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TRPA1 antibody

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Principal name

TRPA1 antibody

Alternative names for TRPA1 antibody

ANKTM1, Transient receptor potential cation channel subfamily A member 1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120

SwissProt ID

O75762 (Human), Q18297 (Caeel), Q6RI86 (Rat), Q7Z020 (Drome), Q8BLA8 (Mouse)

Gene ID

8989 (TRPA1), 277328 (Trpa1), 312896

Ncbi ID

NP_015628.2, 10090, NP_808449.1, NM_007332, NM_207608, NM_177781

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of TRPA1 antibody

The TRPA family is comprised of only one mammalian member, the TRPA1 (formerly med ANKTM1). TRPA1 is expressed in peripheral sensory neurons, where it is suggested to contribute to the detection of painful stimuli. Origilly it was thought that TRPA channels sensed painfully cold temperatures, but a more conservative description is that TRPA1 is sensitive to membrane/cytoskeletal perturbations by cold and perhaps stretch. In addition, it is sensitive to pungent tural compounds present in cinmon oil, mustard oil, and wintergreen oil. TRPA1 is also expressed in hair cells, where its role in sensing mechanical forces is still unclear and controversial. TRPA1 has similar structure as all TRP ion channel members, six transmembrane domains, intracellular N-and C-terminus. However, its N-terminus domain possesses 17 ankyrin repeats that might indicate its potential role as a mechanosensor. TRPA1 is expressed in nociceptive neurons expressing TRPV1 as well and might serve as a marker for poly-modal nociceptors.

ARP35205_P050 - TRPA1
Mouse; . Sample Type :. Section of TRPM8-GFP transgenic mouse trigeminal ganglia. Primary Antibody Dilution :. 1:250. Secondary Antibody :. Goat Anti-Rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. TRPA1: Red TRPM8-GFP:: Green. Gene Name :. TRPA1. Submitted by :. Dr. Daniel McCoy, USC; TRPA1 antibody - middle region (ARP35205_P050) in Mouse cells using Immunohistochemistry
ARP35205_P050 - TRPA1
rat sensory ganglia; Immunohistochemistry with rat sensory ganglia tissue; TRPA1 antibody - middle region (ARP35205_P050) in rat sensory ganglia cells using Immunohistochemistry
ARP35205_P050 - TRPA1
Human Stomach; WB Suggested Anti-TRPA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Stomach; TRPA1 antibody - middle region (ARP35205_P050) in Human Stomach cells using Western Blot
H00008989-D01P - TRPA1
Western Blot analysis of TRPA1 expression in transfected 293T cell line (H00008989-T02) by TRPA1 MaxPab polyclonal antibody.Lane 1: TRPA1 transfected lysate(123.09 KDa).Lane 2: Non-transfected lysate.
PAB11992 - TRPA1
Western blot analysis of TRPA1 in human brain membrane fraction with TRPA1 polyclonal antibody at 2 µg/ml ( Cat# PAB11992 ).
PAB11992 - TRPA1
Immunohistochemical staining of TRPA1 on mouse intestine with TRPA1 polyclonal antibody ( Cat # PAB11992 ).
PAB8504 - TRPA1
Western blot analysis of HEK 293T cells overexpressing rat Trpa1, using Trpa1 polyclonal antibody ( Cat # PAB8504 ) .
PAB8504 - TRPA1
Immunoprecipitation analysis of HEK 293T cells overexpressing rat Trpa1, using Trpa1 polyclonal antibody ( Cat # PAB8504 ) .
TA309920 - TRPA1
Detection of TRPA1 in human brain membrane fraction using 2ug/ml of anti-TRPA1.
TA309920 - TRPA1
Staining TRPA1 in mouse intestine using anti-TRPA1.
TA322424 - TRPA1
Predicted cell location: Cytoplasm . Positive control: Human gastric and lung cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using TRPA1 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x 200)
TA322424 - TRPA1
Predicted cell location: Cytoplasm . Positive control: Human gastric and lung cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using TRPA1 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA328744 - TRPA1
Western blot analysis of rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates: 1,3,4. Anti-TRPA1 (extracellular) antibody, (1:200). 2,5,6. Anti-TRPA1 (extracellular) antibody, preincubated with the control peptide antigen.
TA328744 - TRPA1
Expression of TRPA1 in mouse DRG. Immunohistochemical staining of mouse dorsal root ganglion (DRG) frozen sections using Anti-TRPA1 (extracellular) antibody. A. TRPA1 (green) was distributed in patches (arrows). B. Neurons containing neurofilament 200 (red) also were distributed in patches. C. Confocal merge of TRPA1 and neurofilament 200 demonstrate partial overlap of these patches (arrows).
TA328744 - TRPA1
Expression of TRPA1 in rat PC12 cells. Immunocytochemical staining of intact living rat PC12 cells. A. Extracellular staining of cells using Anti-TRPA1 (extracellular) antibody, (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merged image of A and B.
TA328744 - TRPA1
Immunoprecipitation of PC-12 lysates: 1. PC-12 lysates. 2. PC-12 lysates + Anti-TRPA1 (extracellular) antibody (6.5 µg) + protein A beads. 3. Anti-TRPA1 (extracellular) antibody + protein A beads.
TA338564 - TRPA1
WB Suggested Anti-TRPA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Stomach
TA338564 - TRPA1
Immunohistochemistry with rat sensory ganglia tissue
TA341127 - TRPA1
Anti-TRPA1 antibody IHC of human ganglion cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA341128 - TRPA1
Anti-TRPA1 antibody IHC of human spinal cord, dorsal root ganglion. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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10 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

