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USP18 antibody

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Principal name

USP18 antibody

Alternative names for USP18 antibody

Ubl carboxyl-terminal hydrolase 18, ISG43, Ubl thioesterase 18, ISG15-specific-processing protease, 43 kDa ISG15-specific protease

SwissProt ID

Q9UMW8 (Human), Q9WTV6 (Mouse)

Gene ID

11274 (USP18)

Ncbi ID

NP_059110.2, 9606, NP_059110, NM_017414

Available reactivities

Hu (Human), Chimp (Chimpanzee), Ms (Mouse), Por (Porcine), Orang-Utan, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Immunoprecipitation (IP)

Background of USP18 antibody

USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.[supplied by OMIM]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-232 AC008079.24 80964-81195 233-495 AC008079.24 88531-88793 496-592 AC008079.24 91145-91241 593-738 AC008079.24 92763-92908 739-818 AC008079.24 98228-98307 819-965 AC008079.24 98863-99009 966-1061 AC008079.24 100817-100912 1062-1229 AC008079.24 101726-101893 1230-1361 AC008079.24 104123-104254 1362-1411 AC008079.24 104766-104815 1412-2037 AC008079.24 107745-108370

General readings

Yan,M., (2007) Blood 110 (1), 305-312

H00011274-B01 - USP18
Western Blot analysis of USP18 expression in transfected 293T cell line (H00011274-T01) by USP18 MaxPab polyclonal antibody.Lane 1: USP18 transfected lysate(41.03 KDa).Lane 2: Non-transfected lysate.
H00011274-B02 - USP18
Immunofluorescence of purified MaxPab antibody to USP18 on HeLa cell. [antibody concentration 10 ug/ml]
H00011274-B02 - USP18
Western Blot analysis of USP18 expression in transfected 293T cell line (H00011274-T02) by USP18 MaxPab polyclonal antibody.Lane 1: USP18 transfected lysate(40.92 KDa).Lane 2: Non-transfected lysate.
AP07181PU-N - USP18
Heart: Formalin-Fixed Paraffin-Embedded (FFPE)
H00011274-B03P - USP18
Western Blot analysis of USP18 expression in transfected 293T cell line (H00011274-T04) by USP18 MaxPab polyclonal antibody.Lane 1: USP18 transfected lysate(40.92 KDa).Lane 2: Non-transfected lysate.
H00011274-B02P - USP18
Immunofluorescence of purified MaxPab antibody to USP18 on HeLa cell. [antibody concentration 10 ug/ml]
H00011274-B02P - USP18
Western Blot analysis of USP18 expression in transfected 293T cell line (H00011274-T02) by USP18 MaxPab polyclonal antibody.Lane 1: USP18 transfected lysate(40.92 KDa).Lane 2: Non-transfected lysate.
PAB10241 - USP18
Western blot using USP18 polyclonal antibody ( Cat # PAB10241 ) shows detection of a band ~43kDa band ( arrowhead ) believed to be to USP18 in lysates from HeLa nuclear extracts ( lane 1 ) and whole cell lysates from HeLa ( lane 2 ), A-431 ( lane 3 ), Jurkat ( lane 4 ) and HEK293 ( lane 5 ).Approximately 18 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1 : 500 dilution of USP18 polyclonal antibody.Signal was detected using standard techniques.The strong band between the 37 kDa and 50 kDa markers is expected to be USP18.The other bands are probably non-specific.
ARP46253_P050 - USP18
Mouse Brain; USP18 antibody - N-terminal region (ARP46253_P050) validated by WB using Mouse Brain lysate at 2ug/ml.; USP18 antibody - N-terminal region (ARP46253_P050) in Mouse Brain cells using Western Blot
ARP46253_P050 - USP18
Human HeLa; WB Suggested Anti-USP18 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; USP18 antibody - N-terminal region (ARP46253_P050) in Human HeLa cells using Western Blot
TA319486 - USP18
WB using Anti-UBP43 antibody shows detection of a band ~43 kDa band (arrowhead) in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody.The strong band between the 37kD and 50kD markers is expected to be UBP43.
TA339338 - USP18
USP18 antibody - N-terminal region validated by WB using Mouse Brain lysate at 2 ug/ml.
TA339338 - USP18
WB Suggested Anti-USP18 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate

7 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

USP18 (40-49) antibody
  Rabbit polyclonal anti-UBP43 antibody  
WB using Anti-UBP43 antibody shows detection of a band ~43 kDa band (arrowhead) in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody.The strong band between the 37kD and 50kD markers is expected to be UBP43. Rabbit IgG Aff - Purified Chimp, Hu, Orang-Utan E, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

USP18 antibody
Heart: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

CRISPR

Catalog No. Species Donor Vector  

USP18 - human gene knockout kit via CRISPR
USP18 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

USP18 - human gene knockout kit via CRISPR
USP18 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

USP18 - human gene knockout kit via CRISPR
USP18 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

USP18 - human gene knockout kit via CRISPR
USP18 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

USP18 overexpression lysate
USP18 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

7 Item(s)

per page

Available Filters

Product Type
  1. CRISPR (4)
  2. Lysate (1)
  3. Primary Antibody (2)
Clonality
  1. Polyclonal (2)
Target
  1. USP18 (2)
Property
  1. 40-49 (1)
Reactivity
  1. Human (Hu) (2)
  2. Chimpanzee (Chimp) (1)
  3. Orang-Utan (1)
Applications
  1. Enzyme Immunoassay (E) (2)
  2. Western blot / Immunoblot (WB) (2)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
  4. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (2)
Host
  1. Rabbit (2)
Images
  1. 1 image (2)
Images: Applications
  1. Paraffin Sections (P) (1)
  2. Western blot / Immunoblot (WB) (1)
Species (CRISPR)
  1. Human (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (7)
Service
  • OriGene Technologies GmbH
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