Vimentin antibody
Principal name
Vimentin antibody
Alternative names for Vimentin antibody
VIM
SwissProt ID
P02543 (Pig), P08670 (Human), P09654 (Chick), P20152 (Mouse), P31000 (Rat), P48616 (Bovin), P48670 (Crigr), Q92155 (Cypca), Q9MZA9 (Sheep)
Ncbi ID
NP_003371.2, 7962, 9606, NP_003371, NP_776394, NP_776394.2, NP_112402.1, NP_035831.2, NM_011701, NM_031140, NM_003380
Available reactivities
Available hosts
Available applications
Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Frozen Sections (C), Flow Cytometry (F), ELISA (detection) (E(detection)), Luminex (LMNX)
Background of Vimentin antibody
Vimentin is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin . Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with glial filament acidic protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive.
Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma. Gene locus: Human chomosome 10p13.
General readings
1. Lilienbaum, A.; Legagneux, V.; Portier, M.-M.; Dellagi, K.; Paulin, D. : Vimentin gene: expression in human lymphocytes and in Burkitt's lymphoma cells. EMBO J. 5: 2809-2814, 1986.
2. Mor-Vaknin, N.; Punturieri, A.; Sitwala, K.; Markovitz, D. M. : Vimentin is secreted by activated macrophages. Nature Cell Biol. 5: 59-63, 2003
3 Ferrari, S.; Cannizzaro, L. A.; Battini, R.; Huebner, K.; Baserga, R. : The gene encoding human vimentin is located on the short arm of chromosome 10. Am. J. Hum. Genet. 41: 616-626, 1987.
Western blot analysis of Vimentin expression in mouse small intestine tissue lysate.
Figure 1. Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547. Nuclei are stained with DAPI (blue).
Figure 2. Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-01) Dyomics 547. Nuclei are stained with DAPI (blue).
Figure 3. Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4). Lane 1,2: immunostaining with anti-Vimentin (VI-01)Lane 3,4: immunostaining with anti-human Vimentin (VI-RE/1; SM3129P)
Figure 4. Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa.Lr = lysate (reducing conditions)Lnr = lysate (non-reducing conditions)IPr = immunoprecipitate (reducing conditions)IPnr = immunoprecipitate (non-reducing conditions)
Figure 1. Immunofluorescence staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01; BM753S), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).
Figure 2. Immunofluorescence staining of RBL rat basophilic cell line with anti-Vimentin (VI-10) Dyomics 547. Nuclei are stained with DAPI (blue).
Figure 3. Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa.Lr = lysate (reducing conditions)Lnr = lysate (non-reducing conditions)IPr = immunoprecipitate (reducing conditions)IPnr = immunoprecipitate (non-reducing conditions)
Immunohistochemistry on paraffin section of human colon.
Immunofluorescence staining of the developing neural tube in a 2 days old Zebrafish embryo.
Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining
Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining
Immunohistochemistry on Frozen section of swine colon using Vimentin Antibody Cat.-No BM6008P showing positive staining in connective tissue cells and no reactivity in epithelial cells.
Detection of endogenous Vimentin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Vim-11H6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3
Immunofluorescence of monoclonal antibody to VIM on HeLa cell. [antibody concentration 40 ug/ml]
VIM monoclonal antibody (M01), clone 3E9 Western Blot analysis of VIM expression in HeLa ( Cat # L013V1 ).
Intracellular Flow Cytometry analysis of Vimentin expression in LEP-19 Human fibroblast cell line using PE conjugated anti-Human Vimentin antibody (VI-RE/1). Overlay with Isotype Mouse IgG1 control (PPV-06).
Immunohistochemistry analysis of Vimentin antibody (Cat.-No AP06478PU-N) in paraffin-embedded human colon carcinoma tissue.
Western blot (WB) analysis of Vimentin antibody (Cat.-No.: AP06478PU-N)
Western blot analysis of VIM ( arrow ) using rabbit VIM polyclonal antibody ( Cat # PAB2587 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the VIM gene ( Lane 2 ) ( Origene Technologies ) .
Western Blot analysis of VIM expression in transfected 293T cell line (H00007431-T02) by VIM MaxPab polyclonal antibody.Lane 1: VIM transfected lysate(53.70 KDa).Lane 2: Non-transfected lysate.
