TRIM63 / MURF1 / RNF28 antibody
Principal name
TRIM63 / MURF1 / RNF28 antibody
Alternative names for TRIM63 / MURF1 / RNF28 antibody
MURF-1, IRF, SMRZ, RING finger protein 28, E3 ubiquitin-protein ligase TRIM63, Tripartite motif-containing protein 63, Muscle-specific RING finger protein 1, Iris RING finger protein, Striated muscle RING zinc finger protein
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of TRIM63 / MURF1 / RNF28 antibody
- Kit Component:
- - KN210267G1, TRIM63 gRNA vector 1 in pCas-Guide vector
- KN210267G2, TRIM63 gRNA vector 2 in pCas-Guide vector
- KN210267D, donor vector containing left and right homologous arms and RFP-BSD functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Urso ML, Chen YW, Scrimgeour AG, Lee PC, Lee KF, Clarkson PM. Alterations in mRNA expression and protein products following spinal cord injury in humans.
J Physiol. 2007 Mar 15;579(Pt 3):877-92.PMID: 17218363
2. de Palma L, Marinelli M, Pavan M, Orazi A.
Ubiquitin ligases MuRF1 and MAFbx in human skeletal muscle atrophy. Joint Bone Spine. 2008 Jan;75(1):53-7. PMID: 17977773
3. Salanova M, Schiffl G, Püttmann B, Schoser BG, Blottner D. Molecular biomarkers monitoring human skeletal muscle fibres and microvasculature following long-term bed rest with and without countermeasures. J Anat. 2008 Mar;212(3):306-18. PMID: 18221329
Immunofluorescence of monoclonal antibody to TRIM63 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of TRIM63 expression in transfected 293T cell line by TRIM63 monoclonal antibody (M01), clone 6G6.Lane 1: TRIM63 transfected lysate(40.2 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of TRIM63 over-expressed 293 cell line, cotransfected with TRIM63 Validated Chimera RNAi ( Cat # H00084676-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM63 monoclonal antibody (M01), clone 6G6 (Cat # H00084676-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Detection limit for recombinant GST tagged TRIM63 is approximately 0.1ng/ml as a capture antibody.
TRIM63 polyclonal antibody (A01), Lot # 051219JC01 Western Blot analysis of TRIM63 expression in HepG2 ( Cat # L019V1 ).
TRIM63 polyclonal antibody ( Cat # PAB7497 ) ( 1.25 µg/ml ) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot analysis of TRIM63 expression in transfected 293T cell line (H00084676-T01) by TRIM63 MaxPab polyclonal antibody.Lane 1: TRIM63 transfected lysate(38.83 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of TRIM63 expression in transfected 293T cell line (H00084676-T02) by TRIM63 MaxPab polyclonal antibody.Lane 1: TRIM63 transfected lysate(40.20 KDa).Lane 2: Non-transfected lysate.
TRIM63 MaxPab rabbit polyclonal antibody. Western Blot analysis of TRIM63 expression in NIH/3T3.
TRIM63 MaxPab rabbit polyclonal antibody. Western Blot analysis of TRIM63 expression in NIH/3T3.
Immunoprecipitation of TRIM63 transfected lysate using anti-TRIM63 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TRIM63 MaxPab mouse polyclonal antibody (B01) (H00084676-B01).
Human THP-1; WB Suggested Anti-TRIM63 Antibody Titration: 0.2-1 ug/ml. Positive Control: THP-1 cell lysate; TRIM63 antibody - middle region (ARP43365_P050) in Human THP-1 cells using Western Blot
TA302858 (1.25µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Sample(30 ug whole cell lysate). A:Hep G2 . 10% SDS PAGE. TA308746 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Breast ca, using MURF1(TA308746) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MURF1 (TA308746) antibody at 1:200 dilution.
Predicted band size: 40 kDa . Positive control: NIH/3T3 cell and Human fetal muscle tissue lysate. Recommended dilution: 1/1000-5000
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TRIM63 antibody at dilution 1/70, on the right is treated with the synthetic peptide. (Original magnification: x200)
Predicted band size: 40 kDa . Positive control: Human fetal muscle tissue lysate. Recommended dilution: 1/1000-5000
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TRIM63 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification: x200)
WB Suggested Anti-TRIM63 Antibody Titration: 0.2-1 ug/ml; Positive Control: THP-1 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302858 | TRIM63 / MURF1 / RNF28 (N-term) antibody |
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| Goat Polyclonal Antibody against TRIM63 | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu | E, P |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA308746 | TRIM63 / MURF1 / RNF28 antibody |
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| Rabbit Polyclonal antibody to MURF1 (tripartite motif-containing 63) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA322134 | TRIM63 / MURF1 / RNF28 antibody |
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| Anti-TRIM63 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA322135 | TRIM63 / MURF1 / RNF28 antibody |
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| Anti-TRIM63 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY410019 | TRIM63 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR202382L2V | Lenti ORF particles, Trim63 (GFP-tagged ORF) - Rat tripartite motif-containing 63 (Trim63), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR202382L1V | Lenti ORF particles, Trim63 (Myc-DDK-tagged ORF) - Rat tripartite motif-containing 63 (Trim63), 200 uL, >10^7 TU/mL |
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|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
