UHRF2 antibody
Principal name
UHRF2 antibody
Alternative names for UHRF2 antibody
NIRF, RNF107, E3 ubiquitin-protein ligase UHRF2, Ubiquitin-like PHD and RING finger domain-containing protein 2, Ubiquitin-like-containing PHD and RING finger domains protein 2, Np95/ICBP90-like RING finger protein, Nuclear zinc finger protein Np97, RING finger protein 107
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection))
Background of UHRF2 antibody
- Kit Component:
- - KN219579G1, NIRF gRNA vector 1 in pCas-Guide vector
- KN219579G2, NIRF gRNA vector 2 in pCas-Guide vector
- KN219579D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Li,Y., et al., (2004) Biochem. Biophys. Res. Commun. 319 (2), 464-468
Human Heart; UHRF2 antibody - N-terminal region (ARP34772_P050) in Human Heart cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-UHRF2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; UHRF2 antibody - N-terminal region (ARP34772_P050) in Human Jurkat cells using Western Blot
Western blot analysis of UHRF2 over-expressed 293 cell line, cotransfected with UHRF2 Validated Chimera RNAi ( Cat # H00115426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with UHRF2 monoclonal antibody (M01), clone 3A11 (Cat # H00115426-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of UHRF2 expression in transfected 293T cell line by UHRF2 monoclonal antibody (M01), clone 3A11.Lane 1: UHRF2 transfected lysate(56.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of UHRF2 expression in transfected 293T cell line (H00115426-T01) by UHRF2 MaxPab polyclonal antibody.Lane 1: UHRF2 transfected lysate(55.33 KDa).Lane 2: Non-transfected lysate.
Human Liver; WB Suggested Anti-UHRF2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Liver; UHRF2 antibody - middle region (ARP34620_P050) in Human Liver cells using Western Blot
Mouse Pancreas; WB Suggested Anti-Uhrf2 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Pancreas; Uhrf2 antibody - N-terminal region (ARP34771_P050) in Mouse Pancreas cells using Western Blot
HCT116; WB Suggested Anti-Uhrf2 Antibody . . Positive Control: Lane 1: 60ug HCT116 lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Anti rabbit-HRP . Secondry Antibody Dilution : 1:5,000. Submitted by: Chinweike Ukomadu, Brigham and Women's Hospital, Boston; Uhrf2 antibody - N-terminal region (ARP34771_P050) in HCT116 cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UHRF2 (RC219579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UHRF2.
ELISA: NIRF Antibody
Western blot analysis of extracts of various cell lines, using UHRF2 antibody.
WB Suggested Anti-UHRF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver
WB Suggested Anti-UHRF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate
Human Heart
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA327339 | UHRF2 antibody |
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| UHRF2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA351439 | UHRF2 antibody |
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| Rabbit Polyclonal Anti-UHRF2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA590391 | UHRF2 antibody |
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| Rabbit Polyclonal NIRF Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
50 µg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP319579 | UHRF2 |
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| Recombinant protein of human ubiquitin-like with PHD and ring finger domains 2 (UHRF2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY407258 | UHRF2 overexpression lysate |
|
|
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR210744L2V | Lenti ORF particles, Uhrf2 (GFP-tagged) - Mouse ubiquitin-like, containing PHD and RING finger domains 2 (Uhrf2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,600.00
|
|
| OriGene Technologies, Inc. | ||
| MR210744L1V | Lenti ORF particles, Uhrf2 (Myc-DDK-tagged) - Mouse ubiquitin-like, containing PHD and RING finger domains 2 (Uhrf2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,440.00
|
|
| OriGene Technologies, Inc. | ||