VIM MaxPab rabbit polyclonal antibody. Western Blot analysis of VIM expression in Hela NE.
Human COS7; submitted by:Carl LundinStanford UniversityThe Vimentin antibody ARP48225_P050 works well for visualization of the vimentin cytoskeleton (see attached images). We used PFA-fixed COS-7 cells and an antibody dilution of 1:400.; VIM antibody - N-terminal region (ARP48225_P050) in Human COS7 cells using Immunohistochemistry
Human brain stem; . Sample Type :. Human brain stem cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. VIM: Red DAPI:Blue. Gene Name :. VIM. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; VIM antibody - N-terminal region (ARP48225_P050) in Human brain stem cells using Immunohistochemistry
Human brain; WB Suggested Anti-VIM Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; VIM antibody - N-terminal region (ARP48225_P050) in Human brain cells using Western Blot
Human NT-2; Immunohistochemistry with NT-2 cell line tissue; VIM antibody - C-terminal region (ARP48226_P050) in Human NT-2 cells using Immunohistochemistry
Human NT-2; Immunohistochemistry with NT-2 cell line tissue; VIM antibody - C-terminal region (ARP48226_P050) in Human NT-2 cells using Immunohistochemistry
Human MCF-7; VIM antibody - C-terminal region (ARP48226_P050) validated by WB using MCF-7 whole cell lysates at 1.0ug/ml.; VIM antibody - C-terminal region (ARP48226_P050) in Human MCF-7 cells using Western Blot
Human NT-2; VIM antibody - C-terminal region (ARP48226_P050) validated by WB using 1. Human NT-2 cells (60ug) at 2ug/ml.; VIM antibody - C-terminal region (ARP48226_P050) in Human NT-2 cells using Western Blot
Hum. Fetal Lung; Host: Rabbit . Target Name: VIM . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; VIM antibody - C-terminal region (ARP48226_P050) in Hum. Fetal Lung cells using Western Blot
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of Vimentin (arrow) using Vimentin Antibody (Center) (AP12371PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
Human melanoma stained with anti-Vimentin Antibody Cat.-No AM11201PU (Clone SP20).
Western Blot analysis of HeLa cell lysate with anti-Vimentin Antibody Cat.-No AM11201PU (Clone SP20).
Flow Cytometric analysis of Rabbit anti- Vimentin Antibody Cat.-No AM11201PU (Clone SP20) in HeLa (green) compare to negative Control of Rabbit IgG (blue).
Western blot of crude extract of Hek293 cells stained with VIM polyclonal antibody ( Cat # PAB12100 ), showing a single strong clean band at ~50kDa.
Western blot analysis of VIM expression in mouse small intestine tissue lysate.
Immunofluorescence staining of NIH/3T3 ( mouse embryonal fibroblast cell line ) with VIM monoclonal antibody, clone VI-01 ( Cat # MAB3880 ). Nuclei are stained with DAPI ( blue ).
Immunofluorescence staining of RBL ( rat basophilic cell line ) with VIM monoclonal antibody, clone VI-01 ( Cat # MAB3880 ) conjugated with Dyomics 547. Nuclei are stained with DAPI ( blue ).
Western Blotting analysis of VIM in whole cell lysate of LEP-19 ( human fibroblast cell line ) ( 1, 3 ) and NIH/3T3 ( mouse embryonal fibroblast cell line ) ( 2, 4 ).Lane 1, 2 : Immunostaining with VIM monoclonal antibody, clone VI-01 ( Cat # MAB3880 ).Lane 3, 4 : Immunostaining with VIM monoclonal antibody, clone VI-RE/1.
Immunoprecipitation of VIM from HeLa cell lysate and detection by VIM monoclonal antibody, clone VI-01 ( Cat # MAB3880 ).IgM heavy chain ( 76-92 kDa ) and IgM light chain ( 25-30 kDa ) indicated.MW of VIM is 57 kDa.Lr = lysate ( reducing conditions ). Lnr = lysate ( non-reducing conditions ). IPr = immunoprecipitate ( reducing conditions ). IPnr = immunoprecipitate ( non-reducing conditions ).
Intracellular flow cytometry analysis of VIM expression in LEP-19 ( human fibroblast cell line ) using VIM monoclonal antibody, clone VI-RE/1 ( Cat # MAB3881 ) - PE.Overlay with Isotype mouse IgG1 control.
