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UBE2D3 antibody

Principal name

UBE2D3 antibody

Alternative names for UBE2D3 antibody

UBC5C, UBCH5C, Ubiquitin-conjugating enzyme E2 D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin-conjugating enzyme E2-17 kDa 3, Ubiquitin-protein ligase D3

SwissProt ID

P61077 (Human), P61078 (Rat), P61079 (Mouse), Q3ZCF7 (Bovin)

Gene ID

7323 (UBE2D3), 66105 (Ube2d3), 81920

Ncbi ID

9606, NP_003331, NP_871622.1, NM_181893, NP_871615, NP_871618, NP_871616, NP_871622, NP_003331.1, NP_079632.1, NM_025356, NM_031237, NM_003340, NM_181886, NM_181889, NM_181890, NM_181892, NM_181888, NM_181891

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of UBE2D3 antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.

General readings

Gonen H., et al. (1999) J. Biol. Chem. 274:14823-14830Murata S., et al. (2001) EMBO Rep. 2:1133-1138

UBE2D3 monoclonal antibody (M01), clone 4C1-1E3 Western Blot analysis of UBE2D3 expression in Jurkat ( Cat # L017V1 ).

H00007323-M01

Western Blot analysis of UBE2D3 expression in transfected 293T cell line by UBE2D3 monoclonal antibody (M01), clone 4C1-1E3.Lane 1: UBE2D3 transfected lysate(16.7 KDa).Lane 2: Non-transfected lysate.

H00007323-M01

Detection limit for recombinant GST tagged UBE2D3 is approximately 1ng/ml as a capture antibody.

H00007323-M01

Hum. Fetal Lung; Host: Rabbit . Target Name: UBE2D3 . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; UBE2D3 antibody - N-terminal region (ARP43009_P050) in Hum. Fetal Lung cells using Western Blot

ARP43009_P050

Human HEK293T; Host: Rabbit . Target Name: UBE2D3 . Sample Tissue: 293T . Antibody Dilution: 1.0ug/ml. UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; UBE2D3 antibody - N-terminal region (ARP43009_P050) in Human HEK293T cells using Western Blot

ARP43009_P050

Hum. Fetal Liver; Host: Rabbit . Target Name: UBE2D3 . Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; UBE2D3 antibody - N-terminal region (ARP43009_P050) in Hum. Fetal Liver cells using Western Blot

ARP43009_P050

Human Jurkat; Host: Rabbit . Target Name: UBE2D3 . Sample Tissue: Jurkat . Antibody Dilution: 1.0ug/ml. UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in Jurkat.; UBE2D3 antibody - N-terminal region (ARP43009_P050) in Human Jurkat cells using Western Blot

ARP43009_P050

Human MCF-7; WB Suggested Anti-UBE2D3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: MCF7 cell lysate.. UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in MCF7.; UBE2D3 antibody - N-terminal region (ARP43009_P050) in Human MCF-7 cells using Western Blot

ARP43009_P050

Human MCF7; Host: Rabbit . Target Name: UBE2D3 . Sample Tissue: MCF7 . Antibody Dilution: 1.0ug/ml. UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in MCF7.; UBE2D3 antibody - N-terminal region (ARP43009_P050) in Human MCF7 cells using Western Blot

ARP43009_P050

Recombinant protein; . Lanes: 1: 40ng HIS-UBE2D1 protein. 2: 40ng HIS-UBE2D2 protein. 3: 40ng HIS-UBE2D3 protein. 4: 40ng HIS-UBE2D4 protein. 5: 40ng HIS-UBE2E1 protein. 6: 40ng HIS-UBE2E2 protein. 7: 40ng HIS-UBE2E3 protein. 8: 40ng HIS-UBE2K protein. 9: 40ng HIS-UBE2L3 protein. 10: 40ng HIS-UBE2N protein. 11: 40ng HIS-UBE2V1 protein. 12: 40ng HIS-UBE2V2 protein.. Primary Antibody Dilution: 1:500. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:50,000. Gene Name: UBE2D3. Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK..; UBE2D3 antibody - N-terminal region (ARP43009_P050) in Recombinant protein cells using Western Blot

ARP43009_P050

UBE2D3 Antibody (C-term) (AP54438PU-N) western blot analysis in Hela cell line lysates (35 µg/lane). This demonstrates the UBE2D3 antibody detected the UBE2D3 protein (arrow).