VPS28 antibody
Principal name
VPS28 antibody
Alternative names for VPS28 antibody
Vacuolar protein sorting-associated protein 28 homolog, ESCRT-I complex subunit VPS28
Gene ID
51160 (VPS28)
Ncbi ID
9606, NP_057292, ADZ15838.1, NP_057292.1, NM_183057, NP_898880, NM_016208
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of VPS28 antibody
- Kit Component:
- - KN200025G1, VPS28 gRNA vector 1 in pCas-Guide vector
- KN200025G2, VPS28 gRNA vector 2 in pCas-Guide vector
- KN200025D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Bishop N, Woodman P.
TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes.
J Biol Chem. 2001 Apr 13;276(15):11735-42.
PMID: 11134028
VPS28 polyclonal antibody ( Cat # PAB6584 ) ( 10 µg/ml ) staining of paraffin embedded human testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
VPS28 polyclonal antibody ( Cat # PAB6584 ) staining ( 1 µg/ml ) of human testis lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Recombinant human VPS28, 1-221 aa: 15% SDS-PAGE (3 µg)
mouse brain extract; WB Suggested Anti-VPS28 Antibody . . Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extract. Primary Antibody Dilution : 1:500. Secondary Antibody : Anti rabbit-HRP . Secondry Antibody Dilution : 1:5,000. Submitted by: Scott Wilson, University of Alabama at Birmingham; VPS28 antibody - N-terminal region (ARP56850_P050) in mouse brain extract cells using Western Blot
Human Liver; WB Suggested Anti-VPS28 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Liver; VPS28 antibody - N-terminal region (ARP56850_P050) in Human Liver cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VPS28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505691)
Anti-VPS28 mouse monoclonal antibody (TA505691) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VPS28(RC200025).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-VPS28 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28 (RC200025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VPS28.
TA302924 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302924 (10µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
WB Suggested Anti-VPS28 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Liver
WB Suggested Anti-VPS28 Antibody ; Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extract; Primary Antibody Dilution : 1:500; Secondary Antibody : Anti rabbit-HRP; Secondry Antibody Dilution : 1:5,000; Submitted by: Scott Wils
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA505691 | VPS28 antibody |
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| VPS28 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8) | |||||||
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Mouse | IgG1 | OTI1A8 | Purified | Can, Hu, Mky, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Top Selling product! Highly validated antibody!
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| TA302924 | VPS28 (C-term) antibody |
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| Goat Polyclonal Antibody against VPS28 | |||||||
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Goat | Aff - Purified | Can, Hu, Ms, Por, Rt | P, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA350605 | VPS28 antibody |
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| Rabbit Polyclonal Anti-VPS28 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300025 | VPS28 (transcript variant 1) |
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| Recombinant protein of human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP308726 | VPS28 (transcript variant 2) |
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| Recombinant protein of human vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY405220 | VPS28 overexpression lysate |
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |