USO1 antibody
Principal name
USO1 antibody
Alternative names for USO1 antibody
USO-1, VDP, General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking protein
Ncbi ID
9606, NP_003706.1, NP_003706, NM_019379, NM_003715, NP_062363.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of USO1 antibody
- Kit Component:
- - KN318763G1, Uso1 gRNA vector 1 in pCas-Guide vector
- KN318763G2, Uso1 gRNA vector 2 in pCas-Guide vector
- KN318763D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Malsam J, Satoh A, Pelletier L, Warren G. Golgin tethers define subpopulations of COPI vesicles. Science. 2005 Feb 18;307(5712):1095-8.
USO1 polyclonal antibody ( Cat # PAB6897 ) ( 1 µg/ml ) staining of human kidney lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Immunoperoxidase of monoclonal antibody to USO1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
USO1 monoclonal antibody (M03), clone 3F4. Western Blot analysis of USO1 expression in HeLa ( Cat # L013V1 ).
TA303068 (1µg/ml) staining of human kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of fetal kidney lysates with anti-VDP antibody (1:250). This antibody identified a band of ~110kDa in human fetal kidney lysates.
Predicted band size: 108 kDa . Positive control: Human fetal kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/400 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds)
Predicted cell location: Cytoplasm. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using USO1 antibody at dilution 1/13, on the right is treated with the synthetic peptide. (Original magnification:x200)
WB Suggested Anti-USO1 Antibody; Titration: 1.0 ug/ml; Positive Control: 293T Whole Cell USO1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA303068 | USO1 antibody |
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| Goat Polyclonal Antibody against VDP | |||||||
 |
Goat | Aff - Purified | Hu | WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA310083 | USO1 antibody |
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| Rabbit anti-VDP polyclonal antibody | |||||||
 |
Rabbit | Purified | Hu | WB |
0.1 ml /
€220.00
|
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| OriGene Technologies, Inc. | |||||||
| TA322828 | USO1 antibody |
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| Anti-USO1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307614 | USO1 |
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| Recombinant protein of human USO1 homolog, vesicle docking protein (yeast) (USO1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR212110L2V | Lenti ORF particles, Uso1 (GFP-tagged ORF) - Rat USO1 homolog, vesicle docking protein (yeast) (Uso1), 200 uL, >10^7 TU/mL |
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 |
200 µl /
€1,830.00
|
|
| OriGene Technologies, Inc. | ||
| RR212110L1V | Lenti ORF particles, Uso1 (Myc-DDK-tagged ORF) - Rat USO1 homolog, vesicle docking protein (yeast) (Uso1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,650.00
|
|
| OriGene Technologies, Inc. | ||