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TRHR antibody

Principal name

TRHR antibody

Alternative names for TRHR antibody

TRH-R, Thyrotropin-releasing hormone receptor, Thyroliberin receptor

SwissProt ID

O46639 (Bovin), O93603 (Chick), P21761 (Mouse), P34981 (Human), Q01717 (Rat), Q28596 (Sheep)

Gene ID

7201 (TRHR), 25570 (Trhr)

Ncbi ID

NP_003292.1, NP_003292, 9606, ADR82960.1, NM_003301, NP_037179.1, NM_013047

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Mky (Monkey), Ms (Mouse), Op (Opossum), Rt (Rat), Chk (Chicken), Por (Porcine), African clawed frog, Chimp (Chimpanzee), Fi (Fish), Hst (Hamster), Tr (Turkey), Eq (Equine), Rb (Rabbit), Sh (Sheep), Xen (Xenopus), Bear, L (Lizard)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of TRHR antibody

Thyrotropin-releasing hormone (TRH), is a widely distributed tripeptide which acts as a hormone, a paracrine regulatory factor and a neurotransmitter/neuromodulator; it is expressed in many parts of the brain as well as in various peripheral tissues. In the hypothalamus, TRH is secreted from the paraventricular nucleus (VPN) by TRH neurons prior to a post-translatiol processing from its precursor, pro-TRH. A key player in the regulation of the hypothalamus-pituitary-thyroid (HPT) axis, TRH is released into the hypophyseal portal circulation of the median eminence and reaches the pituitary, where it binds to TRH receptors, thereby stimulates the secretion of tyrothropin (thyroid-stimulating hormone; TSH). TSH, in turn, affects the thyroid to produce T4 which is subsequently converted into its biologically-active alog, T3 - a negative mediator of TRH. In the tanycytes, ependymal cells of glial origin located in close proximity to the VPN, T4 is converted to T3 by the enzyme deiodise II (dio2) - a counterpart to deiodise III (dio3) which conversely ictivates the above mentioned thyroid hormones, thus so indirectly promotes the production of TRHs. TRH receptor 1 (TRH-R1), the only identified TRH receptor in humans, is a G protein-coupled receptor (GPCR) abundant in the anterior pituitary, neuroendocrine system, autonomic nervous system and the visceral regions of the brain. Like all members of GPCRs, TRH receptor has seven transmembrane domains, and extracellular N-terminus and an intracellular C-termil tail. TRH receptor couples Gq upon activation which in turn leads to the activation of phospholipase C. From a behavioral aspect, it was shown that TRH-coupled TRH-R1 increases cognitive arousal by directly exciting the sleep/wake system in the lateral hypothalamic area, and inhibits seizure activity in the hippocampus by facilitating GABA release, whereas its deficiency, as observed in mutant model mice, is linked to growth retardation, depression, and anxiety-like behavior.

General readings

Iwasaki, T., et al., (1996). J. Biol. Chem. 271 (36), 22183-22188.

Thyroid: Formalin-Fixed Paraffin-Embedded (FFPE)

AP08270PU-N

Immunofluorescence analysis of A549 cells, using TRHR Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COS7 cells, using TRHR Antibody. The lane on the right is treated with the synthesized peptide.

IHC analysis of human thyroid using TRHR polyclonal antibody ( Cat # PB0315 ) at 10 µg/ml.

PAB0315

Western blot analysis of human TRHR in human brain lysate in the 1 ) absence and 2 ) presence of immunizing peptide. Using TRHR polyclonal antibody ( Cat # PB0315 ) at 0.5 µg/ml.

PAB0315

Western blot (WB) analysis of TRH-R1 antibody (Cat.-No.: AP01441PU-N) in extracts from COS7 cells.

AP01441PU-N

Western blot analysis of rat brain (lanes 1 and 4), Jurkat cells (lanes 2 and 5) and MS1cells (lanes 3 and 6) lysates: 1-3. Anti-Thyrotropin-Releasing Hormone Receptor 1 antibody, (1:200). 4-6. Anti-Thyrotropin-Releasing Hormone Receptor 1 antibody, preincubated with the control peptide antigen.

