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USP12 antibody

Principal name

USP12 antibody

Alternative names for USP12 antibody

Ubiquitin carboxyl-terminal hydrolase 12, UBH1, USP12L1, Ubiquitin thioesterase 12, Ubiquitin-specific-processing protease 12, Deubiquitinating enzyme 12, Ubiquitin-hydrolyzing enzyme 1

SwissProt ID

O75317 (Human), Q9D9M2 (Mouse)

Gene ID

219333 (USP12), 22217 (Usp12)

Ncbi ID

9606, 5874, NP_872294, NP_872294.2, NM_182488, NP_035799.1, NM_011669

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of USP12 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

USP12 polyclonal antibody (A01), Lot # 051115JC01 Western Blot analysis of USP12 expression in MCF-7 ( Cat # L046V1 ).

H00219333-A01

Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP12 polyclonal antibody ( Cat # PAB4195 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .

PAB4195

The USP12 polyclonal antibody ( Cat # PAB4195 ) is used in Western blot to detect USP12 in mouse kidney tissue lysate .

PAB4195

The USP12 polyclonal antibody ( Cat # PAB4196 ) is used in Western blot to detect USP12 in A-375 cell lysate .

PAB4196

Western Blot analysis of USP12 expression in transfected 293T cell line (H00219333-T01) by USP12 MaxPab polyclonal antibody.Lane 1: USP12 transfected lysate(40.7 KDa).Lane 2: Non-transfected lysate.

H00219333-B01P

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AP12025PU-N

Western blot analysis of anti-USP12 Pab (Cat.#AP12025PU-N) to detect USP12 in mouse kidney tissue lysate.

AP12025PU-N

Western blot analysis of anti-USP12 Pab (Cat.#AP12026PU-N) to detect USP12 in A375 cell lysate.

AP12026PU-N

Human Placenta; WB Suggested Anti-USP12 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; USP12 antibody - middle region (ARP55812_P050) in Human Placenta cells using Western Blot

ARP55812_P050

USP12 Antibody (Center) (AP54486PU-N) western blot analysis in mouse bladder tissue lysates (35 µg/lane). This demonstrates the USP12 antibody detected the USP12 protein (arrow).

AP54486PU-N

USP12 Antibody (Center) (AP54486PU-N) western blot analysis in A375 cell line lysates (35 µg/lane). This demonstrates the USP12 antibody detected the USP12 protein (arrow).

AP54486PU-N

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP12 (RC205355, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP12.

TA800028

TA800037

WB Suggested Anti-USP12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Placenta

TA333400

Host: Rabbit; Target Name: Usp12; Sample Tissue: Mouse Stomach lysates; Antibody Dilution: 1.0 ug/ml

TA333885

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA800028	USP12 antibody
	USP12 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)
	Mouse	IgG1	OTI1E3	Purified	Hu	WB	0.1 ml / €360.00
							OriGene Technologies, Inc.

TA800037	USP12 antibody
	USP12 mouse monoclonal antibody, clone OTI2A6 (formerly 2A6)
	Mouse	IgG1	OTI2A6	Purified	Hu	WB	0.1 ml / €379.00
							OriGene Technologies, Inc.

AP12025PU-N	USP12 (N-term) antibody
	Rabbit	Ig		Purified	Hu, Ms	P, WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+1 additional image
AP12026PU-N	USP12 (C-term) antibody
	Rabbit	Ig		Purified	Hu	WB	0.4 ml / €415.00
							OriGene Technologies GmbH
AP54486PU-N	USP12 (Center) antibody
	Rabbit	Ig		Aff - Purified	Hu, Ms	WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.
LY403634	USP12 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR205699L2V	Lenti ORF particles, Usp12 (GFP-tagged) - Mouse ubiquitin specific peptidase 12 (Usp12), 200 uL, >10^7 TU/mL
		200 µl / €930.00
		OriGene Technologies, Inc.

MR205699L1V	Lenti ORF particles, Usp12 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 12 (Usp12), 200 uL, >10^7 TU/mL
		200 µl / €830.00
		OriGene Technologies, Inc.

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