UBE2G1 antibody
Principal name
UBE2G1 antibody
Alternative names for UBE2G1 antibody
Ubiquitin-conjugating enzyme E2 G1, Ubiquitin-protein ligase G1, Ubiquitin carrier protein G1, UBE2G, UBC7, E217K
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of UBE2G1 antibody
- Kit Component:
- - KN201159G1, UBE2G1 gRNA vector 1 in pCas-Guide vector
- KN201159G2, UBE2G1 gRNA vector 2 in pCas-Guide vector
- KN201159D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Shibata,E., et al. (2011) Mol. Cell. Biol. 31 (15), 3136-3145Hassink,G., et al. (2005) Biochem. J. 388 (PT 2), 647-655
Immunofluorescence of monoclonal antibody to UBE2G1 on HeLa cell. [antibody concentration 20 ug/ml]
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2G1 polyclonal antibody ( Cat # PAB3897 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The UBE2G1 polyclonal antibody ( Cat # PAB3897 ) is used in Western blot to detect UBE2G1 in mouse liver tissue lysate.
Western Blot analysis of UBE2G1 expression in transfected 293T cell line (H00007326-T01) by UBE2G1 MaxPab polyclonal antibody.Lane 1: UBE2G1 transfected lysate(18.7 KDa).Lane 2: Non-transfected lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-UBE2G1 Antibody (N-term) (Cat.#AP11982PU-N) in mouse heart tissue lysates (35ug/lane). UBE2G1 (arrow) was detected using the purified Pab.
Mouse Spleen; WB Suggested Anti-Ube2g1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Spleen; Ube2g1 antibody - N-terminal region (ARP43014_P050) in Mouse Spleen cells using Western Blot
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP11982PU-N | UBE2G1 (N-term) antibody |
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Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301159 | UBE2G1 |
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| Recombinant protein of human ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) (UBE2G1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| SA6047 | UBE2G1 |
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 |
Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.1 mg /
€250.00
|
| OriGene Technologies GmbH | |||||
| +1 additional image |
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| SA6047X | UBE2G1 |
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|
Human | Purified | > 95 % by SDS-PAGE | E. coli |
0.5 mg /
€600.00
|
| OriGene Technologies GmbH | |||||
| +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY418760 | UBE2G1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201429L1V | Lenti ORF particles, Ube2g1 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€740.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212717L2V | Lenti ORF particles, Ube2g1 (GFP-tagged ORF) - Rat ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans) (Ube2g1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR212717L1V | Lenti ORF particles, Ube2g1 (Myc-DDK-tagged ORF) - Rat ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans) (Ube2g1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||