TSG101 antibody
Principal name
TSG101 antibody
Alternative names for TSG101 antibody
Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101
Ncbi ID
9606, NP_006283, NP_068684, 5874, NP_006283.1, ENSP00000251968, NM_006292, NP_068684.1, NM_021884, NM_181628
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of TSG101 antibody
TSG101 belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. TSG101 contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. TSG101 may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of TSG101 appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in TSG101 gene occur in high frequency in breast cancer. The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
General readings
Vardhana S., et al. (2010) Immunity. 32(4):531-40.
Popov S., et al. (2009) J Virol. 83(14):7185-93.
Immunoperoxidase of monoclonal antibody to TSG101 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml]
Immunofluorescence of monoclonal antibody to TSG101 on HeLa cell. [antibody concentration 10 ug/ml]
Western blot analysis of TSG101 over-expressed 293 cell line, cotransfected with TSG101 Validated Chimera RNAi ( Cat # H00007251-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TSG101 monoclonal antibody (M01), clone 5B7 (Cat # H00007251-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of TSG101 expression in transfected 293T cell line by TSG101 monoclonal antibody (M01), clone 5B7.Lane 1: TSG101 transfected lysate(43.9 KDa).Lane 2: Non-transfected lysate.
TSG101 monoclonal antibody (M01), clone 5B7 Western Blot analysis of TSG101 expression in K-562 ( Cat # L009V1 ).
Detection limit for recombinant GST tagged TSG101 is approximately 0.03ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-TSG101 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. TSG101 is supported by BioGPS gene expression data to be expressed in HepG2.; TSG101 antibody - C-terminal region (ARP38773_T100) in Human HepG2 cells using Western Blot
Mouse Kidney; Rabbit Anti-Tsg101 Antibody. Catalog Number: ARP37310. Paraffin Embedded Tissue: Mouse Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; TSG101 antibody - middle region (ARP37310_T100) in Mouse Kidney cells using Immunohistochemistry
mouse fibroblast; . Sample Type:.  mouse fibroblast lusate (10ug). Primary Dilution:.  1:1000 (1% BSA). Secondary Dilution:.  1:2000 (5% milk). Image Submitted By: . . Anonymous researcher . . See Customer Feedback tab for detailed information.. .; TSG101 antibody - middle region (ARP37310_T100) in mouse fibroblast cells using Western Blot
Mouse NIH-3T3; WB Suggested Anti-TSG101 Antibody Titration: 1.25ug/ml. Positive Control: NIH/3T3 cell lysate; TSG101 antibody - middle region (ARP37310_T100) in Mouse NIH-3T3 cells using Western Blot
Mouse brain; WB Suggested Anti-TSG101 Antibody . . Positive Control: Lane 1: 30ug mouse brain lysate Lane 2: 30ug mouse brain lysate Lane 3: 30ug mouse brain lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti-rabbit-HRP . Secondry Antibody Dilution : 1:50,000. Submitted by: Teresa Gunn, McLaughin Research Institute; TSG101 antibody - middle region (ARP37310_T100) in Mouse brain cells using Western Blot
Mouse NIH-3T3; WB Suggested Anti-TSG101 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: NIH/3T3 cell lysate; TSG101 antibody - middle region (ARP37911_T100) in Mouse NIH-3T3 cells using Western Blot
The TSG101 polyclonal antibody ( Cat # PAB1758 ) is used in Western blot to detect TSG101 in ZR-75-1 cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TSG101 polyclonal antibody ( Cat # PAB1759 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The TSG101 polyclonal antibody ( Cat # PAB1759 ) is used in Western blot to detect TSG101 in mouse kidney tissue lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western Blot Analysis of anti-TSG101 (N-term) Pab (Cat.#AP12055PU-N) to detect TSG101 in mouse kidney tissue lysate.
Western Blot Analysis of anti-TSG101 (C-term) Pab (Cat.#AP12056PU-N) to detect TSG101 in ZR75-1 cell lysate.
Immunoprecipitation of TSG101 transfected lysate using anti-TSG101 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TSG101 MaxPab mouse polyclonal antibody (B01) (H00007251-B01).
Recombinant human TSG101, 1-145 aa, His-tagged: 15% SDS-PAGE (3 ug)
Immunohistochemistry (IHC) analyzes of Tsg 101 antibody (Cat.-No.: AP20455PU-N) in paraffin-embedded human brain tissue.
Western blot (WB) analysis of Tsg 101 antibody (Cat.-No.: AP20455PU-N) in extracts from HEK293A cells.
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to TSG101 on HeLa cell. [antibody concentration 10 µg/ml]
TSG101 monoclonal antibody clone 5B7 Western Blot analysis of TSG101 expression in K-562.
Detection limit for recombinant GST tagged TSG101 is approximately 0.03ng/ml as a capture antibody.
Western blot analysis of extracts from COS-7 cells, using TS101 antibody.The lane on the right is treated with the synthesized peptide.
Sample Type: mouse fibroblast lusate (10 ug); Primary Dilution: 1:1000 (1% BSA); Secondary Dilution: 1:2000 (5% milk); Image Submitted By: Anonymous researcher See Customer Feedback tab for detailed information.
WB Suggested Anti-TSG101 Antibody Titration: 1.25ug/ml; Positive Control: NIH/3T3 cell lysate
WB Suggested Anti-TSG101 Antibody ; Positive Control: Lane 1: 30ug mouse brain lysate Lane 2: 30ug mouse brain lysate Lane 3: 30ug mouse brain lysate; Primary Antibody Dilution : 1:1000; Secondary Antibody : Goat anti-rabbit-HRP; Secondry Antibody Dilutio
Rabbit Anti-Tsg101 Antibody; Paraffin Embedded Tissue: Mouse Kidney; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Host: Rabbit; Target Name: TSG101; Sample Tissue: Thymus tumor lysates; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-TSG101 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate; TSG101 is supported by BioGPS gene expression data to be expressed in HepG2
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM31014PU-N | TSG101 (201-281) antibody |
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Mouse | IgG2a | 5B7 | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€475.00
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| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA312310 | TSG101 antibody |
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| Rabbit polyclonal anti-TSG101 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP12055PU-N | TSG101 (N-term) antibody |
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Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP12056PU-N | TSG101 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
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|
| OriGene Technologies GmbH | |||||||
| AP20455PU-N | TSG101 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€370.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP710086 | TSG101 (full length, C-term DDK tag) |
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| Recombinant protein of human human tumor susceptibility gene 101 (TSG101), full length, with C-terminal DDK tag, expressed in sf9 cells | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
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|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206104L2V | Lenti ORF particles, Tsg101 (GFP-tagged) - Mouse tumor susceptibility gene 101 (Tsg101), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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|
| OriGene Technologies, Inc. | ||
| MR206104L1V | Lenti ORF particles, Tsg101 (Myc-DDK-tagged) - Mouse tumor susceptibility gene 101 (Tsg101), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR215604L2V | Lenti ORF particles, Tsg101 (GFP-tagged ORF) - Rat tumor susceptibility gene 101 (Tsg101), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR215604L1V | Lenti ORF particles, Tsg101 (Myc-DDK-tagged ORF) - Rat tumor susceptibility gene 101 (Tsg101), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||