Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » VDAC1 antibody

VDAC1 antibody

(reset)

Principal name

VDAC1 antibody

Alternative names for VDAC1 antibody

VDAC, Porin 31HL, Porin 31HM, Mitochondria marker, Voltage-dependent anion-selective channel protein 1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin

SwissProt ID

P21796 (Human), P45879 (Bovin), Q60932 (Mouse), Q9MZ16 (Pig), Q9TT15 (Rabit), Q9Z2L0 (Rat)

Gene ID

7416 (VDAC1), 83529 (Vdac1), 22333

Ncbi ID

NP_003365.1, NP_003365, 9606, NP_112643.1, NP_035824.1, NM_031353, NM_011694

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Av (Birds), Mam (Mammalia), Re (Reptiles)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of VDAC1 antibody

Voltage-dependent anion channel (VDAC) is an outer mitochondrial membrane channel protein expressed in a ubiquitous manner as it is required for proper mitochondrial function. The three isoforms expressed in higher eukaryotes are highly conserved. VDAC1, VDAC2 and VDAC3 allow the entry and exit of most metabolites in and out of the mitochondria. The channel spans the membrane 19 times through antiparallel b strands. Studies show that VDAC channels exhibit ion selectivity and are dependent on voltage. When in the open state, VDAC channels conduct mainly organic anions such as ATP, ADP and inorganic phosphate (Pi). In the closed state they still conduct ions, but much smaller and mostly inorganic like K+, + and Ca2+ . However, while cells are metabolically active, VDAC channels are constitutively open in order to eble oxidative phosphorylation in the mitochondria to take place (through the passage of ADP across the membrane). VDAC channels are best described for their role in apoptosis. In the early stages of the sigling pathway, a proapoptotic factor, a member of the Bcl2 family is responsible for closing the channel thereby decreasing the release of ADP and ATP from the mitochondria. VDAC channels are also implicated in the Warburg effect, which is typical to cancer cell. In such cells, hexokise (the first enzyme in the glycolytic pathway responsible for phosphorylating glucose on C6 to yield glucose-6 phosphate) known to be up-regulated, binds to VDAC1 and takes up most of the ATP released by the channel in order to phosphorylate glucose. This action speeds up glycolysis if downstream reactions function properly. If however a glycolysis step is inhibited or down-regulated, glucose-6-phosphate builds up and consequently inhibits hexokise, thereby restoring proper mitochondrial function. VDAC is also detected in the plasma membrane of various cell types including lymphocytes, epithelial cells, and astrocytes. The postulated roles for VDAC in the plasma membrane mainly involve the regulation of cellular ATP release and volume control. VDAC have also been proposed to function as the plasma membrane maxi anion channels. None of the isoforms are essential for viability. Knock out of VDAC3 in mice causes sterility in males. That of VDAC1 and VDAC2 affects breathing and the individual knock out of VDAC1 causes embryonic death in mice in some cases.

General readings

1. Blachly-Dyson, E., Zambronicz, E. B., Yu, W. H., Adams, V., McCabe, E. R. B., Adelman, J.,
Colombini, M., Forte, M.Cloning and functional expression in yeast of two human isoforms of the
outer mitochondrial membrane channel, the voltage-dependent anion channel.J. Biol. Chem. 268:
1835-1841, 1993.
2. Geisler, S., Holmstrom, K. M., Skujat, D., Fiesel, F. C., Rothfuss, O. C., Kahle, P. J., Springer,
W.PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1.Nature Cell Biol.
12: 119-131, 2010.
3. Huizing, M., Ruitenbeek, W., van den Heuvel, L. P., Dolce, V., Iacobazzi, V., Smeitink, J. A. M.,
Palmieri, F., Trijbels, J. M. F.Human mitochondrial transmembrane metabolite carriers: tissue
distribution and its implication for mitochondrial disorders.J. Bioenerg. Biomembr. 30: 277-284,
1998.

