TRIB1 antibody
Principal name
TRIB1 antibody
Alternative names for TRIB1 antibody
Tribbles homolog 1, TRB1, TRB-1, SKIP1, GIG2, GIG-2, C8FW
Ncbi ID
9606, NP_079471, NP_079471.1, NM_025195, NP_653132.1, NM_144549
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of TRIB1 antibody
- Kit Component:
- - KN318176G1, Trib1 gRNA vector 1 in pCas-Guide vector
- KN318176G2, Trib1 gRNA vector 2 in pCas-Guide vector
- KN318176D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
TRIB1 monoclonal antibody (M01), clone 4A10. Western Blot analysis of TRIB1 expression in human spleen.
Detection limit for recombinant GST tagged TRIB1 is approximately 0.1ng/ml as a capture antibody.
Western blot analysis of TRIB1 polyclonal antibody ( Cat # PAB3446 ) in T-47D cell line lysate ( 35 µg/lane ) . TRIB1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Western blot analysis of anti-TRIB1 Pab in T47D cell line lysate (35ug/lane). TRIB1(arrow) was detected using the purified Pab (1:60 dilution).
Human Lung Tissue; Rabbit Anti-TRIB1 Antibody . Catalog Number: ARP52412_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue . Observed Staining: Cytoplasmic in alveolar type I & II cells . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-TRIB1 Antibody (ARP52412_P050) ) in Human Lung Tissue using Immunohistochemistry
Human COLO205; Host: Rabbit . Target Name: TRIB1 . Sample Tissue: COLO205 . Antibody Dilution: 1.0ug/ml. TRIB1 is supported by BioGPS gene expression data to be expressed in COLO205.; TRIB1 antibody - middle region (ARP52412_P050) in Human COLO205 cells using Western Blot
Human Intestine; WB Suggested Anti-TRIB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Intestine; TRIB1 antibody - middle region (ARP52412_P050) in Human Intestine cells using Western Blot
Human HeLa; WB Suggested Anti-TRIB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate. TRIB1 is supported by BioGPS gene expression data to be expressed in HeLa.; TRIB1 antibody - C-terminal region (ARP52413_P050) in Human HeLa cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-TRIB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805274)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805274)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB1 (RC209219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB1 (RC209219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB1 (RC209219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB1.
(0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-TRIB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Intestine
Host: Rabbit; Target Name: TRIB1; Sample Tissue: COLO205; Antibody Dilution: 1.0 ug/mlTRIB1 is supported by BioGPS gene expression data to be expressed in COLO205
Rabbit Anti-TRIB1 Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue; Observed Staining: Cytoplasmic in alveolar type I & II cells; Primary Antibody Concentration: 1: 100; Secondary Antibody: Donkey anti-Rabbit-Cy3; Secondary Antibody Co
WB Suggested Anti-TRIB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Hela cell lysateTRIB1 is supported by BioGPS gene expression data to be expressed in HeLa
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311573 | TRIB1 (304-317) antibody |
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| Goat Anti-Trib1 (mouse) (aa304-317) Antibody | |||||||
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Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA351856 | TRIB1 antibody |
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| Rabbit Polyclonal Anti-TRIB1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||