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WNT1 antibody

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Principal name

WNT1 antibody

Alternative names for WNT1 antibody

Proto-oncogene protein Wnt-1, INT1, INT-1

SwissProt ID

P04426 (Mouse), P04628 (Human), P10108 (Xenla), P24257 (Danre), Q91029 (Chick)

Gene ID

7471 (WNT1), 22408 (Wnt1)

Ncbi ID

NP_005421.1, 9606, NP_005421, NM_005430, NP_067254.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Macaque, Op (Opossum), Can (Canine), Ze (Zebrafish), Mky (Monkey), Por (Porcine), Hst (Hamster)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of WNT1 antibody

WNT1 is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted sigling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT1 is very conserved in evolution, and it is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. WNT1 was origilly considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. WNT1 is clustered with another family member, WNT10B, in the chromosome 12q13 region.The WNT gene family consists of structurally related genes which encode secreted sigling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was origilly considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

PP1231P1 - WNT1
Formalin-Fixed, Paraffin-Embedded Sections of human breast invasive ductal carcinoma stained with WNT1 Antibody Cat.-No PP1231P
PP1231P1 - WNT1
Sandwich ELISA using WNT1 Antibody Cat.-No PP1231P
AP06617PU-N - WNT1
Immunohistochemistry (IHC) analyzes of Wnt-1 antibody (Cat.-No.: AP06617PU-N) in paraffin-embedded Human breast carcinoma tissue.
AP06617PU-N - WNT1
Western blot (WB) analysis of Wnt-1 antibody (Cat.-No.: AP06617PU-N) at 1/500 dilution
Lane1: HEK293T whole cell lysate
Lane2: H9C2 whole cell lysate
Lane3: sp2/0 whole cell lysate
AP15858PU-S - WNT1
AP15858PU Wnt1 antibody staining of Human Breast Carcinoma.
AP15858PU-S - WNT1
AP15858PU Wnt1 antibody staining of NIH3T3 cell lysate by Western Blot.
PAB11291 - WNT1
Western blot using WNT1 polyclonal antibody ( Cat # PAB11291 ) shows detection of endogenous WNT1 in human-derived MCF-7 cell lysate ( lane 1 ) and mouse-derived 3T3 cell lysate ( lane 2 ).The band at ~41 kDa, indicated by the arrowhead, corresponds to WNT1.After transfer, the membrane was blocked with 5% BLOTTO.Primary antibody was used at a 1 : 1,400 dilution in PBS containing 1% BLOTTO.The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with WNT1 ( data not shown ).
PAB9582 - WNT1
Westrn blot analysis of WNT1 expression in Jurkat lysate ( Lane 1 and lane 2 ).
AP17833PU-N - WNT1
Western blot analysis of WNT1 Antibody (C-term) (AP17833PU-N) in mouse heart tissue lysates (35ug/lane). WNT1 (arrow) was detected using the purified Pab.
ARP32122_P050 - WNT1
Human H9; . Sample Type: 1. Molecular Weight. 2. Control (20ug). 3. shRNA1-WNT1 H9 hES cells (20ug). 4. shRNA2-WNT1 H9 hES cells (20ug). Primary Dilution: 1:1000. Secondary Antibody: anti-Rabbit HRP. Secondary Dilution: 1:5000. Image Submitted By: Jingli Cai. Thomas Jefferson University .; WNT1 antibody - middle region (ARP32122_P050) in Human H9 cells using Western Blot
ARP32122_P050 - WNT1
Human Muscle; WB Suggested Anti-WNT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle; WNT1 antibody - middle region (ARP32122_P050) in Human Muscle cells using Western Blot
C0392-1 - WNT1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using WNT1 Antibody. The picture on the right is treated with the synthesized peptide.
AM06361SU-N - WNT1
Immunohistochemical analysis of paraffin-embedded human LAdrenal tissues using WNT1 mouse mAb.
AM06361SU-N - WNT1
Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
AM06361SU-N - WNT1
Western blot analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.
AM06361SU-N - WNT1
Flow cytometric analysis of Hela cells using WNT1 mouse mAb (green) and negative control (purple).
CPA2232-100ul - WNT1
Immunohistochemical analysis of WNT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA2232-100ul - WNT1
Immunofluorescent analysis of WNT1 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA2232-100ul - WNT1
Western blot analysis of WNT1 expression in HEK293T (A); SP2/0 (B); H9C2 (C); WI38 (D); NIH3T3 (E) whole cell lysates.
TP723481 - WNT1
TA314674 - WNT1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using WNT1 antibody.The picture on the right is treated with the synthesized peptide.
TA319125 - WNT1
Western blot analysis using recombinant human Wnt-1. Lane 1: 5 ng, Lane2: 10 ng, Lane 3: 50 ng.
TA319438 - WNT1
WB using Anti-Wnt1 antibody shows detection of endogenous Wnt1 in human-derived MCF7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kDa, indicated by the arrowhead, corresponds to Wnt1. After transfer, the membrane was blocked with 5% BLOTTO. Primary antibody was used at a 1:1,400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt1 (data not shown).
TA319584 - WNT1
Western blot using Protein A purified anti-Wnt1 monoclonal antibody shows detection of Wnt1 protein in mouse testis lysate. The results show specific binding corresponding to the ~41 kDa Wnt1 protein. Primary antibody was used at a 1:500 dilution. Personal communication, Stephen Brown, Brown University
TA328474 - WNT1
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 62.5 ng/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
TA329904 - WNT1
WB Suggested Anti-WNT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Muscle
TA329904 - WNT1
Sample Type: 1. Molecular Weight; 2. Control (20 ug); 3. shRNA1-WNT1 H9 hES cells (20 ug); 4.shRNA2-WNT1 H9 hES cells (20ug); Primary Dilution: 1:1000; Secondary Antibody: anti-Rabbit HRP; Secondary Dilution: 1:5000; Image Submitted By: Jingli Cai; Thomas
TA329905 - WNT1
Host: Rabbit; Target Name: Wnt1; Sample Tissue: Mouse Liver lysates; Antibody Dilution: 1.0ug/ml
TA341203 - WNT1
Anti-WNT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA341203 - WNT1
Anti-WNT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TA341204 - WNT1
Anti-WNT1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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  2. Cited (1)
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16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WNT1 antibody
Immunohistochemistry (IHC) analyzes of Wnt-1 antibody (Cat.-No.: AP06617PU-N) in paraffin-embedded Human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH
+1 additional image

