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Vinculin (VCL) antibody

Principal name

Vinculin (VCL) antibody

Alternative names for Vinculin (VCL) antibody

Metavinculin

SwissProt ID

O46037 (Drome), P12003 (Chick), P18206 (Human), P26234 (Pig), P85972 (Rat), Q04615 (Xenla), Q64727 (Mouse)

Gene ID

7414 (VCL), 22330 (Vcl), 305679

Ncbi ID

NP_003364.1, 9606, NP_003364, NM_003373, AAH39174.1, NM_009502, NM_001107248

Available reactivities

Chk (Chicken), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Por (Porcine), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of Vinculin (VCL) antibody

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-termil head, a short, flexible proline-rich region and a carboxy-termil tail. In the ictive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration. Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-a and Tyr100 and Tyr1065 by Src kises weakens the head-tail interaction. This change in vinculin allows the binding of a number of other proteins, including talin, a-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformatiol change from the ictive to active state. Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth. This is supported by a recently demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma.

General readings

1. Bakolitsa, C., Cohen, D. M., Bankston, L. A., Bobkov, A. A., Cadwell, G. W., Jennings, L., Critchley,
D. R., Craig, S. W., Liddington, R. C.Structural basis for vinculin activation at sites of cell
adhesion.Nature 430: 583-586, 2004.
2. Mulligan, L. M., Gardner, E., Telenius, H., Ponder, B. A. J.Complementary physical and genetic
techniques map the vinculin (VCL) gene on chromosome 10q.Genomics 13: 1347-1349, 1992.
3. Vasile, V. C., Edwards, W. D., Ommen, S. R., Ackerman, M. J.Obstructive hypertrophic
cardiomyopathy is associated with reduced expression of vinculin in the intercalated disc.Biochem.
Biophys. Res. Commun. 349: 709-715, 2006.

SM1375 Vinculin antibody Staining of Paraffin Embedded Formalin Fixed Human appendix.

SM1375

Immunofluorescence of monoclonal antibody to VCL on HeLa cell. [antibody concentration 10 ug/ml]

H00007414-M01

VCL monoclonal antibody (M01), clone 2C6-1B5 Western Blot analysis of VCL expression in Hela ( Cat # L013V1 ).

H00007414-M01

Immunohistochemistry (IHC) analyzes of Vinculin antibody (Cat.-No.: AP06605PU-N) in paraffin-embedded Human breast carcinoma tissue.

AP06605PU-N

Western blot (WB) analysis of Vinculin antibody (Cat.-No.: AP06605PU-N) in extracts from Hela cells.

AP06605PU-N

Detection limit for recombinant GST tagged VCL is approximately 0.1ng/ml as a capture antibody.

H00007414-M02

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with VINC antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

AP14070PU-N

(LEFT) Western blot analysis of anti-VINC Antibody (N-term) in K562 cell line lysates (35ug/lane). VINC (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-VINC Antibody (N-term) in mouse lung tissue lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.

AP14070PU-N

(LEFT) Western blot analysis of anti-VINC Antibody (C-term) in Ramos cell line lysates (35ug/lane). VINC (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of anti-VINC Antibody (C-term) in mouse lung tissue lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.

AP14071PU-N

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HepG2 cells, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Vinculin (Ab-821) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using Vinculin (Ab-821) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, treated with forskolin 40nM 30', using Vinculin (Ab-821) Antibody. The lane on the right is treated with the synthesized peptide.

Human heart stained with anti-Vinculin antibody

AP23808PU Vinculin antibody staining of Human Pancreas.

Human HepG2; WB Suggested Anti-VCL Antibody. . Titration: 1.0 ug/ml. . Positive Control: HepG2 Whole Cell; VCL antibody - C-terminal region (ARP61099_P050) in Human HepG2 cells using Western Blot

ARP61099_P050

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP31334PU-N

Human Kidney, Tubules: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP31334PU-N

Immunofluorescence analysis of A549 cells, using Vinculin Antibody. The picture on the right is treated with the synthesized peptide.