Intracellular flow cytometry analysis of VIM expression in LEP-19 ( human fibroblast cell line ) using VIM monoclonal antibody, clone VI-RE/1 (PE) ( Cat # MAB4527 ).Overlay with Isotype mouse IgG1 control.
Immunofluorescence staining of NIH/3T3 ( mouse embryonal fibroblast cell line ) with VIM monoclonal antibody, clone VI-01 (Dyomics 547) ( Cat # MAB5029 ). Nuclei are stained with DAPI ( blue ).
Immunofluorescence staining of RBL ( rat basophilic cell line ) with VIM monoclonal antibody, clone VI-01 (Dyomics 547) ( Cat # MAB5029 ). Nuclei are stained with DAPI ( blue ).
Immunofluorescence of purified MaxPab antibody to VIM on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of VIM expression in transfected 293T cell line (H00007431-T01) by VIM MaxPab polyclonal antibody.Lane 1: VIM transfected lysate(51.26 KDa).Lane 2: Non-transfected lysate.
1) phosphorylase B (a) BSA (b) ovalbumin (c) carbonic anhydrase (d) 2) vimentin
Immunohistochemistry analyzes of Vimentin antibody (Cat.-No.: AP20321PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot analyzes of Vimentin antibody (Cat.-No.: AP20321PU-N) in extracts from A549 cells.
Western blot analysis of Vimentin antibody (AP20372PU-N) in extracts from 293 cells.
In the Left panel (a tissue section through an e18 Mouse cerebellum), Vimentin (brown staining) can be seen in immature astrocytes of the white matter, as well as in vascular endothelial cells. In the Right panel (cultured dissociated cells from an e13 Mouse brain), vimentin (green staining) can be seen forming the cytoskeleton of immature astrocyte progenitor cells. DAPI (blue staining) allows visualization of nuclei.
Formalin-Fixed, Paraffin-Embedded Human solid fibrous tumor stained with anti-Vimentin antibody using peroxidase conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin antibody Cat.-No AM06159SU-N with DAB staining.
Western blot analysis using Vimentin antibody Cat.-No AM06159SU-N against truncated Vimentin recombinant protein.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin antibody Cat.-No AM06304SU-N with DAB staining.
Western blot analysis using Vimentin antibody Cat.-No AM06304SU-N against Hela (Lane 1), COS (Lane 2), HEK293 (Lane 3) and U20S (Lane 4) cell lysate.
Immunocytochemical detection of phosphorylated Vimentin (Ser55) on formaldehyde fixed U251 cells with AM26448AF-N.
Western blot analysis of phosphorylated Vimentin (Ser55) in U251 cells, M phase (1) and interphase (2) using AM26448AF-N.
Immunocytochemical detection of phosphorylated vimentin (Ser71) on 4% PFA fixed U251 cells with AM26458AF-N.
Western blot analysis of phosphorylated vimentin (Ser71) expression in U251 using AM26458AF-N.
Immunocytochemical detection of phosphorylated vimentin (Ser38) on 4% PFA fixed U251 cells with AM26459AF-N.
Western blot analysis of phosphorylated vimentin (Ser38) expression in U251 using AM26459AF-N.
Immunocytochemical detection of phosphorylated vimentin (Ser82) on 4% PFA fixed U251 cells with AM26460AF-N.
Western blot analysis of phosphorylated vimentin (Ser82) expression in U251 using AM26460AF-N.
Immunocytochemical detection of phosphorylated vimentin (Ser6) on 4% PFA fixed U251 cells with AM26461AF-N.
Western blot analysis of phosphorylated vimentin (Ser6) expression in U251 using AM26461AF-N.
Immunocytochemical detection of phosphorylated vimentin (Ser33) on 4% PFA fixed U251 cells with AM26464AF.
Western blot analysis of phosphorylated vimentin (Ser33) expression in U251 using AM26464AF.
Western blot analysis of Vimentin phosphorylation in U251 using AM26472AF-N.
Immunocytochemical detection of phosphorylated vimentin (Ser50) on 4% PFA fixed U251 cells with AM26472AF-N.
Intracellular flow cytometry analysis of Vimentin expression in LEP-19 human fibroblast cell line using anti-human Vimentin (VI-RE/1) PE.