TRPA1 antibody
  Rabbit Polyclonal Anti-TRPA1 (extracellular)  
Western blot analysis of rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates: 1,3,4. Anti-TRPA1 (extracellular) antibody, (1:200). 2,5,6. Anti-TRPA1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €585.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

TRPA1 antibody
  Rabbit Polyclonal TRPA1 Antibody  
Detection of TRPA1 in human brain membrane fraction using 2ug/ml of anti-TRPA1. Rabbit Purified Hu, Ms C, WB
0.1 ml / €475.00
  OriGene Technologies, Inc.
+1 additional image

TRPA1 antibody
  Anti-TRPA1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm . Positive control: Human gastric and lung cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using TRPA1 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

TRPA1 antibody
  Rabbit Polyclonal Anti-TRPA1 Antibody (Internal)  
Anti-TRPA1 antibody IHC of human ganglion cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €425.00
  OriGene Technologies, Inc.

TRPA1 antibody
  Rabbit Polyclonal Anti-TRPA1 Antibody (Internal)  
TRPA1 Rabbit Aff - Purified Hu, Mky P
50 µg / €425.00
  OriGene Technologies, Inc.

TRPA1 antibody
TRPA1 staining of rat DRG using FITC-conjugated donkey anti-rabbit  secondary antibodies (green color) and propidium iodide as a counterstain. Rabbit Serum Rt C
0.1 ml / €290.00
  Neuromics Antibodies
+1 additional image

TRPA1 (N-term) antibody
Western blot of TRPA1 in human brain membrane fraction. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €370.00
  Neuromics Antibodies

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, TRPA1 (mGFP-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1), 200 uL, >10^7 TU/mL
Lenti ORF particles, TRPA1 (mGFP-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1), 200 uL, >10^7 TU/mL  
200 µl / €2,380.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, TRPA1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1), 200 uL, >10^7 TU/mL
Lenti ORF particles, TRPA1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily A, member 1 (TRPA1), 200 uL, >10^7 TU/mL  
200 µl / €2,140.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Trpa1 (GFP-tagged ORF) - Rat transient receptor potential cation channel, subfamily A, member 1 (Trpa1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Trpa1 (GFP-tagged ORF) - Rat transient receptor potential cation channel, subfamily A, member 1 (Trpa1), 200 uL, >10^7 TU/mL  
200 µl / €2,450.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. Human Lenti ORF Particle (2)
  2. Primary Antibody (7)
  3. Rat Lenti ORF Particle (1)
Clonality
  1. Polyclonal (7)
Target
  1. TRPA1 (7)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (6)
  2. Mouse (Ms) (4)
  3. Rat (Rt) (3)
  4. Monkey (Mky) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Frozen sections (C) (2)
  3. Paraffin Sections (P) (2)
  4. Enzyme Immunoassay (E) (1)
Presentation
  1. Purified (4)
  2. Aff - Purified (2)
  3. Serum (1)
Host
  1. Rabbit (7)
Images
  1. 1 image (6)
  2. 3+ images (1)
Images: Applications
  1. IHC (4)
  2. Western blot / Immunoblot (WB) (2)
  3. Immunofluorescence (IF) (1)
  4. Immunoprecipitation (IP) (1)
Manufacturer
  1. OriGene Technologies (8)
  2. Neuromics Antibodies (2)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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