UBTD1 antibody
Principal name
UBTD1 antibody
Alternative names for UBTD1 antibody
Ubiquitin domain-containing protein 1
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of UBTD1 antibody
- Kit Component:
- - KN203403G1, UBTD1 gRNA vector 1 in pCas-Guide vector
- KN203403G2, UBTD1 gRNA vector 2 in pCas-Guide vector
- KN203403D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with UBTD1 polyclonal antibody ( Cat # PAB2253 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of UBTD1 ( arrow ) using rabbit UBTD1 polyclonal antibody ( Cat # PAB2253 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the UBTD1 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of UBTD1 polyclonal antibody ( Cat # PAB2253 ) in Hela cell line lysates ( 35 µg/lane ) . UBTD1 ( arrow ) was detected using the purified polyclonal antibody.
Western Blot analysis of UBTD1 expression in transfected 293T cell line (H00080019-T01) by UBTD1 MaxPab polyclonal antibody.Lane 1: UBTD1 transfected lysate(24.97 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-UBTD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; UBTD1 antibody - middle region (ARP59397_P050) in Human Jurkat cells using Western Blot
Western blot analysis of UBTD1 (arrow) using rabbit polyclonal UBTD1 Antibody (AP54448PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBTD1 gene.
Immunohistochemical analysis of UBTD1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of UBTD1 expression in HepG2 (A); K562 (B) whole cell lysates.
Western blot analysis of UBTD1(arrow) using rabbit polyclonal UBTD1 Antibody (C-term G195) polyclonal antibody(Cat.#TA302267). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBTD1 gene (Lane 2) (Origene Technologies).
Western blot analysis of UBTD1 Antibody (C-term G195) (Cat.#TA302267) in Hl60 cell line lysates (35ug/lane). UBTD1(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with UBTD1 Antibody (C-term G195)(Cat.#TA302267), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using UBTD1 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-UBTD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302267 | UBTD1 (C-term) antibody |
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| Rabbit Polyclonal Antibody against UBTD1 (C-term G195) | |||||||
 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA326217 | UBTD1 antibody |
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| Rabbit polyclonal UBTD1 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP54448PU-N | UBTD1 antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303403 | UBTD1 |
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| Recombinant protein of human ubiquitin domain containing 1 (UBTD1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410953 | UBTD1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209799L2V | Lenti ORF particles, Ubtd1 (GFP-tagged ORF) - Rat ubiquitin domain containing 1 (Ubtd1), 200 uL, >10^7 TU/mL |
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|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR209799L1V | Lenti ORF particles, Ubtd1 (Myc-DDK-tagged ORF) - Rat ubiquitin domain containing 1 (Ubtd1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||