UBE2S antibody
Principal name
UBE2S antibody
Alternative names for UBE2S antibody
Ubiquitin-conjugating enzyme E2 S, Ubiquitin-protein ligase S, Ubiquitin carrier protein S, E2-EPF5, E2EPF, OK/SW-cl.73, Ubiquitin-conjugating enzyme E2-24 kDa
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)
Background of UBE2S antibody
- Kit Component:
- - KN318590G1, Ube2s gRNA vector 1 in pCas-Guide vector
- KN318590G2, Ube2s gRNA vector 2 in pCas-Guide vector
- KN318590D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ye Y., et al. (2009) Nat Rev Mol Cell Biol. 10(11):755-64.
Windheim M., et al. (2008) Biochem J. 409(3):723-9.
Detection limit for recombinant GST tagged UBE2S is approximately 0.03ng/ml as a capture antibody.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2S polyclonal antibody ( Cat # PAB1701 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of UBE2S ( arrow ) using UBE2S polyclonal antibody ( Cat # PAB1701 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the UBE2S gene ( Lane 2 ) ( Origene Technologies ) .
UBE2S polyclonal antibody ( Cat # PAB1701 ) is used in Western blot to detect UBE2S in Jurkat cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2S polyclonal antibody ( Cat # PAB1702 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Western blot analysis of UBE2S ( arrow ) using UBE2S polyclonal antibody ( Cat # PAB1702 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the UBE2S gene ( Lane 2 ) ( Origene Technologies ) .
UBE2S polyclonal antibody ( Cat # PAB1702 ) is used in Western blot to detect UBE2S in HL-60 cell lysate.
Recombinant human UBE2S, 1-222 aa, His-tagged: 15% SDS-PAGE (3 µg)
Immunofluorescence of purified MaxPab antibody to UBE2S on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of UBE2S expression in transfected 293T cell line (H00027338-T01) by UBE2S MaxPab polyclonal antibody.Lane 1: UBE2S transfected lysate(24.42 KDa).Lane 2: Non-transfected lysate.
Human 293T; WB Suggested Anti-UBE2S Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. UBE2S is supported by BioGPS gene expression data to be expressed in HEK293T.; UBE2S antibody - N-terminal region (ARP43155_P050) in Human 293T cells using Western Blot
Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green).
Immunofluorescence: Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green).
Western blot analysis: Cell lysates of 293T (50 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2S (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2S (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunoprecipitation of UBE2S transfected lysate using anti-UBE2S MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with UBE2S MaxPab mouse polyclonal antibody (B01) (H00027338-B01).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (RC224851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
HEK293T cells transfected with either RC224851 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2S antibody(TA505168), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-UBE2S antibody(TA505168),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-UBE2S antibody(TA505168),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (RC224851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
HEK293T cells transfected with either RC224851 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2S antibody(TA505169), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-UBE2S antibody(TA505169),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-UBE2S antibody(TA505169),(Red), compared to a nonspecific negative control antibody,(Blue).
Anti-UBE2S mouse monoclonal antibody (TA505185) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S(RC224851).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (RC224851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
Anti-UBE2S mouse monoclonal antibody (TA505186) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S(RC224851).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (RC224851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
HEK293T cells transfected with either RC224851 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2S antibody(TA505186), and then analyzed by flow cytometry.
Anti-UBE2S mouse monoclonal antibody (TA505187) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2S(RC224851).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (RC224851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2S monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC224851 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-UBE2S antibody(TA505187), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UBE2S rabbit polyclonal antibody. (TA590247)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-UBE2S rabbit polyclonal antibody. (TA590247)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-UBE2S rabbit polyclonal antibody. (TA590247)
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-UBE2S rabbit polyclonal antibody. (TA590247)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-UBE2S rabbit polyclonal antibody. (TA590247)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2S (RC224851, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2S.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2S polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
The anti-E2EPF Pab (Cat. #TA302236) is used in Western blot to detect E2EPF in Jurkat cell lysate.
Western blot analysis of E2EPF (arrow) using E2EPF Antibody (C-term) (Cat.#TA302236). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the E2EPF gene (Lane 2) (Origene Technologies).
E2EPF Antibody (C-term) (Cat. #TA302236)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of E2EPF Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
The anti-E2EPF Pab (Cat. #TA302237) is used in Western blot to detect E2EPF in HL-60 cell lysate.
Western blot analysis of E2EPF (arrow) using E2EPF Antibody (N-term) (Cat.#TA302237). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
WB Suggested Anti-UBE2S Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysateUBE2S is supported by BioGPS gene expression data to be expressed in HEK293T
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human testis tissue, Primary antibody: (UBE2S Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (UBE2S Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (UBE2S Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (6)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA505168 | UBE2S antibody |
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| UBE2S mouse monoclonal antibody, clone OTI1B5 (formerly 1B5) | |||||||
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Mouse | IgG1 | OTI1B5 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA505169 | UBE2S antibody |
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| UBE2S mouse monoclonal antibody, clone OTI4B8 (formerly 4B8) | |||||||
 |
Mouse | IgG1 | OTI4B8 | Purified | Hu | F, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA505187 | UBE2S antibody |
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| UBE2S mouse monoclonal antibody, clone OTI4H3 (formerly 4H3) | |||||||
 |
Mouse | IgG1 | OTI4H3 | Purified | Hu, Mky, Ms | F, ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA590247 | UBE2S antibody |
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| Rabbit Polyclonal UBE2S Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Mky, Ms | P, WB |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody! |
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| AM09138PU-N | UBE2S antibody |
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 |
Mouse | IgG2a | AT2C12 | Purified | Hu | E, ICC/IF, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AM09138PU-S | UBE2S antibody |
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|
Mouse | IgG2a | AT2C12 | Purified | Hu | E, ICC/IF, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA302236 | UBE2S (C-term) antibody |
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| Rabbit Polyclonal Antibody against UBE2S (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA302237 | UBE2S (N-term) antibody |
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| Rabbit Polyclonal Antibody against UBE2S (N-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA349911 | UBE2S antibody |
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| Rabbit Polyclonal Anti-UBE2S Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA505185 | UBE2S antibody |
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| UBE2S mouse monoclonal antibody, clone OTI4E4 (formerly 4E4) | |||||||
 |
Mouse | IgG1 | OTI4E4 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA505186 | UBE2S antibody |
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| UBE2S mouse monoclonal antibody, clone OTI4G2 (formerly 4G2) | |||||||
 |
Mouse | IgG1 | OTI4G2 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP324851 | UBE2S |
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| Recombinant protein of human ubiquitin-conjugating enzyme E2S (UBE2S) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720259 | UBE2S |
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| Recombinant protein of human ubiquitin-conjugating enzyme E2S (UBE2S) | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402342 | UBE2S overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |