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UCHL5 antibody

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Principal name

UCHL5 antibody

Alternative names for UCHL5 antibody

UCH-L5, Ubiquitin thioesterase L5, UCH37, AD-019, CGI-70, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin C-terminal hydrolase UCH37

SwissProt ID

Q06AT3 (Pig), Q9WUP7 (Mouse), Q9XSJ0 (Bovin), Q9Y5K5 (Human)

Gene ID

51377 (UCHL5), 56207 (Uchl5)

Ncbi ID

9606, NP_057068, NP_001186190.1, NP_001186190, NP_001153338.1, NM_001159866, NM_019562, NM_015984

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of UCHL5 antibody

Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.

General readings

Yao T., et al. (2006) Nat. Cell Biol. 8:994-1002
Nishio K, et al.(2009) Biochem Biophys Res Commun. 390(3):855-60.

PAB3907 - UCHL5
HE staining of frozen human ovarian cancer tissue reacted with UCHL5 polyclonal antibody ( Cat # PAB3907 ) at a 1 : 250 dilution. Levels using the antibody on frozen tissue array ( A ) correlated well with the mRNA expression levels detected by Agilent expression microarray ( B ) . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles.
PAB3907 - UCHL5
The UCHL5 polyclonal antibody ( Cat # PAB3907 ) is used in Western blot to detect UCHL5 in mouse kidney tissue lysate.
AP11996PU-N - UCHL5
HE staining of frozen human ovarian cancer tissue reacted with the primary antibody at a 1:250 dilution. Levels using the antibody on frozen tissue array (A )correlated well with the mRNA expression levels detected by Agilent expression microarray (B). This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles.
AP11996PU-N - UCHL5
Western Blot analysis using anti-UCHL5 (N-term) Pab (Cat.#AP11996PU-N) to detect UCHL5 in mouse kidney tissue lysate.
H00051377-B02P - UCHL5
UCHL5 MaxPab polyclonal antibody. Western Blot analysis of UCHL5 expression in human placenta.
H00051377-B02P - UCHL5
Western Blot analysis of UCHL5 expression in transfected 293T cell line (H00051377-T01) by UCHL5 MaxPab polyclonal antibody.Lane 1: UCHL5 transfected lysate(35.86 KDa).Lane 2: Non-transfected lysate.
ARP56797_P050 - UCHL5
Zebrafish skin; WB Suggested Anti-UCHL5 Antibody . . Positive Control: Lane 1: 30ug Zebrafish skin lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Anti-rabbit-HRP . Secondry Antibody Dilution : 1:4000. Submitted by: William Tse; UCHL5 antibody - middle region (ARP56797_P050) in Zebrafish skin cells using Western Blot
ARP56797_P050 - UCHL5
Human Small Intestine; WB Suggested Anti-UCHL5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Small Intestine; UCHL5 antibody - middle region (ARP56797_P050) in Human Small Intestine cells using Western Blot
ARP59295_P050 - UCHL5
Hum. Fetal Muscle; Host:Rabbit. Target Name:UCHL5. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; UCHL5 antibody - C-terminal region (ARP59295_P050) in Hum. Fetal Muscle cells using Western Blot
ARP59295_P050 - UCHL5
Human HepG2; WB Suggested Anti-UCHL5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; UCHL5 antibody - C-terminal region (ARP59295_P050) in Human HepG2 cells using Western Blot
ARP59296_P050 - UCHL5
Human HepG2; WB Suggested Anti-UCHL5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; UCHL5 antibody - middle region (ARP59296_P050) in Human HepG2 cells using Western Blot
AR39009PU-L - UCHL5
AR39009PU-L - UCHL5
Recombinant human UCHL5, 1-329aa, His-tagged
TP302126 - UCHL5
TA324998 - UCHL5
UCH37(UCHL5) Antibody(Cat. #TA324998) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the UCH37 (UCHL5) antibody detected the UCH37 (UCHL5) protein (arrow).
TA325168 - UCHL5
UCH37 (UCHL5) Antibody (N-term) (Cat. #TA325168) western blot analysis in 293,Hela,293T cell line lysates (35ug/lane).This demonstrates the UCHL5 antibody detected the UCHL5 protein (arrow).
TA342561 - UCHL5
WB Suggested Anti-UCHL5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
TA342561 - UCHL5
Host:Rabbit; Target Name:UCHL5; Sample Tissue:Human Fetal Muscle; Antibody Dilution: 1.0 ug/ml
TA342562 - UCHL5
WB Suggested Anti-UCHL5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
TA345084 - UCHL5
WB Suggested Anti-UCHL5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Small Intestine
TA345084 - UCHL5
WB Suggested Anti-UCHL5 Antibody ; Positive Control: Lane 1: 30ug Zebrafish skin lysate; Primary Antibody Dilution : 1:1000; Secondary Antibody : Anti-rabbit-HRP; Secondry Antibody Dilution : 1:4000; Submitted by: William Tse

