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UVRAG antibody

Principal name

UVRAG antibody

Alternative names for UVRAG antibody

p63, UV radiation resistance-associated gene protein

SwissProt ID

Q9P2Y5 (Human)

Gene ID

7405 (UVRAG)

Ncbi ID

9606, NP_003360.2, NM_003369

Available reactivities

Hu (Human), Hst (Hamster), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of UVRAG antibody

Kit Component:: - KN209428G1, UVRAG gRNA vector 1 in pCas-Guide vector
- KN209428G2, UVRAG gRNA vector 2 in pCas-Guide vector
- KN209428D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:: CRISPR/CAS9 Genome Editing Manual

Western Blot analysis of UVRAG expression in transfected 293T cell line (H00007405-T01) by UVRAG MaxPab polyclonal antibody.Lane 1: UVRAG transfected lysate(76.89 KDa).Lane 2: Non-transfected lysate.

H00007405-B01

Western Blot analysis of UVRAG expression in transfected 293T cell line (H00007405-T02) by UVRAG MaxPab polyclonal antibody.Lane 1: UVRAG transfected lysate(76.89 KDa).Lane 2: Non-transfected lysate.

H00007405-B01P

Western blot analysis of UVRAG expression in HeLa (1), Jurkat (2), NIH/3T3 (3), WR19L (4), Rat1 (5) and CHO (6) using AM26654AF-N.

AM26654AF-N

Immunoprecipitation of UVRAG from HeLa (1, 2) and NIH/3T3 (3, 4) with mouse IgG1 (1, 3) or AM26654AF-N (2, 4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblottedwith AM26654AF-N.

AM26654AF-N

ELISA: UVRAG Antibody

TA590654

Immunoprecipitation and western blot analysis of anti-UVRAG Pab (Cat.#TA324649) in 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with mouse UVRAG (Lane 1). Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4). Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.

TA324649

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TA324649

Immunofluorescence staining of Autophagy UVRAG antibody (Cat# TA324649) on Methanol-fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.

TA324649

IF image of U251 cells stained with UVRAG (C-term) antibody. U251 cells were treated with Chloroquine , then incubated with TA324649 UVRAG (C-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . UVRAG immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

TA324649

Western Blot: UVRAG Antibody [TA336981] - Analysis of UVRAG in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart using this antibody at 0 ug/ml, 0.5 ug/ml and 0.5 ug/ml, respectively.

TA336981

Western blot analysis of UVRAG in mouse lung tissue lysate with UVRAG antibody at 1 ug/mL.

TA349093

Immunohistochemistry of UVRAG in human lung tissue with UVRAG antibody at 5 ug/mL.

TA349093

Immunofluorescence of UVRAG in human lung tissue with UVRAG antibody at 20 ug/mL.

TA349093

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA324649	UVRAG (C-term) antibody
	Rabbit polyclonal UVRAG Antibody (C-term)
	Rabbit	Ig		Purified	Hu, Ms	ICC/IF, IP, P, WB	0.4 ml / €415.00
							OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
TA336981	UVRAG antibody
	Rabbit Polyclonal UVRAG Antibody
	Rabbit			Purified	Can, Chk, Hu, Ms, Prim, Rt	WB	0.1 mg / €335.00
							OriGene Technologies, Inc.
TA349093	UVRAG antibody
	Rabbit Polyclonal Anti-UVRAG Antibody
	Rabbit	IgG		Purified	Hu, Ms	ICC/IF, P, WB	0.1 mg / €415.00
							OriGene Technologies, Inc.
+2 additional images
TA590654	UVRAG antibody
	Rabbit Polyclonal UVRAG Antibody
	Rabbit	IgG		Purified	Hu	E	50 µg / €409.00
							OriGene Technologies, Inc.
AM26654AF-N	UVRAG (389-699) antibody
	Mouse	IgG2a	1H4	Azide Free	Hst, Hu, Ms, Rt	IP, WB	0.1 mg / €340.00
							OriGene Technologies GmbH
+1 additional image