TA329064

Expression of Thyrotropin-Releasing Hormone Receptor 1 in mouse MS1 cells. Immunocytochemical staining of mouse pancreatic islet endothelial (MS1) cells. Cells were stained using Anti-Thyrotropin-Releasing Hormone Receptor 1 antibody followed by goat-anti-rabbit-AlexaFluor-594 secondary antibody (red). Nuclear staining was demonstrated using DAPI as the counterstain (blue).

TA329064

Western blot analysis of extracts from COS-7 cells, using TRHR antibody.The lane on the right is treated with the synthesized peptide.

TA314641

Immunofluorescence analysis of A549 cells, using TRHR antibody.The picture on the right is treated with the synthesized peptide.

TA314641

Anti-TRH Receptor / TRHR antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340583

Anti-TRH Receptor / TRHR antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340583

Anti-TRH Receptor antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA340583

Figure 1. Immunohistochemical staining of brain (neurons and glia) using anti-TRHR antibody SP4630P.

TA340796

Immunohistochemical staining of Brain (Neurons and Glia) using anti- TRHR antibody SP4631P

TA340797

Immunohistochemical staining of formalin.fixed, paraffin-embedded human brain (neuron) after heat-induced antigen retrieval using anti-TRHR antibody cat. no. AP06817PU-N

TA340798

Western blot analysis on COS7 cell lysate using TRHR Antibody

TA347796

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA314641	TRHR antibody
	Rabbit polyclonal anti-TRHR antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, ICC/IF, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image
TA329064	TRHR antibody
	Rabbit polyclonal Anti-Thyrotropin-Releasing Hormone Receptor 1
	Rabbit		Purified	Hu, Ms, Rt	WB	0.2 ml / €585.00
						OriGene Technologies, Inc.
+1 additional image
TA340583	TRHR (C-term) antibody
	Rabbit Polyclonal Anti-TRHR Antibody (C-Terminus)
	Rabbit		Aff - Purified	Fi, Hst, Hu, Ms, Op, Rt, Tr	P	50 µg / €425.00
						OriGene Technologies, Inc.
TA340796	TRHR (3rd cytoplasm. dom.) antibody
	Rabbit Polyclonal Anti-TRHR Antibody (Cytoplasmic Domain)
	Rabbit		Aff - Purified	Bear, Bov, Can, Chk, Elephant, Eq, Hu, Ms, Platypus, Por, Rb, Rt, Sh, Tr, Xen	P	50 µg / €425.00
						OriGene Technologies, Inc.
TA340797	TRHR (C-term) antibody
	Rabbit Polyclonal Anti-TRHR Antibody (C-Terminus)
	Rabbit		Aff - Purified	Bear, Bov, Can, Elephant, Eq, Hst, Hu, Ms, Op, Rb, Rt, Sh	P	50 µg / €425.00
						OriGene Technologies, Inc.
TA340798	TRHR (C-term) antibody
	Rabbit Polyclonal Anti-TRHR Antibody (C-Terminus)
	Rabbit		Aff - Purified	Can, Chk, Elephant, Eq, Fi, Hu, L, Op, Por, Rb, Sh, Tr, Xen	P	50 µg / €425.00
						OriGene Technologies, Inc.
TA347796	TRHR antibody
	Rabbit Polyclonal Anti-TRHR Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 mg / €335.00
						OriGene Technologies, Inc.
AP08270PU-N	TRHR (350-400) antibody
	Rabbit	IgG	Aff - Purified	Bov, Can, Hu, Mky, Ms, Op, Rt	P, WB	50 µg / €425.00
						OriGene Technologies GmbH
AP01441PU-N	TRHR1 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	ICC/IF, WB	0.1 mg / €370.00
						OriGene Technologies GmbH

Lysates

Catalog No.
LY401138	TRHR overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR206508L2V	Lenti ORF particles, Trhr (GFP-tagged ORF) - Rat thyrotropin releasing hormone receptor (Trhr), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

RR206508L1V	Lenti ORF particles, Trhr (Myc-DDK-tagged ORF) - Rat thyrotropin releasing hormone receptor (Trhr), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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