USP30 antibody
Principal name
USP30 antibody
Alternative names for USP30 antibody
Ubiquitin carboxyl-terminal hydrolase 30, Ubiquitin thioesterase 30, Ubiquitin-specific protease 30, Deubiquitinating enzyme 30
SwissProt ID
Q70CQ3 (Human)
Gene ID
84749 (USP30)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of USP30 antibody
- Kit Component:
- - KN202538G1, USP30 gRNA vector 1 in pCas-Guide vector
- KN202538G2, USP30 gRNA vector 2 in pCas-Guide vector
- KN202538D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Human HeLa; USP30 antibody - middle region (ARP50098_P050) validated by WB using Hela cell lysate; USP30 antibody - middle region (ARP50098_P050) in Human HeLa cells using Western Blot
Human THP1; WB Suggested Anti-USP30 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: THP-1 cell lysate; USP30 antibody - middle region (ARP50098_P050) in Human THP1 cells using Western Blot
USP30 Antibody (N-term) (AP54489PU-N) western blot analysis in A549 cell line lysates (35 µg/lane). This demonstrates the USP30 antibody detected the USP30 protein (arrow).
Immunofluorescent analysis of USP30 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of USP30 expression in A549 (A); HeLa (B); THP1 (C) whole cell lysates.
Western blot analysis of extracts from HeLa cells, using USP30 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-USP30 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: THP-1 cell lysate
USP30 antibody - middle region validated by WB using Hela cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315935 | USP30 antibody |
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| Rabbit polyclonal anti-USP30 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP54489PU-N | USP30 (N-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||