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VEGF-B antibody

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Principal name

VEGF-B antibody

Alternative names for VEGF-B antibody

VEGFB, VRF, Vascular endothelial growth factor B, VEGF-related factor

SwissProt ID

O35485 (Rat), P49765 (Human), P49766 (Mouse), Q9XS49 (Bovin)

Gene ID

7423 (VEGFB), 22340 (Vegfb)

Ncbi ID

9606, NP_003368.1, NP_003368, 10090, NP_001172093.1, NM_011697, NM_003377

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Mky (Monkey)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of VEGF-B antibody

Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity (1, 2). Five mammalian members, including VEGF-A, B, C, D and PlGF, have been identified. VEGF family members are disulfide-linked dimeric proteins that are important regulators of physiological and pathological vasculogenesis, angiogenesis and lymphangiogenesis. VEGF-B is expressed in most tissues, especially in heart, skeletal muscle and pancreas. In many tissues, VEGF-B is coexpressed and can heterodimerize with VEGF (3). By alternative splicing, two isoforms of mature VEGF-B containing 167 or 186 amino acid (aa) residues exist (3, 4). The two VEGF-B isoforms have identical amino-terminal cysteine knot VEGF homology domains but the carboxyl end of VEGF-B167 differs from that of VEGF-B186 by the presence of a highly basic cysteine-rich heparin binding domain. Whereas VEGF-B186 is a secreted diffusible protein, VEGF-B167 is sequestered into the cell matrix after secretion. Both VEGF-B isoforms bind VEGF receptor 1 (VEGFR-1), but not VEGFR-2 or VEGFR-3 (5). On endothelial cells, ligation of VEGFR-1 by VEGF-B has been shown to regulate the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1. VEGF-B167 and a proteolytically processed form of VEGF-B186 (VEGF-B127) also bind neuropilin1 (NP1), a type I transmembrane receptor for semaphorins/collapsins, ligands involved in neuron guidance (6). Besides VEGF-B, NP1 has been shown to bind PlGF-2, VEGF165 and VEGFR-1 (6, 7). The many interactions of NP1 with VEGF ligands and receptor suggest that NP1 may function as a regulator of angiogenesis (7).

General readings

Claesson-Welsh L. (2008) Arterioscler Thromb Vasc Biol. 28(9):1575-6.
Malik AK,, et al. (2006) Blood. 107(2):550-7.

AP06477PU-N - VEGF-B
Western blot (WB) analysis of VEGFB antibody (Cat.-No.: AP06477PU-N) in extracts from Jurkat cells.
H00007423-B01P - VEGF-B
Western Blot analysis of VEGFB expression in transfected 293T cell line (H00007423-T01) by VEGFB MaxPab polyclonal antibody.Lane 1: VEGFB transfected lysate(22.77 KDa).Lane 2: Non-transfected lysate.
AR09129PU-L - VEGF-B
AR09129PU-L - VEGF-B
Reombinant human VEGF-B, aa 22-207, His-tagged: 15% SDS-PAGE (3 µg)
H00007423-D01P - VEGF-B
Proximity Ligation Analysis (PLA) of protein-protein interactions between VEGFB and VEGFA. HeLa cells were stained with anti-VEGFB rabbit purified polyclonal 1:1200 and anti-VEGFA mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00007423-D01P - VEGF-B
Western Blot analysis of VEGFB expression in transfected 293T cell line (H00007423-T01) by VEGFB MaxPab polyclonal antibody.Lane 1: VEGFB transfected lysate(21.60 KDa).Lane 2: Non-transfected lysate.
H00007423-D01 - VEGF-B
Immunoprecipitation of VEGFB transfected lysate using anti-VEGFB MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with VEGFB MaxPab mouse polyclonal antibody (B01) (H00007423-B01).
C0389-1 - VEGF-B
Western blot analysis of extracts from Jurkat cells, using VEGFB Antibody. The lane on the right is treated with the synthesized peptide.
ARP58975_P050 - VEGF-B
Human ACHN; WB Suggested Anti-VEGFB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: ACHN cell lysate. VEGFB is supported by BioGPS gene expression data to be expressed in ACHN.; VEGFB antibody - middle region (ARP58975_P050) in Human ACHN cells using Western Blot
ARP58976_P050 - VEGF-B
Human HT1080; WB Suggested Anti-VEGFB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate; VEGFB antibody - C-terminal region (ARP58976_P050) in Human HT1080 cells using Western Blot
CPA2220-100ul - VEGF-B
Immunohistochemical analysis of VEGFB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA2220-100ul - VEGF-B
Western blot analysis of VEGFB expression in HT1080 (A); ACHN (B) whole cell lysates.
TA507194 - VEGF-B
Anti-VEGFB mouse monoclonal antibody (TA507194) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VEGFB(RC202426).
TA507194 - VEGF-B
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFB (RC202426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFB.
TA507196 - VEGF-B
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VEGFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507196)
TA507196 - VEGF-B
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFB (RC202426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFB.
TA507205 - VEGF-B
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VEGFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507205)
TA507205 - VEGF-B
Anti-VEGFB mouse monoclonal antibody (TA507205) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VEGFB(RC202426).
TA507205 - VEGF-B
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFB (RC202426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFB.
TA590656 - VEGF-B
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFB (RC202426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFB.
TA590656 - VEGF-B
ELISA: VEGFB Antibody
TP302426 - VEGF-B
TP723473 - VEGF-B
TA314667 - VEGF-B
Western blot analysis of extracts from Jurkat cells, using VEGFB antibody.The lane on the right is treated with the synthesized peptide.
TA314667 - VEGF-B
Western blot analysis of extracts from K562 cells (Lane 2) and 293 cells (Lane 3), using VEGFB Antibody . The lane on the left is treated with synthesized peptide.
TA322859 - VEGF-B
Predicted band size: 21 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/700 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds)
TA322859 - VEGF-B
Predicted cell location: Cytoplasm. Positive control: Human thyroid cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using VEGFB antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA325971 - VEGF-B
Western blot analysis of VEGFB expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA327289 - VEGF-B
Western blot analysis of extracts of mouse heart cell lines, using VEGFB antibody.
TA327289 - VEGF-B
Immunohistochemical of paraffin-embedded human endometrial cancer using VEGFB antibody at dilution of 1:200 (200x lens).
TA327289 - VEGF-B
Immunohistochemical of paraffin-embedded human Neurilemmoma using VEGFB antibody at dilution of 1:200 (200x lens).
TA347938 - VEGF-B
Western blot analysis of VEGFB expression in Jurkat cells