Published recently!

WNT1 antibody
Immunohistochemical analysis of paraffin-embedded human LAdrenal tissues using WNT1 mouse mAb. Mouse IgG1 10C8 Ascites Hu, Ms E, ICC/IF, P, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

WNT1 antibody
  Rabbit polyclonal anti-WNT1 antibody  
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using WNT1 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

WNT1 antibody
  Rabbit polyclonal anti-Wnt-1 antibody  
Western blot analysis using recombinant human Wnt-1. Lane 1: 5 ng, Lane2: 10 ng, Lane 3: 50 ng. Rabbit Purified Hu WB
0.1 mg / €355.00
  OriGene Technologies, Inc.

WNT1 antibody
  Rabbit polyclonal anti-Wnt1 antibody  
WB using Anti-Wnt1 antibody shows detection of endogenous Wnt1 in human-derived MCF7 cell lysate (lane 1) and mouse-derived 3T3 cell lysate (lane 2). The band at ~41 kDa, indicated by the arrowhead, corresponds to Wnt1. After transfer, the membrane was blocked with 5% BLOTTO. Primary antibody was used at a 1:1,400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt1 (data not shown). Rabbit IgG Aff - Purified Bov, Can, Hu, Mky, Ms, Op, Rt E, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

WNT1 antibody
  Mouse monoclonal anti-Wnt1 antibody  
Western blot using Protein A purified anti-Wnt1 monoclonal antibody shows detection of Wnt1 protein in mouse testis lysate. The results show specific binding corresponding to the ~41 kDa Wnt1 protein. Primary antibody was used at a 1:500 dilution. Personal communication, Stephen Brown, Brown University Mouse 13F9 Purified Hu E, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

WNT1 antibody
  Anti-Human WNT-1 Rabbit Polyclonal Antibody  
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 62.5 ng/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €275.00
  OriGene Technologies, Inc.

WNT1 antibody
  Rabbit Polyclonal Anti-WNT1 Antibody (Internal)  
Anti-WNT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Chk, Hst, Hu, Mky
50 µg / €425.00
  OriGene Technologies, Inc.
+1 additional image

WNT1 antibody
  Rabbit Polyclonal Anti-WNT1 Antibody (Internal)  
Anti-WNT1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €425.00
  OriGene Technologies, Inc.

WNT1 (C-term) antibody
Western blot analysis of WNT1 Antibody (C-term) (AP17833PU-N) in mouse heart tissue lysates (35ug/lane). WNT1 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €415.00
  OriGene Technologies GmbH

WNT1 antibody
WNT1 Rabbit Biotin Hu E, WB
25 µg / €250.00
  OriGene Technologies GmbH

WNT1 antibody
WNT1 Rabbit Biotin Hu E, WB
50 µg / €340.00
  OriGene Technologies GmbH

WNT1 antibody
Formalin-Fixed, Paraffin-Embedded Sections of human breast invasive ductal carcinoma stained with WNT1 Antibody Cat.-No PP1231P Rabbit Aff - Purified Hu E, P, WB
50 µg / €250.00
  OriGene Technologies GmbH
+1 additional image

WNT1 antibody
Formalin-Fixed, Paraffin-Embedded Sections of human breast invasive ductal carcinoma stained with WNT1 Antibody Cat.-No PP1231P Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €340.00
  OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WNT1
  Purified recombinant protein of Human wingless-type MMTV integration site family, member 1 (WNT1).  
WNT1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.		 E. coli
10 µg / €230.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

WNT1 overexpression lysate
WNT1 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

16 Item(s)

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  1. Lysate (1)
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Target
  1. WNT1 (15)
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Reactivity
  1. Human (Hu) (14)
  2. Mouse (Ms) (5)
  3. Monkey (Mky) (2)
  4. Bovine (Bov) (1)
  5. Canine (Can) (1)
  6. Chicken (Chk) (1)
  7. Hamster (Hst) (1)
  8. Opossum (Op) (1)
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Applications
  1. Western blot / Immunoblot (WB) (12)
  2. Enzyme Immunoassay (E) (9)
  3. Paraffin Sections (P) (6)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
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