VPS72 antibody
Principal name
VPS72 antibody
Alternative names for VPS72 antibody
TCFL1, YL1, Vacuolar protein sorting-associated protein 72 homolog
Ncbi ID
NP_005988, 9606, NP_005988.1, NM_009336, NM_005997, NP_033362.2
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of VPS72 antibody
- Kit Component:
- - KN319268G1, Vps72 gRNA vector 1 in pCas-Guide vector
- KN319268G2, Vps72 gRNA vector 2 in pCas-Guide vector
- KN319268D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Horikawa,I., et al., (1995) Oshimura,M. Biochem. Biophys. Res. Commun. 208 (3), 999-1007
Human Lung; WB Suggested Anti-TCFL1 Antibody Titration: 0.06ug/ml. Positive Control: Human Lung; TCFL1 antibody - middle region (ARP34072_P050) in Human Lung cells using Western Blot
Immunofluorescence of monoclonal antibody to VPS72 on HeLa cell . [antibody concentration 10 ug/ml]
Detection limit for recombinant GST tagged VPS72 is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of VPS72 expression in transfected 293T cell line (H00006944-T01) by VPS72 MaxPab polyclonal antibody.Lane 1: VPS72 transfected lysate(40.60 KDa).Lane 2: Non-transfected lysate.
Human ACHN; WB Suggested Anti-VPS72 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: ACHN cell lysate; VPS72 antibody - N-terminal region (ARP33348_P050) in Human ACHN cells using Western Blot
Mouse Heart; WB Suggested Anti-Vps72 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Vps72 antibody - N-terminal region (ARP33349_P050) in Mouse Heart cells using Western Blot
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from RAW264.7/NIH-3T3 cells, using VPS72 Antibody Cat.-No AP32269PU-N. The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from RAW264.7 cells and NIH/3T3 cells, using VPS72 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using VPS72 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-VPS72 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:2500; Positive Control: ACHN cell lysate
WB Suggested Anti-TCFL1 Antibody Titration: 0.06 ug/ml; Positive Control: Human Lung
Western blot analysis on RAW264.7 cell lysate using VPS72 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA314603 | VPS72 antibody |
||||||
| Rabbit polyclonal anti-VPS72 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| TA347633 | VPS72 antibody |
||||||
| Rabbit Polyclonal Anti-VPS72 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP32269PU-N | VPS72 antibody |
||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, P, WB |
50 µl /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301273 | VPS72 |
||||
| Recombinant protein of human vacuolar protein sorting 72 homolog (S. cerevisiae) (VPS72) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY416942 | VPS72 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205675L2V | Lenti ORF particles, Vps72 (GFP-tagged) - Mouse vacuolar protein sorting 72 (yeast) (Vps72), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205675L1V | Lenti ORF particles, Vps72 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 72 (yeast) (Vps72), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||