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USP3 antibody

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Principal name

USP3 antibody

Alternative names for USP3 antibody

Ubiquitin carboxyl-terminal hydrolase 3, Ubiquitin thioesterase 3, Ubiquitin-specific-processing protease 3, Deubiquitinating enzyme 3

SwissProt ID

Q91W36 (Mouse), Q9Y6I4 (Human)

Gene ID

9960 (USP3), 235441 (Usp3)

Ncbi ID

9606, NP_006528, 5874, NP_006528.2, NP_659186.1, NM_144937, NM_006537

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of USP3 antibody

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

General readings

Puente,X.S., et al., (2003) Nat. Rev. Genet. 4 (7), 544-558

H00009960-M01 - USP3
Western Blot analysis of USP3 expression in transfected 293T cell line by USP3 monoclonal antibody (M01), clone 1H2.Lane 1: USP3 transfected lysate(58.9 KDa).Lane 2: Non-transfected lysate.
H00009960-M01 - USP3
Detection limit for recombinant GST tagged USP3 is approximately 0.3ng/ml as a capture antibody.
ARP33680_P050 - USP3
Human Jurkat; WB Suggested Anti-USP3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. USP3 is supported by BioGPS gene expression data to be expressed in Jurkat.; USP3 antibody - N-terminal region (ARP33680_P050) in Human Jurkat cells using Western Blot
H00009960-B01 - USP3
Immunoperoxidase of purified MaxPab antibody to USP3 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml]
H00009960-B01 - USP3
Western Blot analysis of USP3 expression in transfected 293T cell line (H00009960-T01) by USP3 MaxPab polyclonal antibody.Lane 1: USP3 transfected lysate(57.2 KDa).Lane 2: Non-transfected lysate.
PAB3917 - USP3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP3 polyclonal antibody ( Cat # PAB3917 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PAB3918 - USP3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with USP3 polyclonal antibody ( Cat # PAB3918 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
PAB3918 - USP3
The USP3 polyclonal antibody ( Cat # PAB3918 ) is used in Western blot to detect USP3 in A-375 cell lysate.
H00009960-D01P - USP3
USP3 MaxPab rabbit polyclonal antibody. Western Blot analysis of USP3 expression in mouse spleen.
H00009960-D01P - USP3
Western Blot analysis of USP3 expression in transfected 293T cell line (H00009960-T03) by USP3 MaxPab polyclonal antibody.Lane 1: USP3 transfected lysate(58.90 KDa).Lane 2: Non-transfected lysate.
H00009960-D01P - USP3
USP3 MaxPab rabbit polyclonal antibody. Western Blot analysis of USP3 expression in Hela NE.
AP12006PU-N - USP3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP12006PU-N - USP3
Western blot analysis of anti-USP3 Antibody (N-term) (Cat.#AP12006PU-N) in 293 cell line lysates (35ug/lane). USP3 (arrow) was detected using the purified Pab.
AP12007PU-N - USP3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP12007PU-N - USP3
Western blot analysis of anti-USP3 Antibody (C-term) (Cat.#AP12007PU-N) in K562 cell line lysates (35ug/lane). USP3 (arrow) was detected using the purified Pab.
ARP33681_P050 - USP3
Human Jurkat; WB Suggested Anti-USP3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate. USP3 is supported by BioGPS gene expression data to be expressed in Jurkat.; USP3 antibody - middle region (ARP33681_P050) in Human Jurkat cells using Western Blot
TP309550 - USP3
TA334220 - USP3
WB Suggested Anti-USP3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateUSP3 is supported by BioGPS gene expression data to be expressed in Jurkat
TA334221 - USP3
WB Suggested Anti-USP3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Jurkat cell lysateUSP3 is supported by BioGPS gene expression data to be expressed in Jurkat

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

USP3 (N-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

USP3 (C-term) antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

USP3
  Recombinant protein of human ubiquitin specific peptidase 3 (USP3)  
USP3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Usp3 (GFP-tagged) - Mouse ubiquitin specific peptidase 3 (Usp3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Usp3 (GFP-tagged) - Mouse ubiquitin specific peptidase 3 (Usp3), 200 uL, >10^7 TU/mL  
200 µl / €1,220.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Usp3 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 3 (Usp3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Usp3 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 3 (Usp3), 200 uL, >10^7 TU/mL  
200 µl / €1,090.00
  OriGene Technologies, Inc.
 

5 Item(s)

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Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (2)
  3. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (2)
Target
  1. USP3 (3)
Property
  1. C-term (1)
  2. N-term (1)
Reactivity
  1. Human (Hu) (2)
Applications
  1. Paraffin Sections (P) (2)
  2. Western blot / Immunoblot (WB) (2)
Presentation
  1. Purified (2)
Host
  1. Rabbit (2)
Images
  1. 1 image (3)
Images: Applications
  1. Paraffin Sections (P) (2)
  2. Western blot / Immunoblot (WB) (2)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (5)
Service
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