VGF antibody
Principal name
VGF antibody
Alternative names for VGF antibody
Neurosecretory protein VGF
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of VGF antibody
VGF (VGF, Nerve Growth Factor-Inducible), is a protein and neuropeptide that may play a role in regulating energy homeostasis, metabolism. Canu et al. (1997) noted that rat Vgf encodes a predicted 70-kD polypeptide that shares similarities with the secretogranin/chromogranin family and is found in the secretory granules of subsets of neurons and endocrine cells. By fluorescence in situ hybridization, Canu et al. (1997) assigned the VGF gene to 7q22. Canu et al. (1997) demonstrated that the single-copy human VGF gene spans 6 kb of genomic DNA and contains 2 exons. The entire VGF protein is encoded by exon 2, while exon 1 contains only 5-prime untranslated sequence. The structural organization of the human gene is similar to that described for the rat VGF gene (Salton et al., 1991), and both the translated and the untranslated regions show a high degree of sequence homology to the rat gene.
Immunohistochemistry (IHC) analyzes of VGF antibody (Cat.-No.: AP01489PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of VGF antibody (Cat.-No.: AP01489PU-N) in extracts from RAW264.7 cells.
Immunohistochemistry of VGF in human brain with VGF polyclonal antibody ( Cat # PAB13036 ) at 5 µg/ml.
Western blot analysis of VGF in human brain tissue lysate with VGF polyclonal antibody ( Cat # PAB13036 ) at ( A ) 0.5 and ( B ) 1 µg/ml.
Transfected 293T; WB Suggested Anti-VGF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; VGF antibody - middle region (ARP42127_P050) in Transfected 293T cells using Western Blot
Human THP-1; WB Suggested Anti-VGF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; VGF antibody - middle region (ARP42128_P050) in Human THP-1 cells using Western Blot
Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with VGF antibody AP22596PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from RAW264.7 cells, using VGF Antibody. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of VGF staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of VGF staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of VGF expression in human brain (A); RAW264.7 (B); PC12 (C) whole cell lysates.
Western blot analysis of VGF in human brain tissue lysate with VGF antibody at (A) 0.5 and (B) 1 ug/ml.
Immunohistochemistry of VGF in human brain with VGFantibody at 5 ug/ml.
Immunofluorescence of VGF in Human Brain cells with VGF antibody at 20 ug/mL.
Western blot analysis of extracts from RAW264.7 cells, using VGF antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using VGF antibody.The picture on the right is treated with the synthesized peptide.
Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (VGF Antibody) at dilution 1/500 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 1 minute
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (VGF Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (VGF Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: ×200)
WB Suggested Anti-VGF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate
WB Suggested Anti-VGF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP01489PU-N | VGF antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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| AP22596PU-N | VGF (C-term) antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| AP31225PU-N | VGF (C-term) antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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