Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » WDHD1 antibody

WDHD1 antibody

(reset)

Principal name

WDHD1 antibody

Alternative names for WDHD1 antibody

AND1, AND-1, Acidic nucleoplasmic DNA-binding protein 1, WD repeat and HMG-box DNA-binding protein 1

SwissProt ID

O13046 (Xenla), O75717 (Human), P59328 (Mouse)

Gene ID

11169 (WDHD1), 218973 (Wdhd1)

Ncbi ID

8355, 9606, NP_001008397.1, NP_009017, NP_766186.2, NM_007086

Available reactivities

Xen (Xenopus), Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Eq (Equine), Por (Porcine), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of WDHD1 antibody

AND-1 (WDHD1) also known as WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1. AND-1 (WDHD1) is a nuclear and nucleolar protein that contains multiple WD40 repeats, and one HMG region. AND-1 (WDHD1) has a predicted molecular weight of 126 kD and can form homodimers. WDHD1 is expressed in the brain, epidermis, liver, and stomach. During mitosis, AND-1 (WDHD1) is transiently expressed in nucleoplasm. AND-1 (WDHD1) can bind to DNA with high affinity and may be involved in protein-protein interactions. The role of AND-1 (WDHD1) is thought to be chromatin assembly, transcription and DNA replication. AND-1 (WDHD1) has been reported to be phosphorylated on S1041. 
Structure: Contains multiple WD40 repeats, one HMG region. Predicted molecular weight 126 kD. Forms homodimers.
Distribution: Found in the nucleus and nucleolus. Expressed in brain, epidermis, liver, and stomach. During mitosis transiently expressed in nucleoplasm.
Function: Binds DNA with high affinity; may be involved in protein-protein interactions. Thought to function in chromatin assembly, transcription and DNA replication.
Modification: Phosphorylation (S1041).

General readings

Zhu,W., (2007) Genes Dev. 21 (18), 2288-2299

ARP38902_P050 - WDHD1
Human HeLa; WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate; WDHD1 antibody - C-terminal region (ARP38902_P050) in Human HeLa cells using Western Blot
ARP32844_P050 - WDHD1
Human HepG2; WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; WDHD1 antibody - middle region (ARP32844_P050) in Human HepG2 cells using Western Blot
C19553-1 - WDHD1
Western blot analysis of extracts from Jurkat/HepG2 cells, using WDHD1 Antibody. The lane on the right is treated with the synthesized peptide.
AM06023PU-T - WDHD1
Jurkat nuclear cell extracts (lane 1) and NIH3T3 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-AND-1 antibody (clone 20G10). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
AM06023PU-T - WDHD1
Formalin-fixed paraffin-embedded human kidney tissue was stained with 20G10 at 15 ug/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
CPA2535-100ul - WDHD1
Immunohistochemical analysis of WDHD1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA2535-100ul - WDHD1
Western blot analysis of WDHD1 expression in HeLa (A); HEK293T (B) whole cell lysates.
TP316745 - WDHD1
TA315156 - WDHD1
Western blot analysis of extracts from HepG2 cells and Jurkat cells, using WDHD1 antibody.The lane on the right is treated with the synthesized peptide.
TA331196 - WDHD1
WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate
TA343657 - WDHD1
WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WDHD1 antibody
  Rabbit polyclonal anti-WDHD1 antibody  
Western blot analysis of extracts from HepG2 cells and Jurkat cells, using WDHD1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

WDHD1 (C-term) antibody
  Mouse Monoclonal anti-AND-1 (WDHD1) Antibody  
Jurkat nuclear cell extracts (lane 1) and NIH3T3 nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-AND-1 antibody (clone 20G10). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG2a 20G10 Aff - Purified Hu, Ms P, WB
0.1 mg / €275.00
  OriGene Technologies, Inc.
+1 additional image

WDHD1 antibody
WDHD1 Rabbit Serum Hu C, E, WB
0.2 ml / €430.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WDHD1 (transcript variant 1)
  Recombinant protein of human WD repeat and HMG-box DNA binding protein 1 (WDHD1), transcript variant 1  
WDHD1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.
 

4 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (3)
  2. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (2)
  2. Monoclonal (1)
Target
  1. WDHD1 (4)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (1)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (2)
  3. Frozen sections (C) (1)
  4. Paraffin Sections (P) (1)
Presentation
  1. Aff - Purified (1)
  2. Purified (1)
  3. Serum (1)
Host
  1. Rabbit (2)
  2. Mouse (1)
Images
  1. 1 image (3)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (4)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn