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UGP2 antibody

Principal name

UGP2 antibody

Alternative names for UGP2 antibody

UGP1, UDP-glucose pyrophosphorylase, UGPase, UDPGP, UTP-glucose-1-phosphate uridylyltransferase

SwissProt ID

P79303 (Pig), Q07130 (Bovin), Q16851 (Human)

Gene ID

7360 (UGP2), 281565

Ncbi ID

9606, NP_001001521, NP_776637, NP_001001521.1, NP_006750, NM_001001521

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Dot blot (Dot), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R)

Background of UGP2 antibody

UGP2 is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. The eukaryotic enzyme has no significant sequence similarity to the prokaryotic enzyme.The enzyme encoded by this gene is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. The eukaryotic enzyme has no significant sequence similarity to the prokaryotic enzyme. Two transcript variants encoding different isoforms have been found for this gene.

General readings

Fuhring,J., et al. (2013) Glycobiology 23 (4), 426-437

Western Blot analysis of UGP2 expression in transfected 293T cell line by UGP2 monoclonal antibody (M01), clone 3H3.Lane 1: UGP2 transfected lysate(56.9 KDa).Lane 2: Non-transfected lysate.

H00007360-M01

Western blot analysis of UGP2 over-expressed 293 cell line, cotransfected with UGP2 Validated Chimera RNAi ( Cat # H00007360-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with UGP2 monoclonal antibody (M01), clone 3H3 (Cat # H00007360-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

H00007360-M01

UGP2 monoclonal antibody (M01), clone 3H3 Western Blot analysis of UGP2 expression in HeLa ( Cat # L013V1 ).

H00007360-M01

UGP2 monoclonal antibody (M01), clone 3H3. Western Blot analysis of UGP2 expression in NIH/3T3 ( Cat # L018V1 ).

H00007360-M01

UGP2 monoclonal antibody (M01), clone 3H3. Western Blot analysis of UGP2 expression in Raw 264.7 ( Cat # L024V1 ).

H00007360-M01

UGP2 monoclonal antibody (M01), clone 3H3. Western Blot analysis of UGP2 expression in PC-12 ( Cat # L012V1 ).

H00007360-M01

Detection limit for recombinant GST tagged UGP2 is approximately 0.1ng/ml as a capture antibody.

H00007360-M01

UGP2 polyclonal antibody (A01), Lot # 060707JCS1 Western Blot analysis of UGP2 expression in HepG2 ( Cat # L019V1 ).

H00007360-A01

Western Blot analysis of UGP2 expression in transfected 293T cell line (H00007360-T01) by UGP2 MaxPab polyclonal antibody.Lane 1: UGP2 transfected lysate(56.90 KDa).Lane 2: Non-transfected lysate.

H00007360-B01

Human HepG2; WB Suggested Anti-UGP2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate. UGP2 is supported by BioGPS gene expression data to be expressed in HepG2.; UGP2 antibody - N-terminal region (ARP48303_P050) in Human HepG2 cells using Western Blot

ARP48303_P050

TP300246

TP308376

WB Suggested Anti-UGP2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate.UGP2 is supported by BioGPS gene expression data to be expressed in HepG2

TA346610

CRISPR

Catalog No.	Species	Donor Vector
KN200246	UGP2 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN200246BN	UGP2 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN200246LP	UGP2 - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN200246RB	UGP2 - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP300246	UGP2 (transcript variant 2)
	Recombinant protein of human UDP-glucose pyrophosphorylase 2 (UGP2), transcript variant 2
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

TP308376	UGP2 (transcript variant 1)
	Recombinant protein of human UDP-glucose pyrophosphorylase 2 (UGP2), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY424368	UGP2 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

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