TUFM antibody
Principal name
TUFM antibody
Alternative names for TUFM antibody
EF-Tu, Elongation factor Tu mitochondrial
Ncbi ID
9606, NP_003312, NP_003312.3, NP_001157185.1, NM_172745, NM_001106295, NM_003321
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of TUFM antibody
- Kit Component:
- - KN318483G1, Tufm gRNA vector 1 in pCas-Guide vector
- KN318483G2, Tufm gRNA vector 2 in pCas-Guide vector
- KN318483D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Valente,L., (2007) Am. J. Hum. Genet. 80 (1), 44-58
Detection limit for recombinant GST tagged TUFM is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to TUFM on HeLa cell. [antibody concentration 10 ug/ml]
TUFM MaxPab polyclonal antibody. Western Blot analysis of TUFM expression in rat brain.
Western Blot analysis of TUFM expression in transfected 293T cell line (H00007284-T01) by TUFM MaxPab polyclonal antibody.Lane 1: TUFM transfected lysate(50.05 KDa).Lane 2: Non-transfected lysate.
TUFM MaxPab polyclonal antibody. Western Blot analysis of TUFM expression in HeLa.
Western Blot analysis of TUFM expression in transfected 293T cell line (H00007284-T01) by TUFM MaxPab polyclonal antibody.Lane 1: TUFM transfected lysate(49.90 KDa).Lane 2: Non-transfected lysate.
Hum. Fetal Lung; Host:Rabbit. Target Name:TUFM. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; TUFM antibody - middle region (ARP58546_P050) in Hum. Fetal Lung cells using Western Blot
Human HeLa; WB Suggested Anti-TUFM Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate. TUFM is supported by BioGPS gene expression data to be expressed in HeLa.; TUFM antibody - middle region (ARP58546_P050) in Human HeLa cells using Western Blot
Western blot analysis of TUFM Antibody (N-term) (AP17813PU-N) in Ramos cell line lysates (35ug/lane). TUFM (arrow) was detected using the purified Pab.
Western Blot analysis of TUFM expression in transfected 293T cell line (H00007284-T01) by TUFM MaxPab polyclonal antibody.Lane 1: TUFM transfected lysate(49.90 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of TUFM transfected lysate using anti-TUFM MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TUFM purified MaxPab mouse polyclonal antibody (B01P) (H00007284-B01P).
Western blot analysis of TUFM (arrow) using rabbit polyclonal TUFM Antibody (C-term) (AP54422PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TUFM gene.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using TUFM antibody.The picture on the right is treated with the synthesized peptide.
Host:Rabbit; Target Name:TUFM; Sample Tissue:Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
WB Suggested Anti-TUFM Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Hela cell lysateTUFM is supported by BioGPS gene expression data to be expressed in HeLa
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314653 | TUFM antibody |
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| Rabbit polyclonal anti-TUFM antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP17813PU-N | TUFM (N-term) antibody |
||||||
 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
|||
| OriGene Technologies GmbH | |||||||
| AP54422PU-N | TUFM (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR217099L2V | Lenti ORF particles, Tufm (GFP-tagged) - Mouse Tu translation elongation factor, mitochondrial (Tufm), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR217099L1V | Lenti ORF particles, Tufm (Myc-DDK-tagged) - Mouse Tu translation elongation factor, mitochondrial (Tufm), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,000.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR214279L2V | Lenti ORF particles, Tufm (GFP-tagged ORF) - Rat Tu translation elongation factor, mitochondrial (Tufm), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,120.00
|
|
| OriGene Technologies, Inc. | ||
| RR214279L1V | Lenti ORF particles, Tufm (Myc-DDK-tagged ORF) - Rat Tu translation elongation factor, mitochondrial (Tufm), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,000.00
|
|
| OriGene Technologies, Inc. | ||