AP00265PU-N - VDAC1
Western blot analysis of VDAC/Porin with 3T3 cell lysate.
ARP35122_T100 - VDAC1
Human Liver; Rabbit Anti-VDAC1 Antibody. Catalog Number: ARP35122. Paraffin Embedded Tissue: Human Liver. Cellular Data: Hepatocytes. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; VDAC1 antibody - C-terminal region (ARP35122_T100) in Human Liver cells using Immunohistochemistry
ARP35122_T100 - VDAC1
Human; . Lanes: Lane 1: 50ug HeLa lysate. Lane 2: 50ug 293T lysate. Lane 3: 50ug K562 lysate. Lane 4: 50ug MDA-MB-231 lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:1000. Gene Name: VDAC1. Submitted by: David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences .; VDAC1 antibody - C-terminal region (ARP35122_T100) in Human cells using Western Blot
ARP35122_T100 - VDAC1
Human HepG2; WB Suggested Anti-VDAC1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. VDAC1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; VDAC1 antibody - C-terminal region (ARP35122_T100) in Human HepG2 cells using Western Blot
AP07648PU-N - VDAC1
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver.
PAB1231 - VDAC1
Immunoblot analysis of cell lysates. Lane 1 : Hela cell lysate and lane 2 : HepG2 cell lysate. ( * N.S : Nonspecific band )
H00007416-M05 - VDAC1
Detection limit for recombinant GST tagged VDAC1 is approximately 1ng/ml as a capture antibody.
PAB11290 - VDAC1
Western blot using VDAC1 polyclonal antibody ( Cat # PAB11290 ) detects a single band at ~32 kDa corresponding to VDAC1 ( arrowhead ) present in a rat heart tissue lysate.Approximately 35 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.After blocking, the membrane was probed with the primary antibody diluted to 1 : 1,200 at 4°C overnight.Washes were followed by reaction with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
PAB9069 - VDAC1
Western blot analysis of VDAC1 expression in NIH/3T3 cell lysate.
AP17825PU-N - VDAC1
Western blot analysis of VDAC1 Antibody (N-term) (AP17825PU-N) in A375 cell line and mouse heart tissue lysates (35ug/lane). VDAC1 (arrow) was detected using the purified Pab.
AM31934PU-N - VDAC1
Human Brain Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31934PU-N - VDAC1
Detection limit for recombinant GST tagged VDAC1 is 3 ng/ml as a capture antibody.
75-204 - VDAC1
Adult rat hippocampus immunohistochemistry
75-204 - VDAC1
Immunoblot versus membranes from livers of adult wild-type (WT) and VDAC1 knockout (KO), brains of WT mice and brains of adult rat brain (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).
75-204 - VDAC1
Adult rat brain membrane immunoblot
75-204 - VDAC1
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N152B/23 (left) orN52A/42 (right) TC supe.
73-204 - VDAC1
Adult rat hippocampus immunohistochemistry
73-204 - VDAC1
Immunoblot versus membranes from livers of adult wild-type (WT) and VDAC1 knockout (KO), brains of WT mice and brains of adult rat brain (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).
73-204 - VDAC1
Adult rat brain membrane immunoblot
73-204 - VDAC1
Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N152B/23 (left) orN52A/42 (right) TC supe.
TA308959 - VDAC1
Sample (30 ug of whole cell lysate). A: A431 . 12% SDS PAGE. VDAC1 antibody. TA308959 diluted at 1:1000.
TA308959 - VDAC1
Western blot analysis of extracts (10ug) from 7 different cell lines by using anti- VDAC1 Polyclonal antibody.(1:1000)
TA308959 - VDAC1
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using VDAC1(TA308959) antibody at 1:500 dilution.
TA308959 - VDAC1
VDAC1 antibody [N1C2] detects VDAC1 protein at mitochondria on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. VDAC1 antibody [N1C2] (TA308959) dilution: 1:500.
TA319257 - VDAC1
Western Blot of Rabbit Anti-VDAC/Porin Antibody. Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead). Other band(s): none.
TA322858 - VDAC1
Predicted band size: 30 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds)
TA322858 - VDAC1
Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using VDAC1 antibody at dilution 1/13, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA326858 - VDAC1
Western blot analysis of extracts of various cell lines, using VDAC1 antibody.
TA326858 - VDAC1
Immunohistochemistry of paraffin-embedded human stomach cancer using VDAC1 antibody at dilution of 1:200 (400x lens).
TA326858 - VDAC1
Immunofluorescence analysis of HeLa cell using VDAC1 antibody. Blue: DAPI for nuclear staining.
TA329065 - VDAC1
Western blot analysis of rat kidney (lanes 1 and 4), brain (lanes 2 and 5) mouse brain (lanes 3 and 6) membrane and CCF-SSTGI (lanes 7 and 8) cell line lysate: 1-3, 7. Anti-Pan Voltage Dependent Anion Channels antibody, (1:200). 4-6, 8. Anti-Pan Voltage Dependent Anion Channels antibody, preincubated with the control peptide antigen.
  1. Quick-Filters:
  2. Highly Validated (4)

17 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VDAC1 antibody
  Rabbit polyclonal anti-VDAC1/Porin antibody, Loading control  
Sample (30 ug of whole cell lysate). A: A431 . 12% SDS PAGE. VDAC1 antibody. TA308959 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

VDAC1 antibody
Adult rat hippocampus immunohistochemistry Mouse IgG2a N152B/23 Supernatant Hu, Ms, Rt C, ICC/IF, WB
5 ml / €490.00
  Antibodies Incorporated
+3 additional images

Highly validated antibody!