Overlay with Isotype mouse IgG1 control.
Formalin-paraffin human melanoma stained with Vimentin Antibody Cat.-No AM32837PU (Clone VM452).
Mixed neuron/glial cultures stained with anti-Vimentin (green) and Rabbit anti-GFAP antibody (Cat.-No AP08673SU-N) (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.
Western blot of HeLa cells showing specific immunolabeling of the ~50k Vimentin protein.
Immunohistochemistry on paraffin section of human colon.
Immunofluorescence staining of the developing neural tube in a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining.
Immunofluorescence staining of a 1 month old zebrafish embryo.
Frozen Section of Swine colon immunostained with RV202-FITC Vimentin Antibody Cat.-No BM6008F (1/200)
Immunohistochemistry using Vimentin Antibody Cat.-No BM6008P on Frozen Section of Swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells.
Frozen Section of Swine colon immunostained with RV202-HRP Vimentin Antibody Cat.-No BM6008HRP (1/200).
Immunohistochemistry on paraffin section of human colon.
Immunofluorescence staining of a 1 month old zebrafish embryo.
Immunofluorescence staining of the developing neural tube in a 2 days old zebrafish embryo. Left panel: DAPIstaining of cell nuclei, providing an overview of the tissue section used for immunostaining.
Immunohistochemistry using Vimentin Antibody Cat.-No BM6008P on Frozen Section of Swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells.
Frozen Section of Swine colon immunostained with RV202-FITC Vimentin Antibody Cat.-No BM6008F (1/200)
Frozen Section of Swine colon immunostained with RV202-HRP Vimentin Antibody Cat.-No BM6008HRP (1/200).
Formalin-paraffin human melanoma stained with Vimentin Antibody Cat.-No AM50186PU (Clone SPM576).
Western blot analysis of extracts from A549 cells treated with Nocodazole using Phospho-Vimentin (Ser56) antibody AP55892PU-N.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of Vimentin (pS56) expression in MCF7 UV-treated (A); A10 (B); KNRK (C); NIH3T3 UV-treated (D); H9C2 UV-treated (E) whole cell lysates.
Immunohistochemical analysis of Vimentin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Vimentin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Vimentin expression in MCF7 (A); MDAMB435 (B); KNRK (C); HeLa (D); Raw264.7 (E); PC12 (F) whole cell lysates.
VIM Luminex Elisa with 13B1 Capture (TA600389) and 1A9 Detection (TA700389) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM ELISA with 5D7 Capture (TA600390) and 7B10 Detection (TA700390) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM Luminex Elisa with 5D7 Capture (TA600390) and 7B10 Detection (TA700390) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM ELISA with 1A9 Capture (TA600391) and 5C10 Detection (TA700391) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM Luminex Elisa with 1A9 Capture (TA600391) and 5C10 Detection (TA700391) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM ELISA with 5C10 Capture (TA600392) and 7B10 Detection (TA700390) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM Luminex Elisa with 5C10 Capture (TA600392) and 7B10 Detection (TA700390) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM Luminex Elisa with 7A9 Capture (TA600393) and 5C10 Detection (TA700391) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM Luminex Elisa with 13B1 Capture (TA600389) and 1A9 Detection (TA700389) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM ELISA with 5C10 Capture (TA600392) and 7B10 Detection (TA700390) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM ELISA with 5D7 Capture (TA600390) and 7B10 Detection (TA700390) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM Luminex Elisa with 5C10 Capture (TA600392) and 7B10 Detection (TA700390) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM Luminex Elisa with 5D7 Capture (TA600390) and 7B10 Detection (TA700390) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM ELISA with 1A9 Capture (TA600391) and 5C10 Detection (TA700391) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM Luminex Elisa with 1A9 Capture (TA600391) and 5C10 Detection (TA700391) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
VIM Luminex Elisa with 7A9 Capture (TA600393) and 5C10 Detection (TA700391) Antibodies. Substrate used: Recombinant Human VIM (TP301546)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801250)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801297)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801319)
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801319)
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801319)
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801319)
Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801319)
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801319)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Immunofluorescent analysis of Vimentin staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Vimentin expression in HepG2 (A); A549 (B) whole cell lysates.
Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein.
Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.
