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USP16 antibody

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Principal name

USP16 antibody

Alternative names for USP16 antibody

Ubiquitin carboxyl-terminal hydrolase 16, MSTP039, Ubiquitin thioesterase 16, Ubiquitin-specific-processing protease 16, Deubiquitinating enzyme 16, Ubiquitin-processing protease UBP-M

SwissProt ID

Q08DA3 (Bovin), Q2KJ09 (Rat), Q99LG0 (Mouse), Q9Y5T5 (Human)

Gene ID

10600 (USP16), 74112 (Usp16), 288306

Ncbi ID

9606, AAR13293, 5874, NP_001001992, NP_001001992.1, NM_006447, NM_001032410, NM_001001992, NP_006438, NP_077220.2, NM_001100501

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of USP16 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

PAB4203 - USP16
Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP16 polyclonal antibody ( Cat # PAB4203 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
PAB4203 - USP16
Western blot analysis of USP16 polyclonal antibody ( Cat # PAB4203 ) in mouse liver tissue lysate ( 35 µg/lane ) . USP16 ( arrow ) was detected using the purified USP16 polyclonal antibody ( Cat # PAB4203 ) ( 1 : 60 dilution )
PAB4204 - USP16
Western blot analysis of USP16 polyclonal antibody ( Cat # PAB4204 ) in HL-60 cell line lysate ( 35 µg/lane ) . USP16 ( arrow ) was detected using the purified USP16 polyclonal antibody ( Cat # PAB4204 ) .
ARP45772_T100 - USP16
Human brain stems (NT2); . Sample Type :. Human brain stem cells (NT2) . Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. Red: USP16 Blue: DAPI. Gene Name :. USP16. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; USP16 antibody - N-terminal region (ARP45772_T100) in Human brain stems (NT2) cells using Immunohistochemistry
ARP45772_T100 - USP16
Human HepG2; WB Suggested Anti-USP16 Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; USP16 antibody - N-terminal region (ARP45772_T100) in Human HepG2 cells using Western Blot
AP12033PU-N - USP16
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP12033PU-N - USP16
Western blot analysis of anti-USP16 Pab (Cat.#AP12033PU-N) in mouse liver tissue lysate (35ug/lane). USP16 (arrow) was detected using the purified Pab (1:60 dilution).
AP12034PU-N - USP16
Western blot analysis of anti-USP16 Pab (Cat.#AP12034PU-N) in HL60 cell line lysate (35ug/lane). USP16 (arrow) was detected using the purified Pab.
H00010600-B01P - USP16
Immunofluorescence of purified MaxPab antibody to USP16 on HeLa cell. [antibody concentration 10 ug/ml]
H00010600-B01P - USP16
Western Blot analysis of USP16 expression in transfected 293T cell line (H00010600-T01) by USP16 MaxPab polyclonal antibody.Lane 1: USP16 transfected lysate(90.42 KDa).Lane 2: Non-transfected lysate.
AP54487PU-N - USP16
USP16 Antibody (N-term) (AP54487PU-N) western blot analysis in SK-BR-3 cell line lysates (35 µg/lane). This demonstrates the USP16 antibody detected the USP16 protein (arrow).
AP33410PU-N - USP16
AP33410PU-N (4µg/ml) staining of paraffin embedded Human Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
TA505771 - USP16
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP16 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
TA505771 - USP16
E.coli lysate (5 ug, left lane) and E.coli lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 137-466 of human USP16(NP_001001992) were separated by SDS-PAGE and immunoblotted with anti-USP16.
TP322609 - USP16
TA332969 - USP16
Western blot analysis of extracts of various cell lines, using USP16 antibody.
TA338905 - USP16
WB Suggested Anti-USP16 Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysate
TA338905 - USP16
Sample Type: Human brain stem cells (NT2) Primary Antibody Dilution: 1: 500; Secondary Antibody: Goat anti-rabbit Alexa Fluor 594; Secondary Antibody: ilution: 1: 1000; Color/Signal Descriptions: Red: USP16 Blue: DAPI; Gene Name: USP16; Submitted by: Dr.

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

USP16 antibody
  Rabbit anti-USP16 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using USP16 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.

USP16 antibody
  USP16 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)  
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP16 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG2a OTI1B4 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

USP16 (N-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

USP16 (Center) antibody
Western blot analysis of anti-USP16 Pab (Cat.#AP12034PU-N) in HL60 cell line lysate (35ug/lane). USP16 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

USP16 antibody
AP33410PU-N (4µg/ml) staining of paraffin embedded Human Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P
0.1 mg / €380.00
  OriGene Technologies GmbH

USP16 (N-term) antibody
USP16 Antibody (N-term) (AP54487PU-N) western blot analysis in SK-BR-3 cell line lysates (35 µg/lane). This demonstrates the USP16 antibody detected the USP16 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

USP16 (transcript variant 1)
  Recombinant protein of human ubiquitin specific peptidase 16 (USP16), transcript variant 1  
USP16 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

USP16 overexpression lysate
USP16 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

USP16 overexpression lysate
USP16 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

USP16 overexpression lysate
USP16 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

USP16 overexpression lysate
USP16 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

USP16 overexpression lysate
USP16 overexpression lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Usp16 (GFP-tagged ORF) - Rat ubiquitin specific peptidase 16 (Usp16), 200 uL, >10^7 TU/mL
Lenti ORF particles, Usp16 (GFP-tagged ORF) - Rat ubiquitin specific peptidase 16 (Usp16), 200 uL, >10^7 TU/mL  
200 µl / €1,640.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Usp16 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 16 (Usp16), 200 uL, >10^7 TU/mL
Lenti ORF particles, Usp16 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 16 (Usp16), 200 uL, >10^7 TU/mL  
200 µl / €1,480.00
  OriGene Technologies, Inc.
 

14 Item(s)

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14 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Lysate (5)
  2. Primary Antibody (6)
  3. Protein | Growth Factor (1)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (5)
  2. Monoclonal (1)
Target
  1. USP16 (7)
Property
  1. Center (1)
  2. N-term (2)
Reactivity
  1. Human (Hu) (6)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
  4. Bovine (Bov) (1)
  5. Canine (Can) (1)
  6. Porcine (Por) (1)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Paraffin Sections (P) (2)
  3. Enzyme Immunoassay (E) (1)
Presentation
  1. Purified (4)
  2. Aff - Purified (2)
Host
  1. Rabbit (4)
  2. Goat (1)
  3. Mouse (1)
Images
  1. 1 image (7)
Images: Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (14)
Service
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