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XPNPEP1 antibody

Principal name

XPNPEP1 antibody

Alternative names for XPNPEP1 antibody

XPNPEPL, XPNPEPL1, Xaa-Pro aminopeptidase 1, X-Pro aminopeptidase 1, X-prolyl aminopeptidase 1 soluble, Cytosolic aminopeptidase P, Soluble aminopeptidase P, Aminoacylproline aminopeptidase

SwissProt ID

O54975 (Rat), Q1JPJ2 (Bovin), Q6P1B1 (Mouse), Q9NQW7 (Human)

Gene ID

7511 (XPNPEP1), 170751 (Xpnpep1), 170750

Ncbi ID

9606, NP_571988.1, NP_001161076.1, NP_065116, NM_020383, NM_001167604, NP_001161076, NP_573479.2, NM_131913

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine)

Available hosts

Mouse, Goat

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Paraffin Sections (P)

Background of XPNPEP1 antibody

Aminopeptidase P (AP-P; X-Pro aminopeptidase) has the unique ability to leave the N-terminal amino acid residue from peptides having proline as the penultimate amino acid residue. Biologically active peptides comprise an important and diverse class of extracellular chemical messengers that mediate a wide range of intercellular interactions. Several bioactive peptides including hormones, neuropeptides, neurotransmitters escape non-specific protease degradation by having an Xaa-Pro motif at their amino termini. Due to its cyclic nature, proline confers resistance to such peptide bonds so that aminopeptidases with broad specificity cannot act upon such peptides. There are a limited number of peptidases that act on peptide bonds involving a proline residue, such as dipeptidyl peptidase II (DPPII) and dipeptidyl peptidase W (DPPIV), and prolidase (which cleaves the Xaa-Pro bond only in dipeptides), or endopeptidases such as prolyl endopeptidase (which cleaves on the carbonyl side of proline residues within a protein or peptide). However, none of these enzymes have been reported to hydrolyze Xaa-Pro bonds located at the N-terminus of peptides and proteins. Therefore, role of AP-P is crucial in this respect. AP-P activity is ubiquitous and has been found in a wide range of organisms including bacteria, yeast and vertebrates. Mammalian AP-Ps exist in membrane-bound and cytosolic forms, which represent two distinct gene products. The cytosolic (soluble) form of aminopeptidase P is found in human leukocytes and rat brain.

General readings

Cottrell G.S., et al. (2000) Biochemistry 39:15121-15128.Li X., et al. (2008) J. Biol. Chem. 283:22858-22866.

XPNPEP1 MaxPab polyclonal antibody. Western Blot analysis of XPNPEP1 expression in human pancreas.

H00007511-B01

Western Blot analysis of XPNPEP1 expression in transfected 293T cell line (H00007511-T01) by XPNPEP1 MaxPab polyclonal antibody.Lane 1: XPNPEP1 transfected lysate(68.53 KDa).Lane 2: Non-transfected lysate.

H00007511-B01

Staining of Aminopeptidase P in the tubulointerstitial blood vessels and in particular the blood vessels of the glomerulus (paraffin-embedded tissue-sections fixed in formalin) with anti-Rat Aminopeptidase-P Cat.-No DM3611P

DM3611P

Immunofluorescence staining of endothelial cells of the glomerulus as well as the tubulointerstitial blood vessels (here very weak) (paraffinembedded tissue-sections fixed in formalin) with anti-Rat Aminopeptidase-P Cat.-No DM3611P

DM3611P

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-XPNPEP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501383, Dilution 1:50)

TA501383

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-XPNPEP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501383, Dilution 1:50)

TA501383

Anti-XPNPEP1 mouse monoclonal antibody (TA501383) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP1(RC203535).

TA501383

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XPNPEP1 (RC203535, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPNPEP1.

TA501383

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-XPNPEP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

TA501383

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-XPNPEP1 mouse monoclonal antibody. (TA501384)

TA501384

Anti-XPNPEP1 mouse monoclonal antibody (TA501384) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP1(RC203535).

TA501384

TA501384

TP303535

TP720170

TA305684 (0.1 ug/ml) staining of Mouse (A) and Rat (B) Small Intestine lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TA305684

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA501383	XPNPEP1 antibody
	anti-XPNPEP1 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)
	Mouse	IgG1	OTI1E3	Purified	Hu	ICC/IF, P, WB	0.1 ml / €379.00
							OriGene Technologies, Inc.
+4 additional images	Highly validated antibody!
TA305684	XPNPEP1 (C-term) antibody
	Goat Anti-XPNPEP1 Antibody
	Goat			Aff - Purified	Bov, Can, Hu, Ms, Por, Rt	WB	0.1 mg / €360.00
							OriGene Technologies, Inc.
TA501384	XPNPEP1 antibody
	anti-XPNPEP1 mouse monoclonal antibody, clone OTI1E7 (formerly 1E7)
	Mouse	IgG1	OTI1E7	Purified	Hu	ICC/IF, P, WB	0.1 ml / €360.00
							OriGene Technologies, Inc.
+2 additional images
DM3611P	XPNPEP1 antibody
	Mouse	IgG1	JG12C9C10	Purified	Rt	C, ICC/IF, P	0.1 mg / €350.00
							OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP303535	XPNPEP1
	Recombinant protein of human X-prolyl aminopeptidase (aminopeptidase P) 1, soluble (XPNPEP1)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

TP720170	XPNPEP1 (transcript variant 2)
	Recombinant protein of human X-prolyl aminopeptidase (aminopeptidase P) 1, soluble (XPNPEP1), transcript variant 2.
	Human	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	10 µg / €299.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY402779	XPNPEP1 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
	Published recently!
LY433385	XPNPEP1 overexpression lysate
		0.1 mg / €315.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR201588L2V	Lenti ORF particles, Xpnpep1 (GFP-tagged ORF) - Rat X-prolyl aminopeptidase (aminopeptidase P) 1, soluble (Xpnpep1), 200 uL, >10^7 TU/mL
		200 µl / €1,360.00
		OriGene Technologies, Inc.

RR201588L1V	Lenti ORF particles, Xpnpep1 (Myc-DDK-tagged ORF) - Rat X-prolyl aminopeptidase (aminopeptidase P) 1, soluble (Xpnpep1), 200 uL, >10^7 TU/mL
		200 µl / €1,220.00
		OriGene Technologies, Inc.

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