WIF1 antibody
Principal name
WIF1 antibody
Alternative names for WIF1 antibody
WIF-1, UNQ191/PRO217, Wnt inhibitory factor 1
Ncbi ID
9606, NP_009122.2, NP_036045.1, NM_007191, AAH18037, NP_036045, NP_009122, BC004048
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of WIF1 antibody
WIF-1 (Wnt Inhibitory Factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity. In situ hybridization analysis in Xenopus laevis and zebrafish indicate that the message is highly expressed in presomitic mesoderm, the notochord, anterior regions of the brain, branchial arches, nasal placodes, and otic vescicles. WIF-1 inhibits secondary axis induction by Wnts and promotes secondary axis induction by Chordin in Xenopus embryos. In vitro, WIF-1 binds to Drosophila Wingless and Xenopus Wnt8 proteins. WIF-1 is implicated as an early event tumor suppressor in cancers of the prostate, breast, lung, and bladder, while in other cancer types, such as colon adenocarcinoma, WIF-1 facilitates tumorigenesis. WIF-1 is also expressed in mammalian retina and may play a role in photoreceptor development. The protein is synthesized as a 379 amino acid (aa) molecule that contains a signal sequence, a 150 aa WIF domain, 5 EGF-like repeats, and a hydrophilic domain at the carboxy terminus.
General readings
Park JH., et al. (2014) Dev. Biol. 386(1):227-236.Stock M., et al. (2013) Arthritis Rheum. 65(9):2310-2322.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 polyclonal antibody ( Cat # PAB2563 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of WIF1 ( arrow ) using rabbit WIF1 polyclonal antibody ( Cat # PAB2563 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the WIF1 gene ( Lane 2 ) ( Origene Technologies ) .
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 polyclonal antibody ( Cat # PAB2564 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of WIF1 ( arrow ) using WIF1 polyclonal antibody ( Cat # PAB2564 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the WIF1 gene ( Lane 2 ) ( Origene Technologies ) .
Western Blot analysis of WIF1 expression in transfected 293T cell line (H00011197-T01) by WIF1 MaxPab polyclonal antibody.Lane 1: WIF1 transfected lysate(41.69 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of WIF1 expression in transfected 293T cell line (H00011197-T01) by WIF1 MaxPab polyclonal antibody.Lane 1: WIF1 transfected lysate(41.50 KDa).Lane 2: Non-transfected lysate.
Human Muscle; WB Suggested Anti-WIF1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; WIF1 antibody - N-terminal region (ARP52298_P050) in Human Muscle cells using Western Blot
Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 antibody Cat.-No AM06523SU-N with DAB staining.
Immunofluorescence analysis of Hela cells using WIF1 antibody Cat.-No AM06523SU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using WIF1 antibody Cat.-No AM06523SU-N against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.
Western blot analysis of WIF1 (arrow) using rabbit polyclonal WIF1 Antibody (N-term) (Cat.#TA302106).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 (Human C-term) (Cat.#TA302106), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of WIF1 (arrow) using rabbit polyclonal WIF1 (C-term) Antibody (Cat.#TA302107). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 Antibody (C-term) (Cat.#TA302107), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of extracts of various cell lines, using WIF1 antibody.
Immunofluorescence analysis of U20S cell using WIF1 antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-WIF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Muscle
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302106 | WIF1 (N-term) antibody |
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| Rabbit Polyclonal Antibody against WIF1 (N-term) | |||||||
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA302107 | WIF1 (C-term) antibody |
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| Rabbit Polyclonal Antibody against WIF1 (C-term) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA332658 | WIF1 antibody |
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| Rabbit anti-WIF1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
||
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM06523SU-N | WIF1 antibody |
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 |
Mouse | IgG1 | 1G5 | Ascites | Hu | E, ICC/IF, P, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AM33421PU-N | WIF1 antibody |
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Mouse | IgG1 | 6D27 | Purified | Hu | P, WB |
0.1 mg /
€460.00
|
| OriGene Technologies GmbH | |||||||
| DM3608P | WIF1 antibody |
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Rat | IgG2 | 3M51 | Purified | Ms | WB |
0.1 mg /
€460.00
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305256 | WIF1 |
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| Recombinant protein of human WNT inhibitory factor 1 (WIF1) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP720656 | WIF1 |
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| Purified recombinant protein of Human WNT inhibitory factor 1 (WIF1) | |||||
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Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€299.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402102 | WIF1 overexpression lysate |
|
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR202510L2V | Lenti ORF particles, Wif1 (GFP-tagged) - Mouse Wnt inhibitory factor 1 (cDNA clone MGC:7686 IMAGE:3497155), 200 uL, >10^7 TU/mL |
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200 µl /
€840.00
|
|
| OriGene Technologies, Inc. | ||
| MR202510L1V | Lenti ORF particles, Wif1 (Myc-DDK-tagged) - Mouse Wnt inhibitory factor 1 (cDNA clone MGC:7686 IMAGE:3497155), 200 uL, >10^7 TU/mL |
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|
200 µl /
€740.00
|
|
| OriGene Technologies, Inc. | ||