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

VEGF-B antibody
  Rabbit polyclonal anti-VEGFB antibody  
Western blot analysis of extracts from Jurkat cells, using VEGFB antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

VEGF-B antibody
  Anti-VEGFB Rabbit Polyclonal Antibody  
Predicted band size: 21 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/700 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

VEGF-B antibody
  Rabbit Polyclonal VEGFB antibody  
Western blot analysis of VEGFB expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

VEGF-B antibody
  VEGFB Rabbit Polyclonal Antibody  
Western blot analysis of extracts of mouse heart cell lines, using VEGFB antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+2 additional images

VEGF-B antibody
  Rabbit Polyclonal Anti-VEGFB Antibody  
Western blot analysis of VEGFB expression in Jurkat cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €335.00
  OriGene Technologies, Inc.

VEGF-B antibody
  VEGFB mouse monoclonal antibody, clone OTI2D6 (formerly 2D6)  
Anti-VEGFB mouse monoclonal antibody (TA507194) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VEGFB(RC202426). Mouse IgG1 OTI2D6 Purified Hu ICC/IF, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

VEGF-B antibody
  VEGFB mouse monoclonal antibody, clone OTI2C4 (formerly 2C4)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VEGFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507196) Mouse IgG2b OTI2C4 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

VEGF-B antibody
  VEGFB mouse monoclonal antibody, clone OTI1H9 (formerly 1H9)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VEGFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507205) Mouse IgG1 OTI1H9 Purified Hu ICC/IF, P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+2 additional images

TrueMAB

VEGF-B antibody
  Rabbit Polyclonal VEGFB Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFB (RC202426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFB. Rabbit IgG Purified Hu E, WB
0.1 mg / €409.00
  OriGene Technologies, Inc.
+1 additional image

VEGF-B antibody
Western blot (WB) analysis of VEGFB antibody (Cat.-No.: AP06477PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

VEGF-B (VEGF-B167) antibody
VEGF-B Rabbit IgG Purified Hu WB
0.2 mg / €370.00
  OriGene Technologies GmbH

VEGF-B (VEGF-B167) antibody
VEGF-B Rabbit IgG Purified Hu WB
0.1 mg / €270.00
  OriGene Technologies GmbH

VEGF-B (C-term) antibody
Immunohistochemical staining of paraffin-embedded rat cardiac muscle tissue, using Anti-VEGF-B. Bottom: Detection of VEGF-B from rat cardiac muscle tissue lysate in Western blot assay, using Anti- VEGF-B. Rabbit IgG Aff - Purified Hu, Ms, Rb, Rt P, WB
0.1 ml / €460.00
  Neuromics Antibodies

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

VEGF-B
  Recombinant protein of human vascular endothelial growth factor B (VEGFB)  
VEGF-B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

VEGF-B
  Purified recombinant protein of Human vascular endothelial growth factor B (VEGFB).  
VEGF-B Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.		 E. coli
20 µg / €230.00
  OriGene Technologies, Inc.
 

15 Item(s)

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15 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (13)
  2. Protein | Growth Factor (2)
Clonality
  1. Polyclonal (10)
  2. Monoclonal (3)
Target
  1. VEGF-B (15)
Property
  1. C-term (1)
  2. VEGF-B167 (2)
Reactivity
  1. Human (Hu) (13)
  2. Mouse (Ms) (7)
  3. Rat (Rt) (7)
  4. Rabbit (Rb) (1)
Applications
  1. Western blot / Immunoblot (WB) (12)
  2. Paraffin Sections (P) (5)
  3. Enzyme Immunoassay (E) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
Presentation
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  2. Aff - Purified (2)
Host
  1. Rabbit (10)
  2. Mouse (3)
Images
  1. 1 image (13)
  2. 3+ images (2)
Images: Applications
  1. Western blot / Immunoblot (WB) (10)
  2. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  3. IHC (2)
  4. Paraffin Sections (P) (2)
  5. Enzyme Immunoassay (E) (1)
Species (Protein)
  1. Human (2)
Source
  1. E. coli (1)
  2. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (14)
  2. Neuromics Antibodies (1)
Service
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