USP13 antibody
Principal name
USP13 antibody
Alternative names for USP13 antibody
Ubiquitin carboxyl-terminal hydrolase 13, ISOT3, ISOT-3, Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, Deubiquitinating enzyme 13, Isopeptidase T-3
SwissProt ID
Q92995 (Human)
Gene ID
8975 (USP13)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of USP13 antibody
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
The USP13 polyclonal antibody ( Cat # PAB4197 ) is used in Western blot to detect USP13 in A-375 cell lysate .
Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP13 polyclonal antibody ( Cat # PAB4198 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
The USP13 polyclonal antibody ( Cat # PAB4198 ) is used in Western blot to detect USP13 in mouse brain tissue lysate .
The USP13 polyclonal antibody ( Cat # PAB4198 ) is used in Western blot to detect USP13 in HeLa cell lysate .
Western Blot analysis of USP13 expression in transfected 293T cell line (H00008975-T01) by USP13 MaxPab polyclonal antibody.Lane 1: USP13 transfected lysate(94.93 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis using anti-USP13 Pab (Cat.#AP12027PU-N) to detect USP13 in A375 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western Blot analysis using anti-USP13 Pab (Cat.#AP12028PU-N) to detect USP13 in HeLa cell lysate.
Human Placenta; WB Suggested Anti-USP13 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; USP13 antibody - N-terminal region (ARP59306_P050) in Human Placenta cells using Western Blot
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-USP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500318, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP13 (RC202190, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP13.
Immunoprecipitation(IP) of USP13 by using TrueMab monoclonal anti-USP13 antibodies (Negative control: IP without adding anti-USP13 antibody.). For each experiment, 500ul of DDK tagged USP13 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP13 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500327, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-USP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500327, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500327, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500327, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500327, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500327, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP13 (RC202190, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP13.
Immunoprecipitation(IP) of USP13 by using TrueMab monoclonal anti-USP13 antibodies (Negative control: IP without adding anti-USP13 antibody.). For each experiment, 500ul of DDK tagged USP13 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug
Immunoprecipitation(IP) of USP13 by using TrueMab monoclonal anti-USP13 antibodies (Negative control: IP without adding anti-USP13 antibody.). For each experiment, 500ul of DDK tagged USP13 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP13 (RC202190, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP13.
Western blot analysis of extracts from mouse brain cells, using USP13 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using USP13 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-USP13 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Placenta
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500318 | USP13 antibody |
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| anti-USP13 mouse monoclonal antibody, clone OTI4G11 (formerly 4G11) | |||||||
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Mouse | IgG1 | OTI4G11 | Purified | Can, Hu, Mky | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +12 additional images |
Highly validated antibody!
|
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| TA500327 | USP13 antibody |
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| anti-USP13 mouse monoclonal antibody, clone OTI4G1 (formerly 4G1) | |||||||
 |
Mouse | IgG1 | OTI4G1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +7 additional images |
Highly validated antibody!
|
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| TA312123 | USP13 antibody |
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| Rabbit polyclonal anti-USP13 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA500367 | USP13 antibody |
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| anti-USP13 mouse monoclonal antibody, clone OTI5B9 (formerly 5B9) | |||||||
 |
Mouse | IgG1 | OTI5B9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP12027PU-N | USP13 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP12028PU-N | USP13 (Center) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN202190 | USP13 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN202190BN | USP13 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN202190LP | USP13 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN202190RB | USP13 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302190 | USP13 |
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| Recombinant protein of human ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||