UBE2D2 antibody
Principal name
UBE2D2 antibody
Alternative names for UBE2D2 antibody
Ubiquitin-conjugating enzyme E2 D2, Ubiquitin-protein ligase D2, Ubiquitin carrier protein D2, UBC4, UBCH5B, E2(17)KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2
Ncbi ID
9606, NP_003330, NP_003330.1, NM_181838, NM_003339, NP_862821, NP_064296.1, NM_019912, NM_001037292
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)
Background of UBE2D2 antibody
- Kit Component:
- - KN318567G1, Ube2d2 gRNA vector 1 in pCas-Guide vector
- KN318567G2, Ube2d2 gRNA vector 2 in pCas-Guide vector
- KN318567D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Desforges,M., Am. J. Physiol., Cell Physiol. 290 (1), C305-C312 (2006)
Immunoperoxidase of monoclonal antibody to UBE2D2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
UBE2D2 monoclonal antibody (M02), clone 4A1. Western Blot analysis of UBE2D2 expression in PC-12 ( Cat # L012V1 ).
UBE2D2 monoclonal antibody (M02), clone 4A1 Western Blot analysis of UBE2D2 expression in NIH/3T3 ( Cat # L018V1 ).
UBE2D2 monoclonal antibody (M02), clone 4A1. Western Blot analysis of UBE2D2 expression in Raw 264.7 ( Cat # L024V1 ).
Detection limit for recombinant GST tagged UBE2D2 is approximately 3ng/ml as a capture antibody.
UBE2D2 polyclonal antibody (A01), Lot # SRP0060302QCS1 Western Blot analysis of UBE2D2 expression in HeLa ( Cat # L013V1 ).
Human Lung; UBE2D2 antibody - middle region (ARP43008_T100) in Human Lung cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-UBE2D2 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate; UBE2D2 antibody - middle region (ARP43008_T100) in Human Jurkat cells using Western Blot
recombinant protein; . Lanes: 1: 40ng HIS-UBE2D1 protein. 2: 40ng HIS-UBE2D2 protein. 3: 40ng HIS-UBE2D3 protein. 4: 40ng HIS-UBE2D4 protein. 5: 40ng HIS-UBE2E1 protein. 6: 40ng HIS-UBE2E2 protein. 7: 40ng HIS-UBE2E3 protein. 8: 40ng HIS-UBE2K protein. 9: 40ng HIS-UBE2L3 protein. 10: 40ng HIS-UBE2N protein. 11: 40ng HIS-UBE2V1 protein. 12: 40ng HIS-UBE2V2 protein.. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:50,000. Gene Name: UBE2D2. Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK..; UBE2D2 antibody - N-terminal region (ARP43007_P050) in recombinant protein cells using Western Blot
Human Brain; WB Suggested Anti-UBE2D2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; UBE2D2 antibody - N-terminal region (ARP43007_P050) in Human Brain cells using Western Blot
Human Liver; WB Suggested Anti-UBE2D2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Liver; UBE2D2 antibody - middle region (ARP43010_P050) in Human Liver cells using Western Blot
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
UBE2D2 monoclonal antibody clone 4A1 Western Blot analysis of UBE2D2 expression in NIH/3T3.
UBE2D2 monoclonal antibody clone 4A1. Western Blot analysis of UBE2D2 expression in PC-12.
Detection limit for recombinant GST tagged UBE2D2 is approximately 3ng/ml as a capture antibody.
Recombinant human UBE2D2, 1-147 aa, His-tagged
UBE2D2 Antibody (C-term) (AP54437PU-N) western blot analysis in 293 cell line lysates (35 µg/lane). This demonstrates the UBE2D2 antibody detected the UBE2D2 protein (arrow).
Western blot analysis of UBE2D2 expression in MCF7 (A); HepG2 (B); NIH3T3 (C); H9C2 (D) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2 (RC218795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2D2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2 (RC218795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2D2.
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806527)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806527)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2 (RC218795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2D2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806600)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2 (RC218795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2D2.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UBE2D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806601)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2 (RC218795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2D2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2 (RC218795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2D2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2D2 (RC218795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2D2.
Western blot analysis of extracts from HeLa cells, A549 cells and K562 cells, using UBE2D2 antibody.The lane on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314658 | UBE2D2 antibody |
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| Rabbit polyclonal anti-UBE2D2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP54437PU-N | UBE2D2 (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||