Western blot analysis of extracts from HepG2 cells, treated with Adriamycin (0.5uM, 5hours), using Vimentin antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells (Lane 2), using Vimentin Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Vimentin antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of Vimentin-S82 (Cat. #TA324728) in Hela cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab.
Western blot analysis of VIM(arrow) using rabbit polyclonal Vimentin Antibody (S82) (Cat.#TA324728). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82) (Cat.#TA324728), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF image of SY5Y cells stained with Vimentin (S82) antibody. SY5Y cells were incubated with TA324728 Vimentin (S82) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).
Western blot analysis of Vimentin antibody (C-term) (Cat.#TA324729) in NCI-H460 cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab .
Vimentin Antibody (C-term) (Cat. #TA324729)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
IF image of SY5Y cells stained with Vimentin (C-term) antibody. SY5Y cells were incubated with TA324729 Vimentin (C-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).
Vimentin Antibody (C-term) (Cat. #TA324729) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of Vimentin expression in Nocodazole treated A549 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of Vimentin expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis on 293 cell lysate using Vimentin Antibody
Immunohistochemistry staining of Paraffin Tonsil tissue by Vimentin antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic)
Western Blot: Vimentin Antibody [TA336623] - Western blot of crude extract of human embryonic kidney Hek293 cells stained with TA336623, showing a single strong clean band at approx. 50kDa.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [TA336623] - View of mixed neuron/glial cultures stained with TA336623 (green) and rabbit antibody to GFAP antibody NB300-141(red). Vimentin is expressed alone in fibroblastic and endothelial cell
Western Blot: Vimentin Antibody (2D1) [TA336856] - Western blot of crude extract HeLa. Lane 18 was probed with TA336856 antibody. Note the strong clean band at the expected molecular weight of 50kDa. Lane 17 was probed with a monoclonal antibody to Viment
Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2D1) [TA336856] - View of mixed neuron/glial cultures stained with TA336856 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial cel
Western Blot: Vimentin Antibody (2A52) [TA336857] - Western blot of crude extract of the human carcinoma HeLa cell line showing strong clean bands at the expected molecular weight of 50kDa. Lane 18 was probed with TA336857 antibody, whereas, Lane 17 was p
Immunocytochemistry/Immunofluorescence: Vimentin Antibody (2A52) [TA336857] - View of mixed neuron/glial cultures stained with NBP1-92687 (green) and the GFAP rabbit polyclonal (NB300-141, red). Vimentin is expressed alone in fibroblastic and endothelial
Western Blot: Vimentin Antibody [TA337118] - Blot of crude extract of HeLa cells, showing a single strong clean band at 55 kDa.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [TA337118] - View of HeLa cells stained with TA337118 (red) and counterstained with monoclonal antibody to Lamin A + C, NBP2-25151 (green). DNA is blue. TA337118 reveals strong cytoplasmic intermed
TA348941 (2 ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of Vimentin expression in Hela (A); mouse brain (B); rat brain (C) whole cell lysates.
Immunohistochemical analysis of Vimentin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Vimentin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Vimentin expression in Hela (A); mouse brain (B); rat brain (C) whole cell lysates.
Immunohistochemical analysis of Vimentin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western Blot analysis of HeLa cell lysate with vimentin antibody
Human Melanoma stained with anti-Vimentin antibody.
Flow cytometric analysis of rabbit anti-Vimentin (SP20) antibody in HeLa (green) compare to negative control of rabbit IgG (blue).
Human tosil stained with anti-vimentin antibody.
Human Colon Adencarcinoma stained with anti-Vimentin antibody.
Human Prostate Adenocarcinoma stained with anti-Vimentin antibody.
Human Hepatocellular Carcinoma stained with anti-Vimentin antibody.
Human Lung Squamous Cell Carcinoma stained with anti-Vimentin antibody.
Human Renal Cell Carcinoma stained with anti-Vimentin antibody.
Human Breast Ductal Cell Carcinoma stained with anti-Vimentin antibody.
Human Bladder Transitional Cell Carcinoma stained with anti-Vimentin antibody.
Human Ovary Adenocarcinoma stained with anti-Vimentin antibody.
Human Stomach Adenocarcinoma stained with anti-Vimentin antibody.
IF stainning for Vimentin 3 full length positive (V9) was positive and the IF for Vim3 was negative in the papilary RCC component (the top panel), indicating the malignant tumour subtype. Whereas the bottom panel is an oncocytoma and Vim3 positive.
