UBE2T antibody
Principal name
UBE2T antibody
Alternative names for UBE2T antibody
Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T, Ubiquitin carrier protein T, HSPC150
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of UBE2T antibody
- Kit Component:
- - KN318591G1, Ube2t gRNA vector 1 in pCas-Guide vector
- KN318591G2, Ube2t gRNA vector 2 in pCas-Guide vector
- KN318591D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Machida YJ, Machida Y, Chen Y, Gurtan AM, Kupfer GM, D'Andrea AD, Dutta A.
UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation.
Mol Cell. 2006 Aug;23(4):589-96.
PMID: 16916645
UBE2T monoclonal antibody (M01), clone 1E12-4A3 Western Blot analysis of UBE2T expression in Hela ( Cat # L013V1 ).
UBE2T polyclonal antibody ( Cat # PAB6306 ) staining ( 0.1 µg/ml ) of K-562 lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of UBE2T expression in transfected 293T cell line (H00029089-T01) by UBE2T MaxPab polyclonal antibody.Lane 1: UBE2T transfected lysate(21.67 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of UBE2T expression in transfected 293T cell line (H00029089-T02) by UBE2T MaxPab polyclonal antibody.Lane 1: UBE2T transfected lysate(22.50 KDa).Lane 2: Non-transfected lysate.
Recombinant human UBE2T, 1-197 aa, His-tagged: 15% SDS-PAGE (3 ug)
Human HepG2; WB Suggested Anti-UBE2T Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; UBE2T antibody - N-terminal region (ARP43145_P050) in Human HepG2 cells using Western Blot
Western blot analysis of UBE2T expression in Jurkat (A); HepG2 (B); HeLa (C) whole cell lysates.
Anti-UBE2T mouse monoclonal antibody (TA501645) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2T(RC200748).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2T (RC200748, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2T.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-UBE2T monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
TA302510 (0.1µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts from HeLa cells, Jurkat cells and K562 cells, using UBE2T antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-UBE2T Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302510 | UBE2T (C-term) antibody |
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| Goat Polyclonal Antibody against HSPC150 / UBE2T | |||||||
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Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA312363 | UBE2T antibody |
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| Rabbit polyclonal anti-UBE2T antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA501645 | UBE2T antibody |
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| anti-UBE2T mouse monoclonal antibody, clone OTI2F5 (formerly 2F5) | |||||||
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Mouse | IgG2a | OTI2F5 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300748 | UBE2T |
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| Recombinant protein of human ubiquitin-conjugating enzyme E2T (putative) (UBE2T) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720164 | UBE2T |
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| Recombinant protein of human ubiquitin-conjugating enzyme E2T (putative) (UBE2T) | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||