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

UCHL5 antibody
  Mouse monoclonal UCH37(UCHL5) Antibody(Ascites)  
UCH37(UCHL5) Antibody(Cat. #TA324998) western blot analysis in 293,Hela cell line lysates (35ug/lane).This demonstrates the UCH37 (UCHL5) antibody detected the UCH37 (UCHL5) protein (arrow). Mouse IgG1 854CT5.5.6 Ascites Hu WB
0.1 ml / €415.00
  OriGene Technologies, Inc.

UCHL5 antibody
  Mouse monoclonal UCH37 (UCHL5) Antibody (N-term)  
UCH37 (UCHL5) Antibody (N-term) (Cat. #TA325168) western blot analysis in 293,Hela,293T cell line lysates (35ug/lane).This demonstrates the UCHL5 antibody detected the UCHL5 protein (arrow). Mouse IgG1 854CT5.5.6 Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies, Inc.

UCHL5 (N-term) antibody
HE staining of frozen human ovarian cancer tissue reacted with the primary antibody at a 1:250 dilution. Levels using the antibody on frozen tissue array (A )correlated well with the mRNA expression levels detected by Agilent expression microarray (B). This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

UCHL5
  Purified recombinant protein of Homo sapiens ubiquitin carboxyl-terminal hydrolase L5 (UCHL5)  
UCHL5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Uchl5 (GFP-tagged) - Mouse ubiquitin carboxyl-terminal esterase L5 (Uchl5), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Uchl5 (GFP-tagged) - Mouse ubiquitin carboxyl-terminal esterase L5 (Uchl5), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Uchl5 (GFP-tagged) - Mouse ubiquitin carboxyl-terminal esterase L5 (Uchl5), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, Uchl5 (GFP-tagged) - Mouse ubiquitin carboxyl-terminal esterase L5 (Uchl5), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,240.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Uchl5 (Myc-DDK-tagged) - Mouse ubiquitin carboxyl-terminal esterase L5 (Uchl5), transcript variant 1, 200 uL, >10^7 TU/mL
Lenti ORF particles, Uchl5 (Myc-DDK-tagged) - Mouse ubiquitin carboxyl-terminal esterase L5 (Uchl5), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Uchl5 (Myc-DDK-tagged) - Mouse ubiquitin carboxyl-terminal esterase L5 (Uchl5), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, Uchl5 (Myc-DDK-tagged) - Mouse ubiquitin carboxyl-terminal esterase L5 (Uchl5), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.
 

8 Item(s)

per page

Available Filters

Product Type
  1. Mouse Lenti ORF Particle (4)
  2. Primary Antibody (3)
  3. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (2)
  2. Polyclonal (1)
Target
  1. UCHL5 (4)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Paraffin Sections (P) (1)
Presentation
  1. Purified (2)
  2. Ascites (1)
Host
  1. Mouse (2)
  2. Rabbit (1)
Images
  1. 1 image (4)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Frozen sections (C) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (8)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
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