- Quick-Filters:
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA309274 | Vimentin antibody |
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| Chicken Anti-Vimentin Antibody | |||||||
 |
Chicken | Ig | Ig Fraction | Hu, Mam, Ms, Rt | C, ICC/IF, WB |
0.1 ml /
€355.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
Published recently! |
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| SM3128P | Vimentin antibody |
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 |
Mouse | IgM | VI-01 | Purified | All Species | ICC/IF, WB |
0.1 mg /
€240.00
|
| OriGene Technologies GmbH | |||||||
| +3 additional images |
Published recently! Highly validated antibody! |
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| SM3129P | Vimentin antibody |
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 |
Mouse | IgG1 | VI-RE/1 | Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€240.00
|
| OriGene Technologies GmbH | |||||||
Published recently! |
|||||||
| SM3166P | Vimentin antibody |
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 |
Mouse | IgM | VI-10 | Purified | Chk, Hu, Por, Rt | ICC/IF, IP, P, WB |
0.1 mg /
€240.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
Published recently! |
||||||
| UM800054 | Vimentin antibody |
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| Vimentin (VIM) mouse monoclonal antibody,clone UMAB159 | |||||||
 |
Mouse | IgG1 | UMAB159 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| UM800055 | Vimentin antibody |
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| Vimentin (VIM) mouse monoclonal antibody,clone UMAB160 | |||||||
 |
Mouse | IgG1 | UMAB160 | Purified | Hu | P |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
||||||
| UM870054 | Vimentin antibody |
||||||
| Vimentin (VIM) mouse monoclonal antibody,clone UMAB159 | |||||||
|
Mouse | IgG1 | UMAB159 | Purified | Hu | ICC/IF, P, WB |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
||||||
| UM870055 | Vimentin antibody |
||||||
| Vimentin (VIM) mouse monoclonal antibody,clone UMAB160 | |||||||
|
Mouse | IgG1 | UMAB160 | Purified | Hu | P |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
||||||
| TA324728 | Vimentin antibody |
||||||
| Rabbit polyclonal Vimentin Antibody (S82) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
||||||
| TA700390 | Vimentin antibody |
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| Luminex and ELISA validated biotinylated VIM mouse monoclonal antibody (clone 7B10), clone OTI7B10 (formerly 7B10) | |||||||
 |
Mouse | IgG1 | OTI7B10 | Purified | Hu | E, LMNX |
50 µg /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
||||||
| TA801250 | Vimentin antibody |
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| VIM mouse monoclonal antibody, clone OTI5D7 (formerly 5D7) | |||||||
 |
Mouse | IgG2a | OTI5D7 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +13 additional images |
Highly validated antibody!
|
||||||
| TA801297 | Vimentin antibody |
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| VIM mouse monoclonal antibody, clone OTI1A9 (formerly 1A9) | |||||||
 |
Mouse | IgG1 | OTI1A9 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +16 additional images |
Highly validated antibody!
|
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| TA801319 | Vimentin antibody |
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| VIM mouse monoclonal antibody, clone OTI5C10 (formerly 5C10) | |||||||
 |
Mouse | IgG1 | OTI5C10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| AM32837PU-S | Vimentin antibody |
||||||
 |
Mouse | IgG1 | VM452 | Purified | Bov, Can, Chk, Fe, Gt, Hu, Por | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM32837PU-T | Vimentin antibody |
||||||
|
Mouse | IgG1 | VM452 | Purified | Bov, Can, Chk, Fe, Gt, Hu, Por | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM50186PU-S | Vimentin antibody |
||||||
 |
Mouse | IgG1 | SPM576 | Purified | Bov, Can, Chk, Fe, Gt, Hu, Por | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| AM50186PU-T | Vimentin antibody |
||||||
|
Mouse | IgG1 | SPM576 | Purified | Bov, Can, Chk, Fe, Gt, Hu, Por | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
|||||||
| TA312104 | Vimentin antibody |
||||||
| Rabbit polyclonal anti-Vimentin antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA324729 | Vimentin (C-term) antibody |
||||||
| Rabbit polyclonal Vimentin Antibody (C-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
|||||||
| TA325972 | Vimentin antibody |
||||||
| Rabbit Polyclonal Vimentin Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
