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USP22 antibody

Principal name

USP22 antibody

Alternative names for USP22 antibody

Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, USP3L, Ubiquitin thioesterase 22, Ubiquitin-specific-processing protease 22, Deubiquitinating enzyme 22

SwissProt ID

P0C8Z3 (Bovin), Q5DU02 (Mouse), Q9UPT9 (Human)

Gene ID

23326 (USP22), 216825 (Usp22)

Ncbi ID

9606, NP_056091, NM_015276, NP_056091.1, NP_001004143.2, NM_001004143

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Ze (Zebrafish), African clawed frog, Can (Canine), Eq (Equine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of USP22 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

General readings

Zhang,X.Y., (2008) Mol. Cell 29 (1), 102-111

The USP22 polyclonal antibody ( Cat # PAB4209 ) is used in Western blot to detect USP22 in mouse brain tissue lysate .

PAB4209

Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP22 polyclonal antibody ( Cat # PAB4210 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .

PAB4210

The USP22 polyclonal antibody ( Cat # PAB4210 ) is used in Western blot to detect USP22 in mouse brain tissue lysate .

PAB4210

Western Blot analysis of anti-USP22 (N-term) Pab (Cat.#AP12041PU-N) to detect USP22 in mouse brain tissue lysate.

AP12041PU-N

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AP12042PU-N

Western Blot analysis of anti-USP22 (C-term) Pab (Cat.#AP12042PU-N) to detect USP22 in mouse brain tissue lysate.

AP12042PU-N

Human Heart; WB Suggested Anti-USP22 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; USP22 antibody - N-terminal region (ARP58352_P050) in Human Heart cells using Western Blot

ARP58352_P050

Hum. Fetal Heart; Host: Rabbit . Target Name: USP22 . Sample Tissue: Human Fetal Heart . Antibody Dilution: 1.0ug/ml.; USP22 antibody - middle region (ARP58353_P050) in Hum. Fetal Heart cells using Western Blot

ARP58353_P050

Hum. Fetal Liver; Host: Rabbit . Target Name: USP22 . Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; USP22 antibody - middle region (ARP58353_P050) in Hum. Fetal Liver cells using Western Blot

ARP58353_P050

Hum. Fetal Lung; Host: Rabbit . Target Name: USP22 . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; USP22 antibody - middle region (ARP58353_P050) in Hum. Fetal Lung cells using Western Blot

ARP58353_P050

Human Heart; WB Suggested Anti-USP22 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human heart; USP22 antibody - middle region (ARP58353_P050) in Human Heart cells using Western Blot

ARP58353_P050

Western Blot: USP22 Antibody - Western blot analysis of Usp22 in HeLa whole cell extracts.

TA309926

USP22 Antibody (C-term)(Cat. #TA324831) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the USP22 antibody detected the USP22 protein (arrow).

TA324831

WB Suggested Anti-USP22 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human heart

TA344368

Host: Rabbit; Target Name: USP22; Sample Tissue: Human Fetal Heart; Antibody Dilution: 1.0 ug/ml

TA344368

Host: Rabbit; Target Name: USP22; Sample Tissue: Human Fetal Liver; Antibody Dilution: 1.0 ug/ml

TA344368

Host: Rabbit; Target Name: USP22; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0 ug/ml

TA344368

WB Suggested Anti-USP22 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart

TA344369

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA309926	USP22 antibody
	Rabbit Polyclonal USP22 Antibody
	Rabbit			Purified	Hu, Ms	WB	0.1 ml / €415.00
							OriGene Technologies, Inc.
TA324831	USP22 antibody
	Mouse monoclonal USP22 Antibody (C-term)
	Mouse	IgG1	1154CT13.1.3.1	Purified	Hu	WB	0.4 ml / €415.00
							OriGene Technologies, Inc.
AP12041PU-N	USP22 (N-term) antibody
	Rabbit	Ig		Purified	Hu, Ms	WB	0.4 ml / €415.00
							OriGene Technologies GmbH
AP12042PU-N	USP22 (C-term) antibody
	Rabbit	Ig		Purified	Hu, Ms	P, WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.
LY414666	USP22 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR208422L2V	Lenti ORF particles, Usp22 (GFP-tagged) - Mouse ubiquitin specific peptidase 22 (Usp22), 200 uL, >10^7 TU/mL
		200 µl / €1,220.00
		OriGene Technologies, Inc.

MR208422L1V	Lenti ORF particles, Usp22 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 22 (Usp22), 200 uL, >10^7 TU/mL
		200 µl / €1,090.00
		OriGene Technologies, Inc.

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