TRIM35 antibody
Principal name
TRIM35 antibody
Alternative names for TRIM35 antibody
HLS5, KIAA1098, Tripartite motif-containing protein 35, Hemopoietic lineage switch protein 5
Ncbi ID
9606, NP_055881, NP_741983.2, NP_741983, NM_171982, NP_084255.2, NM_029979, NM_001025142
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))
Background of TRIM35 antibody
- Kit Component:
- - KN209628G1, TRIM35 gRNA vector 1 in pCas-Guide vector
- KN209628G2, TRIM35 gRNA vector 2 in pCas-Guide vector
- KN209628D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Lalonde J.-P., et al., (2004) J. Biol. Chem. 279:8181-8189
Immunoperoxidase of monoclonal antibody to TRIM35 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged TRIM35 is approximately 0.03ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-TRIM35 Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; TRIM35 antibody - C-terminal region (ARP39165_T100) in Human HepG2 cells using Western Blot
Human HepG2; WB Suggested Anti-TRIM35 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; TRIM35 antibody - C-terminal region (ARP34872_P050) in Human HepG2 cells using Western Blot
Human Muscle; WB Suggested Anti-TRIM35 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Muscle; TRIM35 antibody - middle region (ARP39970_P050) in Human Muscle cells using Western Blot
Human Small Intestine; Immunohistochemistry with Human Small Intestine tissue at an antibody concentration of 5.0ug/ml using anti-TRIM35 antibody (ARP34445_P050); TRIM35 antibody - middle region (ARP34445_P050) in Human Small Intestine cells using Immunohistochemistry
Human HeLa; WB Suggested Anti-TRIM35 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; TRIM35 antibody - middle region (ARP34445_P050) in Human HeLa cells using Western Blot
(1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Host: Rabbit; Target Name: Trim35; Sample Tissue: Mouse Kidney lysates; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-TRIM35 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate
Immunohistochemistry with Human Small Intestine tissue at an antibody concentration of 5.0 ug/ml using anti-TRIM35 antibody
WB Suggested Anti-TRIM35 Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysate
WB Suggested Anti-TRIM35 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Muscle
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A375 cells, human fetal muscle tissue, Primary antibody: (TRIM35 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (TRIM35 Antibody) at dilution 1/35, on the right is treated with synthetic peptide. (Original magnification: ×200)
Gel: 8%SDS-PAGE,Lysate: 40 ug,Lane 1-4: 293T cells, HepG2 cells, K562 cells, Hela cells,Primary antibody: (TRIM35 Antibody) at dilution 1/800 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 15 seconds
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311587 | TRIM35 (N-term) antibody |
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| Goat Anti-TRIM35 / MAIR Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350504 | TRIM35 antibody |
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| Rabbit Polyclonal Anti-TRIM35 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351812 | TRIM35 antibody |
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| Rabbit Polyclonal Anti-TRIM35 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY406788 | TRIM35 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214367L2V | Lenti ORF particles, Trim35 (GFP-tagged ORF) - Rat tripartite motif-containing 35 (Trim35), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,210.00
|
|
| OriGene Technologies, Inc. | ||
| RR214367L1V | Lenti ORF particles, Trim35 (Myc-DDK-tagged ORF) - Rat tripartite motif-containing 35 (Trim35), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,080.00
|
|
| OriGene Technologies, Inc. | ||