NeuroMab

VDAC1 antibody
Adult rat hippocampus immunohistochemistry Mouse IgG2a N152B/23 Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €570.00
  Antibodies Incorporated
+3 additional images

Highly validated antibody!

NeuroMab

VDAC1 (N-term) antibody
Western blot analysis of VDAC1 Antibody (N-term) (AP17825PU-N) in A375 cell line and mouse heart tissue lysates (35ug/lane). VDAC1 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu, Ms F, P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+4 additional images

Highly validated antibody!

VDAC1 (185-197) (Loading Control) antibody
  Rabbit polyclonal VDAC/Porin antibody  
Western Blot of Rabbit Anti-VDAC/Porin Antibody. Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead). Other band(s): none. Rabbit IgG Aff - Purified Av, Hu, Mam, Re E, WB
0.2 mg / €450.00
  OriGene Technologies, Inc.

VDAC1 antibody
  Anti-VDAC1 Rabbit Polyclonal Antibody  
Predicted band size: 30 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

VDAC1 antibody
  VDAC1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using VDAC1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+2 additional images

VDAC1 antibody
  Rabbit polyclonal Anti-Pan Voltage Dependent Anion Channels Antibody  
Western blot analysis of rat kidney (lanes 1 and 4), brain (lanes 2 and 5) mouse brain (lanes 3 and 6) membrane and CCF-SSTGI (lanes 7 and 8) cell line lysate: 1-3, 7. Anti-Pan Voltage Dependent Anion Channels antibody, (1:200). 4-6, 8. Anti-Pan Voltage Dependent Anion Channels antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €585.00
  OriGene Technologies, Inc.

VDAC1 (3594-100) antibody
Western blot analysis of VDAC/Porin with 3T3 cell lysate. Rabbit Purified Bov, Hu, Ms, Por, Rb, Rt C, WB
0.1 mg / €395.00
  OriGene Technologies GmbH

VDAC1 antibody
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver. Rabbit Aff - Purified Hu E, P, WB
50 µg / €425.00
  OriGene Technologies GmbH

CRISPR

Catalog No. Species Donor Vector  

VDAC1 - human gene knockout kit via CRISPR
VDAC1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

VDAC1 - human gene knockout kit via CRISPR
VDAC1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

VDAC1 - human gene knockout kit via CRISPR
VDAC1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

VDAC1 - human gene knockout kit via CRISPR
VDAC1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Vdac1 (GFP-tagged) - Mouse voltage-dependent anion channel 1 (Vdac1), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL
Lenti ORF particles, Vdac1 (GFP-tagged) - Mouse voltage-dependent anion channel 1 (Vdac1), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Vdac1 (GFP-tagged ORF) - Rat voltage-dependent anion channel 1 (Vdac1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Vdac1 (GFP-tagged ORF) - Rat voltage-dependent anion channel 1 (Vdac1), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Vdac1 (Myc-DDK-tagged ORF) - Rat voltage-dependent anion channel 1 (Vdac1), 200 uL, >10^7 TU/mL
Lenti ORF particles, Vdac1 (Myc-DDK-tagged ORF) - Rat voltage-dependent anion channel 1 (Vdac1), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

17 Item(s)

per page
17 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (4)
  2. Mouse Lenti ORF Particle (1)
  3. Primary Antibody (10)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (8)
  2. Monoclonal (2)
Target
  1. VDAC1 (10)
Property
  1. 185-197 (1)
  2. 3594-100 (1)
  3. Loading Control (1)
  4. N-term (1)
Reactivity
  1. Human (Hu) (9)
  2. Mouse (Ms) (7)
  3. Rat (Rt) (6)
  4. Birds (Av) (1)
  5. Bovine (Bov) (1)
  6. Mammalia (Mam) (1)
  7. Porcine (Por) (1)
  8. Rabbit (Rb) (1)
  9. Reptiles (Re) (1)
Applications
  1. Western blot / Immunoblot (WB) (9)
  2. Paraffin Sections (P) (4)
  3. Frozen sections (C) (3)
  4. Enzyme Immunoassay (E) (3)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  6. Flow cytometry (F) (1)
Presentation
  1. Purified (6)
  2. Aff - Purified (3)
  3. Supernatant (1)
Host
  1. Rabbit (8)
  2. Mouse (2)
Images
  1. 1 image (10)
  2. 3+ images (5)
  3. 5+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (9)
  2. IHC (3)
  3. Frozen sections (C) (2)
  4. Paraffin Sections (P) (2)
  5. Flow cytometry (F) (1)
  6. Immunofluorescence (IF) (1)
Species (CRISPR)
  1. Human (4)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. RFP-BSD (1)
  4. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (15)
  2. Antibodies Incorporated (2)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn