UBE2F antibody
Principal name
UBE2F antibody
Alternative names for UBE2F antibody
NEDD8-conjugating enzyme 2, NEDD8 carrier protein, NEDD8 protein ligase UBE2F, Ubiquitin-conjugating enzyme E2 F
Ncbi ID
9606, NP_542409, NP_542409.1, NM_080678, NM_026454, NM_001008381
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of UBE2F antibody
NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.
General readings
Huang D.T., et al. (2009) Mol. Cell 33:483-495Nandi, D. et al. (2006) Journal of biosciences 31 (1): 137-55.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP16957PU-N | UBE2F (C-term) antibody |
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 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Rt | E, P, WB |
0.1 mg /
€380.00
|
||
| OriGene Technologies GmbH | |||||||
 +1 additional image |
OriGene recommends this product for Western blotting. |
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| TA311564 | UBE2F (N-term) antibody |
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| Goat Anti-UBE2F / NCE2 (N Terminus) Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303942 | UBE2F |
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| Recombinant protein of human ubiquitin-conjugating enzyme E2F (putative) (UBE2F) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY409102 | UBE2F overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201724L2V | Lenti ORF particles, Ube2f (GFP-tagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR201724L1V | Lenti ORF particles, Ube2f (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR208623L2V | Lenti ORF particles, Ube2f (GFP-tagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR208623L1V | Lenti ORF particles, Ube2f (Myc-DDK-tagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||