TSC22D1 antibody
Principal name
TSC22D1 antibody
Alternative names for TSC22D1 antibody
TSC22 domain family protein 1, TSC22, KIAA1994, TGFB1I4, Transforming growth factor beta-1-induced transcript 4 protein, Regulatory protein TSC-22, TGFB-stimulated clone 22 homolog, Cerebral protein 2
Ncbi ID
9606, NP_006013.1, NP_904358, NP_006013, NM_009366, NM_001109912, NM_013043, NM_207652, NM_006022, NM_183422
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)
Background of TSC22D1 antibody
- Kit Component:
- - KN223210G1, TSC22D1 gRNA vector 1 in pCas-Guide vector
- KN223210G2, TSC22D1 gRNA vector 2 in pCas-Guide vector
- KN223210D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Doi,Y., (2008) Oncol. Rep. 19 (3), 609-616
Immunofluorescence of monoclonal antibody to TSC22D1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of TSC22D1 expression in transfected 293T cell line by TSC22D1 monoclonal antibody (M01), clone 1G7.Lane 1: TSC22D1 transfected lysate(15.7 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged TSC22D1 is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged TSC22D1 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to TSC22D1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of TSC22D1 expression in transfected 293T cell line (H00008848-T01) by TSC22D1 MaxPab polyclonal antibody.Lane 1: TSC22D1 transfected lysate(15.84 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry (IHC) analyzes of TSC-22 antibody (Cat.-No.: AP01239PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of TSC-22 antibody (Cat.-No.: AP01239PU-N) in extracts from mouse liver.
Western Blot analysis of TSC22D1 expression in transfected 293T cell line (H00008848-T02) by TSC22D1 MaxPab polyclonal antibody.Lane 1: TSC22D1 transfected lysate(15.70 KDa).Lane 2: Non-transfected lysate.
TSC22D1 MaxPab rabbit polyclonal antibody. Western Blot analysis of TSC22D1 expression in Hela NE.
Human MCF-7; WB Suggested Anti-TSC22D1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: MCF7 cell lysate. TSC22D1 is supported by BioGPS gene expression data to be expressed in MCF7.; TSC22D1 antibody - middle region (ARP34697_P050) in Human MCF-7 cells using Western Blot
TSC22D1 MaxPab rabbit polyclonal antibody. Western Blot analysis of TSC22D1 expression in Hela NE.
Immunoprecipitation of TSC22D1 transfected lysate using anti-TSC22D1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TSC22D1 purified MaxPab mouse polyclonal antibody (B01P) (H00008848-B01P).
Human ACHN; WB Suggested Anti-TSC22D1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate; TSC22D1 antibody - middle region (ARP38726_P050) in Human ACHN cells using Western Blot
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of HepG2 cells, using TSC22D1 Antibody Cat.-No AP32264PU-N. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from mouse liver/rat liver cells, using TSC22D1 Antibody Cat.-No AP32264PU-N. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of TSC22 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of TSC22 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of TSC22 expression in MCF7 (A); HeLa (B); mouse liver (C); rat liver (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505047)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505047)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D1 (RC223210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505048)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505048)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505048)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505048)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505048)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505048)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505048)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505048)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-TSC22D1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D1 (RC223210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D1.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505058)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505058)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505058)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505058)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505058)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505058)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505058)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D1 (RC223210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D1.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505060)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505060)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D1 (RC223210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505061)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505061)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505061)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505061)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D1 (RC223210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D1.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505062)
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-TSC22D1 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D1 (RC223210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D1.
Flow cytometric Analysis of Hela cells, using anti-TSC22D1 antibody(TA505062),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-TSC22D1 antibody(TA505062),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505063)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-TSC22D1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D1 (RC223210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505064)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505064)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505064)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505064)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TSC22D1 (RC223210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D1.
Western blot analysis of extracts from mouse liver cells and rat liver cells, using TSC22D1 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using TSC22D1 antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using TSC22D1 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-TSC22D1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: MCF7 cell lysateTSC22D1 is supported by BioGPS gene expression data to be expressed in MCF7
WB Suggested Anti-TSC22D1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: ACHN cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA312827 | TSC22D1 antibody |
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| Rabbit polyclonal anti-TSC22D1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP01239PU-N | TSC22D1 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP32264PU-N | TSC22D1 (N-term) antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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