WNT3 antibody
Principal name
WNT3 antibody
Alternative names for WNT3 antibody
Proto-oncogene protein Wnt-3, INT4, INT-4
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of WNT3 antibody
Wnts have diverse roles in governing cell fate, proliferation, migration, and polarity during development, particularly in embryogenesis and carcinogenesis. WNTs are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity pathways. Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of these pathways and lead to unique cellular responses. Wnt3 is expressed in embryonic mouse brain (with peak expression at around day 12 of gestation) and at low levels in the adult.
It is present in the cerebellum during mouse development, and is restricted to the Purkinje cell layer in the adult mouse. Wnt3 is required for normal limb development and plays an important role in the establishment of the apical ectodermal ridge. In motorneurons, Wnt3 is a retrograde signal that controls terminal branching of muscle afferents. Wnt3 is highly expressed in human cancer cell lines including A549 (lung cancer), MKN45 (gastric cancer) and MCF7 (breast cancer).
Wnt3 and Wnt3a are nearly 90% identical at the amino acid level.
WNT3 polyclonal antibody ( Cat # PAB7032 ) ( 1 µg/ml ) staining of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of WNT3 expression in transfected 293T cell line (H00007473-T01) by WNT3 MaxPab polyclonal antibody.Lane 1: WNT3 transfected lysate(39.60 KDa).Lane 2: Non-transfected lysate.
Human Thyroid (formalin-fixed, paraffin-embedded) stained with WNT3 antibody AP22539PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Breast (formalin-fixed, paraffin-embedded) stained with WNT3 antibody AP22539PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Adrenal (formalin-fixed, paraffin-embedded) stained with WNT3 antibody AP22539PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Antibody (1 µg/ml) staining of Human Brain lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Human Brain; WB Suggested Anti-WNT3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; WNT3 antibody - middle region (ARP49956_P050) in Human Brain cells using Western Blot
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801734)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801734)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801734)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801734)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801736)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801736)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801867)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801867)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801867)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801867)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801867)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801867)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801867)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801892)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801892)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-WNT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801892)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3 (RC211115, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WNT3.
TA302636 (1µg/ml) staining of lysate of cell line HEK293 (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA302636 (2µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN211115LP | WNT3 - human gene knockout kit via CRISPR |
||
 |
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
