VPS53 antibody
Principal name
VPS53 antibody
Alternative names for VPS53 antibody
PP13624, Vacuolar protein sorting-associated protein 53 homolog
Ncbi ID
NP_001121631, NP_001121631.1, NP_080940.2, BC034371, NM_026664, NM_018289, NM_001128159
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of VPS53 antibody
- Kit Component:
- - KN319266G1, Vps53 gRNA vector 1 in pCas-Guide vector
- KN319266G2, Vps53 gRNA vector 2 in pCas-Guide vector
- KN319266D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of VPS53 expression in transfected 293T cell line by VPS53 MaxPab polyclonal antibody.Lane 1: VPS53 transfected lysate(73.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry of VPS53 in 293 cells with VPS53 polyclonal antibody ( Cat # PAB13369 ) at 5 µg/ml.
Western blot analysis of VPS53 in 293 cell lysate with VPS53 polyclonal antibody ( Cat # PAB13369 ) at ( A ) 0.5 and ( B ) 1 µg/ml.
Western blot analysis of VPS53 in 293 cell lysate with VPS53 polyclonal antibody ( Cat # PAB13370 ) at ( A ) 0.5 and ( B ) 1 µg/ml.
(LEFT) Western blot analysis of VPS53 Antibody (N-term) (AP18239PU-N) in MCF-7 cell line lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of VPS53 Antibody (N-term) (AP18239PU-N) in mouse brain tissue lysates (35ug/lane). VPS53 (arrow) was detected using the purified Pab.
Human Muscle; WB Suggested Anti-VPS53 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; VPS53 antibody - middle region (ARP57192_P050) in Human Muscle cells using Western Blot
Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry of VPS53 in 293 cells with VPS53 antibody at 5 ug/mL.
Immunofluorescence of VPS53 in 293 cells with VPS53 antibody at 2.5 ug/mL.
Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 ug/ml.
WB Suggested Anti-VPS53 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306570 | VPS53 (C-term) antibody |
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| Rabbit Polyclonal VPS53 Antibody | |||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€415.00
|
||
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA306571 | VPS53 (Center) antibody |
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| Rabbit Polyclonal VPS53 Antibody | |||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€415.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350607 | VPS53 antibody |
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| Rabbit Polyclonal Anti-VPS53 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP18239PU-N | VPS53 (N-term) antibody |
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 |
Rabbit | Hu, Ms | WB |
0.4 ml /
€415.00
|
|||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP326200 | VPS53 (transcript variant 1) |
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| Purified recombinant protein of Homo sapiens vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209806L2V | Lenti ORF particles, Vps53 (GFP-tagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR218740L2V | Lenti ORF particles, Vps53 (GFP-tagged) - Mouse vacuolar protein sorting 53 (yeast) (Vps53), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,660.00
|
|
| OriGene Technologies, Inc. | ||
| MR209806L1V | Lenti ORF particles, Vps53 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 53 (yeast) (cDNA clone MGC:19253 IMAGE:3964008), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR218740L1V | Lenti ORF particles, Vps53 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 53 (yeast) (Vps53), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,490.00
|
|
| OriGene Technologies, Inc. | ||