AP31334PU-N

Western blot analysis of extracts from HeLa cells, treated with forskolin 40nM 30 minutes, using Vinculin Antibody. The lane on the right is treated with the synthesized peptide.

AP31334PU-N

Western blot analysis of extracts from HeLa cells treated with Forskolin using Phospho-Vinculin (Tyr822) antibody AP55923PU-N.The lane on the right is treated with the antigen-specific peptide.

Immunohistochemical analysis of Vinculin staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Vinculin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of Vinculin expression in MCF7 (A); HepG2 (B) whole cell lysates.

TP304576

Predicted band size: 123 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/1000-4000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1200 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute)

TA322857

Predicted cell location: Cytoplasm. Positive control: Human liver and lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using VCL antibody at dilution 1/13, on the right is immunohistochemistry of paraffin-embedded Human lung cancer tissue using VCL antibody at dilution 1/13. (Original magnification:x200)

TA322857

Western blot analysis of Vinculin in PC-3 cell lysate with Vinculin antibody at (A) 1 and (B) 2 ug/ml.

TA326660

Immunohistochemistry of Vinculin in rat small intestine tissue with Vinculin antibody at 5 ug/mL.

TA326660

Immunofluorescence of Vinculin in rat small intestine tissue with Vinculin antibody at 20 ug/mL.

TA326660

Western blot analysis of extracts of various cell lines, using VCL antibody.

TA327166

Immunohistochemistry of paraffin-embedded rat brain using VCL antibody at dilution of 1:200 (400x lens).

TA327166

WB Suggested Anti-VCL Antibody; Titration: 1.0 ug/ml; Positive Control: HepG2 Whole Cell

TA343106

Western blot analysis of vinculin expression in HepG2

TA347873

Western blot analysis of Vinculin in 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and rat heart, mouse lung, rat lung, mouse spleen, rat spleen, rabbit spleen, mouse brain, rabbit brain and chicken spleen tissue lysate with Vinculin ant

TA349007

Western blot analysis of Vinculin in A431, Daudi, Jurkat, Raji, and THP-1 cell lysate with Vinculin antibody at 1 ug/mL.

TA349057

Western blot analysis of Vinculin expression in Hela (A); K562 (B); mouse brain (C); rat brain (D) whole cell lysates.

Immunohistochemical analysis of Vinculin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Human Heart stained with anti-Vinculin antibody.

TA354150

Human Heart stained with anti-SMA antibody.

TA354150

Human Cerebellum stained with anti-Vinculin antibody.

TA354150

Human Skeletal Muscle stained with anti-Vinculin antibody.

TA354150

Human Prostate stained with anti-Vinculin antibody.

TA354150

Human Colon stained with anti-Vinculin antibody.

TA354150

Human Small Intestine stained with anti-Vinculin antibody.

TA354150

Human Stomach stained with anti-Vinculin antibody.

TA354150

Human Bladder stained with anti-Vinculin antibody.

TA354150

Human Breast stained with anti-Vinculin antibody.

TA354150

Human Breast Adenocarcinoma stained with anti-Vinculin antibody.

TA354150

Human Pancreas stained with anti-Vinculin antibody.

TA354149

Western blot analysis of Vinculin phosphorylation expression in forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325968

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP31334PU-N	Vinculin (VCL) antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, ICC/IF, P, WB	50 µl / €425.00
						OriGene Technologies GmbH
+3 additional images	Highly validated antibody!

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Vinculin (VCL) antibody

Principal name

Alternative names for Vinculin (VCL) antibody

SwissProt ID

Gene ID

Ncbi ID

Available reactivities

Available hosts

Available applications

Background of Vinculin (VCL) antibody

General readings

Primary Antibodies

Vinculin (VCL) antibody

Service