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WNT5A antibody

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Principal name

WNT5A antibody

Alternative names for WNT5A antibody

Protein Wnt-5a

SwissProt ID

P22725 (Mouse), P31286 (Xenla), P41221 (Human), Q27Q52 (Rabit), Q9QXQ7 (Rat)

Gene ID

7474 (WNT5A), 22418 (Wnt5a), 64566

Ncbi ID

9606, NP_003383, NP_003383.2, NP_033550.2, NM_009524, NM_022631, NM_003392

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rb (Rabbit), Rt (Rat), African clawed frog, Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of WNT5A antibody

The WNT gene family consists of structurally related genes which encode secreted sigling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT5A is a member of the WNT protein family. It shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 sigling mediates axis induction.The WNT gene family consists of structurally related genes which encode secreted sigling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 sigling mediates axis induction. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

H00007474-D01P - WNT5A
Proximity Ligation Analysis (PLA) of protein-protein interactions between WNT5A and FZD5 HeLa cells were stained with anti-WNT5A rabbit purified polyclonal 1:1200 and anti-FZD5 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
H00007474-D01P - WNT5A
Western Blot analysis of WNT5A expression in transfected 293T cell line (H00007474-T02) by WNT5A MaxPab polyclonal antibody.Lane 1: WNT5A transfected lysate(42.30 KDa).Lane 2: Non-transfected lysate.
ARP32128_P050 - WNT5A
Human Brain; WNT5A antibody - middle region (ARP32128_P050) in Human Brain cells using Immunohistochemistry
ARP32128_P050 - WNT5A
Human Colon; WNT5A antibody - middle region (ARP32128_P050) in Human Colon cells using Immunohistochemistry
ARP32128_P050 - WNT5A
Human Placenta; WNT5A antibody - middle region (ARP32128_P050) in Human Placenta cells using Immunohistochemistry
ARP32128_P050 - WNT5A
Human HeLa; WB Suggested Anti-WNT5A Antibody Titration: 1 ug/ml. Positive Control: Hela cell lysate. WNT5A is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; WNT5A antibody - middle region (ARP32128_P050) in Human HeLa cells using Western Blot
AM06324SU-N - WNT5A
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A antibody Cat.-No AM06324SU-N with DAB staining.
AM06324SU-N - WNT5A
Confocal Immunofluorescence analysis of PC-12 cells using WNT5A antibody Cat.-No AM06324SU-N (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
AM06324SU-N - WNT5A
Western blot analysis using WNT5A antibody Cat.-No AM06324SU-N against HEK293 (Lane 1) and WNT5A-hIgGFc transfected HEK293 cell lysate (Lane 2).
AM06331SU-N - WNT5A
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A antibody Cat.-No AM06331SU-N with DAB staining.
AM06331SU-N - WNT5A
Western blot analysis using WNT5A antibody Cat.-No AM06331SU-N against HEK293 (Lane 1) and WNT5A-hIgGFc transfected HEK293 cell lysate (Lane 2).
TP309206 - WNT5A
TA324222 - WNT5A
Predicted band size: 42 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA329908 - WNT5A
WB Suggested Anti-WNT5A Antibody Titration: 1 ug/ml; Positive Control: Hela cell lysateWNT5A is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells
TA329908 - WNT5A
Human Placenta
TA329908 - WNT5A
Human Colon
TA329908 - WNT5A
Human Brain
TA337059 - WNT5A
Western Blot: Wnt5a Antibody [TA337059] - analysis of Wnt5A in human heart lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat heart using Wnt5A antibody at 0.5 ug/ml.
TA337059 - WNT5A
Immunohistochemistry-Paraffin: Wnt5a Antibody [TA337059] - Analysis of Wnt5A in formalin-fixed, paraffin-embedded human breast tumor using an isotype control (left) and Wnt5A antibody (right) at 10 ug/ml.
TA337152 - WNT5A
Immunohistochemistry-Paraffin: Wnt5a Antibody (4M5E4) [TA337152] - Staining of human breast tumor tissue probed with Wnt5A antibody at 5 ug/ml.

