-Guide vector
- KN206018G2, TPTE gRNA vector 2 in pCas-Guide vector
- KN206018D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Tapparel,C., et al., (2003) Gene 323, 189-199
Human Pancreas; TPTE antibody - C-terminal region (ARP36750_T100) in Human Pancreas cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-TPTE Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; TPTE antibody - C-terminal region (ARP36750_T100) in Human HepG2 cells using Western Blot
TPTE polyclonal antibody ( Cat # PAB7091 ) ( 1 µg/ml ) staining of human testis lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Detection limit for recombinant GST tagged TPTE is approximately 3ng/ml as a capture antibody.
Human Jurkat; WB Suggested Anti-TPTE Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; TPTE antibody - C-terminal region (ARP36747_T100) in Human Jurkat cells using Western Blot
Western Blot analysis of TPTE expression in transfected 293T cell line (H00007179-T01) by TPTE MaxPab polyclonal antibody.Lane 1: TPTE transfected lysate(58.63 KDa).Lane 2: Non-transfected lysate.
FACS analysis of negative control 293 cells (Black) and TPTE expressing 293 cells (Green) using TPTE purified MaxPab mouse polyclonal antibody.
Human PANC1; WB Suggested Anti-TPTE Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: PANC1 cell lysate; TPTE antibody - middle region (ARP35585_P050) in Human PANC1 cells using Western Blot
Human Lung; WB Suggested Anti-TPTE Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Lung; TPTE antibody - middle region (ARP37689_P050) in Human Lung cells using Western Blot
TA303136 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of HepG2 cell lysates with anti-TPTE antibody (1:200). This antibody identified a band of ~51kDa in HepG2 lysates.
WB Suggested Anti-TPTE Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Lung
WB Suggested Anti-TPTE Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: PANC1 cell lysate
WB Suggested Anti-TPTE Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysate
WB Suggested Anti-TPTE Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate
Human Pancreas
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP54819SU-N | TPTE antibody |
||||||
 |
Rabbit | Serum | Hu | C, E, ICC/IF, P, WB |
0.2 ml /
€430.00
|
||
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN206018 | TPTE - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206018BN | TPTE - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206018LP | TPTE - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN206018RB | TPTE - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP306018 | TPTE (transcript variant 2) |
||||
| Recombinant protein of human transmembrane phosphatase with tensin homology (TPTE), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY404597 | TPTE overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY404598 | TPTE overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. | ||
| LY430804 | TPTE overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC218792L1V | Lenti ORF particles, TPTE (Myc-DDK tagged) - Human transmembrane phosphatase with tensin homology (TPTE), transcript variant 3 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,080.00
|
|
| OriGene Technologies, Inc. | ||