VRK1 antibody
Principal name
VRK1 antibody
Alternative names for VRK1 antibody
Vaccinia-related kinase 1, Serine/threonine-protein kinase VRK1
Gene ID
7443 (VRK1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP), Paraffin Sections (P)
Background of VRK1 antibody
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Western Blot analysis of VRK1 expression in transfected 293T cell line by VRK1 monoclonal antibody (M02), clone 4F9.Lane 1: VRK1 transfected lysate(45.5 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of VRK1 over-expressed 293 cell line, cotransfected with VRK1 Validated Chimera RNAi ( Cat # H00007443-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK1 monoclonal antibody (M02) clone 4F9 (Cat # H00007443-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Detection limit for recombinant GST tagged VRK1 is approximately 0.1ng/ml as a capture antibody.
The VRK1 polyclonal antibody ( Cat # PAB2882 ) is used in Western blot to detect VRK1 in mouse lung tissue lysate ( Lane 1 ) and HL-60 cell lysate ( Lane 2 ) .
Immunofluorescence of purified MaxPab antibody to VRK1 on HeLa cell. [antibody concentration 10 ug/ml]
VRK1 MaxPab polyclonal antibody. Western Blot analysis of VRK1 expression in human pancreas.
Western Blot analysis of VRK1 expression in transfected 293T cell line (H00007443-T01) by VRK1 MaxPab polyclonal antibody.Lane 1: VRK1 transfected lysate(43.56 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of VRK1 expression in transfected 293T cell line (H00007443-T02) by VRK1 MaxPab polyclonal antibody.Lane 1: VRK1 transfected lysate(45.50 KDa).Lane 2: Non-transfected lysate.
VRK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of VRK1 expression in Hela NE.
The anti-VRK1 Pab is used in Western blot to detect VRK1 in mouse lung tissue lysate (Lane 1) and HL-60 cell lysate (Lane 2).
Western Blot: VRK1 Lysate [NBL1-17759] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for VRK1.
VRK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of VRK1 expression in Hela NE.
Immunoprecipitation of VRK1 transfected lysate using anti-VRK1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with VRK1 MaxPab mouse polyclonal antibody (B01) (H00007443-B01).
Immunohistochemical analysis of paraffin-embedded Liver, using VRK1(GTX107899) antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE GTX107899 diluted at 1:1000
Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-85822] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Immunofluorescence: VRK1 Antibody [NBP1-85822] - 251MG shows positivity in nucleus but not nucleoli.
Western Blot: VRK1 Antibody [NBP1-85822] Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1 | Lane 2: RT-4 | Lane 3: EFO-21 | Lane 4: U-138 MG | Lane 5: Liver | Lane 6: Tonsil
Immunohistochemistry-Paraffin: VRK1 Antibody [NBP1-89548] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
Immunofluorescence: VRK1 Antibody [NBP1-89548] - 431 shows positivity in nucleus & nucleoli.
Western Blot: VRK1 Antibody [NBP1-89548] Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Immunohistochemistry was performed on normal biopsies of deparaffinized human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and then probed without (left panel) or with (right panel) VRK1 antibody [1F6] at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and Streptavidin-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Western blot analysis of VRK1 in 25 ug of various cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with VRK1 antibody [1F6] at a dilution of 1:750 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a HRP-conjugated secondary antibody. Chemiluminescent detection was performed.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VRK1 (RC210764, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VRK1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VRK1 (RC210764, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VRK1.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA325175 | VRK1 antibody |
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| Mouse monoclonal VRK1 Antibody (Center)(Ascites) | |||||||
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Mouse | IgG1 | 1015CT2.1.1 | Ascites | Hu | WB |
0.1 ml /
€415.00
|
| OriGene Technologies, Inc. | |||||||
| TA805595 | VRK1 antibody |
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| VRK1 mouse monoclonal antibody, clone OTI2C9 (formerly 2C9) | |||||||
 |
Mouse | IgG1 | OTI2C9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA805791 | VRK1 antibody |
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| VRK1 mouse monoclonal antibody, clone OTI2E10 (formerly 2E10) | |||||||
 |
Mouse | IgG1 | OTI2E10 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AP14050PU-N | VRK1 (Center) antibody |
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Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760293 | VRK1 (full length, N-term HIS tag) |
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| Recombinant protein of human vaccinia related kinase 1 (VRK1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418719 | VRK1 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
