UBA5 / UBE1DC1 antibody
Principal name
UBA5 / UBE1DC1 antibody
Alternative names for UBA5 / UBE1DC1 antibody
Ubiquitin-activating enzyme 5, UFM1-activating enzyme, ThiFP1, Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme E1 domain-containing protein 1
Ncbi ID
9606, NP_938143, NP_079094.1, NP_079094, NM_198329, NP_079968.2, NM_001009669, NM_024818
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of UBA5 / UBE1DC1 antibody
- Kit Component:
- - KN318546G1, Uba5 gRNA vector 1 in pCas-Guide vector
- KN318546G2, Uba5 gRNA vector 2 in pCas-Guide vector
- KN318546D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Komatsu M., et al. (2004) EMBO J. 23:1977-1986Zheng M., et al. (2008) J. Cell. Biochem. 104:2324-2334
UBE1DC1 MaxPab polyclonal antibody. Western Blot analysis of UBE1DC1 expression in human colon.
Western Blot analysis of UBA5 expression in transfected 293T cell line (H00079876-T01) by UBA5 MaxPab polyclonal antibody.Lane 1: UBE1DC1 transfected lysate(44.44 KDa).Lane 2: Non-transfected lysate.
UBE1DC1 MaxPab polyclonal antibody. Western Blot analysis of UBE1DC1 expression in Jurkat.
Human Jurkat; WB Suggested Anti-UBA5 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; UBA5 antibody - middle region (ARP49880_P050) in Human Jurkat cells using Western Blot
Human Kidney, Tubules: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from HepG2/293 cells, using UBA5 Antibody. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of UBA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of UBA5 expression in HEK293T (A) whole cell lysates.
Western blot analysis of extracts from HepG2 cells and 293 cells, using UBA5 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-UBA5 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315811 | UBA5 / UBE1DC1 antibody |
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| Rabbit polyclonal anti-UBA5 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP31878PU-N | UBA5 / UBE1DC1 (C-term) antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302327 | UBA5 / UBE1DC1 (transcript variant 1) |
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| Recombinant protein of human ubiquitin-like modifier activating enzyme 5 (UBA5), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY405021 | UBA5 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY430737 | UBA5 overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC202327L2V | Lenti ORF particles, UBA5 (mGFP-tagged) - Human ubiquitin-like modifier activating enzyme 5 (UBA5), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212674L2V | Lenti ORF particles, Uba5 (GFP-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 5 (Uba5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR212674L1V | Lenti ORF particles, Uba5 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 5 (Uba5), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||