18 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

WNT5A antibody
  Anti-WNT5A Rabbit Polyclonal Antibody  
Predicted band size: 42 kDa . Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

WNT5A antibody
  Rabbit Polyclonal Wnt-5a Antibody  
Western Blot: Wnt5a Antibody [TA337059] - analysis of Wnt5A in human heart lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat heart using Wnt5A antibody at 0.5 ug/ml. Rabbit IgG Purified Bov, Hu, Ms, Prim, Rt P, WB
0.1 mg / €450.00
  OriGene Technologies, Inc.
+1 additional image

WNT5A antibody
  Mouse Monoclonal Wnt-5a Antibody (4M5E4)  
Immunohistochemistry-Paraffin: Wnt5a Antibody (4M5E4) [TA337152] - Staining of human breast tumor tissue probed with Wnt5A antibody at 5 ug/ml. Mouse IgG2a 4M5E4 Purified Hu P
0.1 mg / €395.00
  OriGene Technologies, Inc.

WNT5A antibody
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A antibody Cat.-No AM06324SU-N with DAB staining. Mouse IgG1 6F2 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
+2 additional images

WNT5A antibody
Immunohistochemical analysis of paraffin-embedded human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) showing cytoplasmic and extracellular matrix localization using WNT5A antibody Cat.-No AM06331SU-N with DAB staining. Mouse IgG1 3D10 Ascites Hu E, P, WB
0.1 ml / €380.00
  OriGene Technologies GmbH
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

WNT5A - human gene knockout kit via CRISPR
WNT5A - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

WNT5A - human gene knockout kit via CRISPR
WNT5A - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

WNT5A - human gene knockout kit via CRISPR
WNT5A - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

WNT5A - human gene knockout kit via CRISPR
WNT5A - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Wnt5a - mouse gene knockout kit via CRISPR
Wnt5a - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Wnt5a - mouse gene knockout kit via CRISPR
Wnt5a - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Wnt5a - mouse gene knockout kit via CRISPR
Wnt5a - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Wnt5a - mouse gene knockout kit via CRISPR
Wnt5a - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

WNT5A
  Recombinant protein of human wingless-type MMTV integration site family, member 5A (WNT5A)  
WNT5A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Wnt5a (GFP-tagged) - Mouse wingless-related MMTV integration site 5A (Wnt5a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Wnt5a (GFP-tagged) - Mouse wingless-related MMTV integration site 5A (Wnt5a), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Wnt5a (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 5A (Wnt5a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Wnt5a (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 5A (Wnt5a), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Wnt5a (GFP-tagged ORF) - Rat wingless-type MMTV integration site family, member 5A (Wnt5a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Wnt5a (GFP-tagged ORF) - Rat wingless-type MMTV integration site family, member 5A (Wnt5a), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Wnt5a (Myc-DDK-tagged ORF) - Rat wingless-type MMTV integration site family, member 5A (Wnt5a), 200 uL, >10^7 TU/mL
Lenti ORF particles, Wnt5a (Myc-DDK-tagged ORF) - Rat wingless-type MMTV integration site family, member 5A (Wnt5a), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

18 Item(s)

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18 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (8)
  2. Mouse Lenti ORF Particle (2)
  3. Primary Antibody (5)
  4. Protein | Growth Factor (1)
  5. Rat Lenti ORF Particle (2)
Clonality
  1. Monoclonal (3)
  2. Polyclonal (2)
Target
  1. WNT5A (6)
Reactivity
  1. Human (Hu) (5)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
  4. Bovine (Bov) (1)
  5. Primate (Prim) (1)
Applications
  1. Paraffin Sections (P) (4)
  2. Western blot / Immunoblot (WB) (4)
  3. Enzyme Immunoassay (E) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (3)
  2. Ascites (2)
Host
  1. Mouse (3)
  2. Rabbit (2)
Images
  1. 1 image (6)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. IHC (2)
  3. Paraffin Sections (P) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Species (CRISPR)
  1. Human (4)
  2. Mouse (4)
Donor Vector
  1. GFP-Puro (2)
  2. Luciferase-Puro (2)
  3. RFP-BSD (2)
  4. mBFP-Neo (2)
Manufacturer
  1. OriGene Technologies (18)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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